Development of polymerase chain reaction (PCR) technique for detection of Mycoplasma hyopneumoniae and Pseudorabies virus in porcine clinical samples
With recent advancement in molecular biology, molecular detection has become an alternative diagnostic technique. As Aujeszky’s disease and Enzootic Pneumonia caused great economic losses in swine livestock industry, this study aimed to determine suitable primers sets for Pseudorabies virus (P...
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| Format: | Project Paper Report |
| Language: | English |
| Published: |
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/78257/ http://psasir.upm.edu.my/id/eprint/78257/1/FPV%202016%2099%20-%20IR.pdf |
| Summary: | With recent advancement in molecular biology, molecular detection has
become an alternative diagnostic technique. As Aujeszky’s disease and Enzootic
Pneumonia caused great economic losses in swine livestock industry, this study
aimed to determine suitable primers sets for Pseudorabies virus (PRV) and
Mycoplasma hyopneumoniae (Mhyo) detection in clinical samples using PCR. 15
pigs aged approximately 3 months old showing clinical signs of respiratory distress
were sampled. Commercial vaccine and positive clinical samples were used as
positive control. Each lung and tonsil tissue samples were subjected to conventional PCR assay using 3 different sets of primers designed to target conserved regions of
genomic DNA for Mhyo and PRV respectively. Based on PCR assay for lung tissue
samples, 3 out of 15 pigs were positive for Mhyo with all 3 primers sets whereas for
tonsil tissue samples, all 15 pigs were negative for PRV. To further optimize the
current protocol, more sample size and gradient PCR assay should be performed. In
conclusion, all the primers sets chosen were suitable to be used for Mhyo and PRV
detection using PCR. |
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