Effect of vitrification on spermatozoa quality in bull semen
Cryopreservation of spermatozoa is part of the assisted reproductive biotechnology to enhance reproductive capacity in livestock. Conventional cryopreservation applies slow-gradual freezing method permitted ice crystallization and causes cryodamage resulting in poor post-thawed semen quality....
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| Format: | Project Paper Report |
| Language: | English |
| Published: |
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/78297/ http://psasir.upm.edu.my/id/eprint/78297/1/FPV%202017%2044%20-%20IR.pdf |
| Summary: | Cryopreservation of spermatozoa is part of the assisted reproductive
biotechnology to enhance reproductive capacity in livestock. Conventional
cryopreservation applies slow-gradual freezing method permitted ice crystallization
and causes cryodamage resulting in poor post-thawed semen quality. Hence,
vitrification is introduced by solidifying the solution into glassy state without
causing any crystallization in fast and inexpensive manner. This ultra-rapid cooling
method requires high concentration of cryoprotectants that potentially toxic the
spermatozoa. Therefore, this study was conducted to determine the effect of
vitrification on the quality of bull semen. A total of eight (8) bull semen samples
were collected using electro-ejaculation. Tris-based extender was compared with
vitrification using solution of different concentration of cryoprotectants at 10% (Vitrification Solution 1; VS-1) and 20% (VS-2) containing dimethyl sulfoxide
(DMSO) and ethylene glycol respectively. The result revealed that high mortality
and nearly zero motility in all post-warmed vitrified spermatozoa, but the general
and progressive motilities parameters in VS-1 at initial evaluation was 22.45% and
24.87% respectively better than Tris-based extender and was statistically significant.
In conclusion, vitrification has potential as an alternative for cryopreservation.
Therefore, further research on spermatozoa vitrification technique on enhancement
in cooling and warming should be conducted and investigated. |
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