Detection of Salmonella and E.coli in Edible Bird's Nest ranched in housing system
The swiftlet industry in Malaysia is growing very fast due to the high demand for Edible Bird’s Nest (EBN) . The presence of bacteria may produce low quality of EBN that can lead to economic loss and may cause food-borne diseases. The aim of this study was to detect the presence of Salmonell...
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| Format: | Project Paper Report |
| Language: | English |
| Published: |
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/78215/ http://psasir.upm.edu.my/id/eprint/78215/1/FPV%202016%2063%20IR.pdf |
| Summary: | The swiftlet industry in Malaysia is growing very fast due to the high demand
for Edible Bird’s Nest (EBN) . The presence of bacteria may produce low quality of
EBN that can lead to economic loss and may cause food-borne diseases. The aim of
this study was to detect the presence of Salmonella and E.coli in EBN and guano of
edible-nest swiftlets ranched in housing system and to enumerate the total number of
bacteria and coliform in EBN. In this study, a total of 64 guano and nest swab
samples were collected from three bird houses in Terengganu. The samples were
pre-enriched and enriched before culturing on Brilliant Green Agar (BGA) and Xylose Lysine Deoxycholate (XLD) for isolation of Salmonella and propagated in
nutrient broth before culture on Eosin Methylene Blue Agar (EMBA) for isolation of
E.coli. The isolation of the bacteria was carried out in Veterinary Public Health
Laboratory, Faculty of Veterinary Medicine, UPM. Standard Plate Count (SPC) and
Coliform count using 3M® Petrifilm were done. No Salmonella was isolated in EBN
and the prevalence of Salmonella in guano was 12.5% as compared to E. coli in EBN
at 3.13% and 68.75% in guano sample. The average SPC of EBNs was 3.2 x 105
CFU per gram and CPC was ≤ 100 CFU per gram . E.coli in EBNs swab were lower
than in guano samples. Hence, the absence of Salmonella and low number of E.coli
in EBN is a good news for the growing swiftlet industry. |
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