Plasma bovine growth hormone (bGH) in buffaloes and its associations with selected phenotypes
Forty buffaloes with different phenotypes were used in the study. The phenotypes were the breed, sex, current body weight, age, birth weight and age at puberty. Blood samples were collected from the coccygeal vein into ethylenediamine tetraacetic acid (EDTA) tubes. Plasma was harvested after...
| Main Author: | |
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| Format: | Project Paper Report |
| Language: | English |
| Published: |
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/78223/ http://psasir.upm.edu.my/id/eprint/78223/1/FPV%202016%2086%20-%20IR.pdf |
| Summary: | Forty buffaloes with different phenotypes were used in the study. The
phenotypes were the breed, sex, current body weight, age, birth weight and age
at puberty. Blood samples were collected from the coccygeal vein into
ethylenediamine tetraacetic acid (EDTA) tubes. Plasma was harvested after
centrifugation at 3000 rpm for 15 minutes. Concentrations of plasma bovine
growth hormone (bGH) were determined by quantitative sandwich ELISA
using commercial kits from Novateinbio Bovine Growth Hormone ELISA Kit. The data on selected phenotypes were collected from the farm records
retrospectively between 2006 and 2015. Breeds wise, there was no significant
difference (p>0.05) among Swamp and Murrah Cross in plasma bGH
concentration. There was also no significant difference (p>0.05) among female
and male buffaloes in plasma bGH concentration. In age groups, there was no
significant difference (p>0.05) among young, adult and old buffaloes in plasma
bGH concentration. The correlation coefficient between (r) between plasma
bGH concentration, body weight, age, birth weight and age at puberty were
tested which plasma bGH concentration was negatively correlated with body
weight (r = -0.069), age (r = -0.111), birth weight(r = -0.141) and age at puberty
(r = -0.062). Thus, in this study, plasma bGH concentration did not showed any
association with selected phenotypes. |
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