Genotoxicity of Euphorbia hirta: an Allium cepa assay
The potential genotoxic effects of methanolic extracts of Euphorbia hirta which is commonly used in traditional medicine to treat a variety of diseased conditions including asthma, coughs, diarrhea and dysentery was investigated using Allium cepa assay. The extracts of 125, 250, 500 and 1,000 µg/mL...
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| Format: | Article |
| Language: | English |
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MDPI
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/77988/ http://psasir.upm.edu.my/id/eprint/77988/1/77988.pdf |
| _version_ | 1848858381993902080 |
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| author | Kwan, Yuet Ping Ibrahim, Darah Yusuf, Umi Kalsom Chen, Yeng Sreenivasan, Sasidharan |
| author_facet | Kwan, Yuet Ping Ibrahim, Darah Yusuf, Umi Kalsom Chen, Yeng Sreenivasan, Sasidharan |
| author_sort | Kwan, Yuet Ping |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The potential genotoxic effects of methanolic extracts of Euphorbia hirta which is commonly used in traditional medicine to treat a variety of diseased conditions including asthma, coughs, diarrhea and dysentery was investigated using Allium cepa assay. The extracts of 125, 250, 500 and 1,000 µg/mL were tested on root meristems of A. cepa. Ethylmethanesulfonate was used as positive control and distilled water was used as negative control. The result showed that mitotic index decreased as the concentrations of E. hirta extract increased. A dose-dependent increase of chromosome aberrations was also observed. Abnormalities scored were stickiness, c-mitosis, bridges and vagrant chromosomes. Micronucleated cells were also observed at interphase. Result of this study confirmed that the methanol extracts of E. hirta exerted significant genotoxic and mitodepressive effects at 1,000 µg/mL. |
| first_indexed | 2025-11-15T12:12:33Z |
| format | Article |
| id | upm-77988 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:12:33Z |
| publishDate | 2012 |
| publisher | MDPI |
| recordtype | eprints |
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| spelling | upm-779882020-06-01T03:12:45Z http://psasir.upm.edu.my/id/eprint/77988/ Genotoxicity of Euphorbia hirta: an Allium cepa assay Kwan, Yuet Ping Ibrahim, Darah Yusuf, Umi Kalsom Chen, Yeng Sreenivasan, Sasidharan The potential genotoxic effects of methanolic extracts of Euphorbia hirta which is commonly used in traditional medicine to treat a variety of diseased conditions including asthma, coughs, diarrhea and dysentery was investigated using Allium cepa assay. The extracts of 125, 250, 500 and 1,000 µg/mL were tested on root meristems of A. cepa. Ethylmethanesulfonate was used as positive control and distilled water was used as negative control. The result showed that mitotic index decreased as the concentrations of E. hirta extract increased. A dose-dependent increase of chromosome aberrations was also observed. Abnormalities scored were stickiness, c-mitosis, bridges and vagrant chromosomes. Micronucleated cells were also observed at interphase. Result of this study confirmed that the methanol extracts of E. hirta exerted significant genotoxic and mitodepressive effects at 1,000 µg/mL. MDPI 2012 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/77988/1/77988.pdf Kwan, Yuet Ping and Ibrahim, Darah and Yusuf, Umi Kalsom and Chen, Yeng and Sreenivasan, Sasidharan (2012) Genotoxicity of Euphorbia hirta: an Allium cepa assay. Molecules, 17 (7). pp. 7782-7791. ISSN 1420-3049 https://www.mdpi.com/1420-3049/17/7/7782 10.3390/molecules17077782 |
| spellingShingle | Kwan, Yuet Ping Ibrahim, Darah Yusuf, Umi Kalsom Chen, Yeng Sreenivasan, Sasidharan Genotoxicity of Euphorbia hirta: an Allium cepa assay |
| title | Genotoxicity of Euphorbia hirta: an Allium cepa assay |
| title_full | Genotoxicity of Euphorbia hirta: an Allium cepa assay |
| title_fullStr | Genotoxicity of Euphorbia hirta: an Allium cepa assay |
| title_full_unstemmed | Genotoxicity of Euphorbia hirta: an Allium cepa assay |
| title_short | Genotoxicity of Euphorbia hirta: an Allium cepa assay |
| title_sort | genotoxicity of euphorbia hirta: an allium cepa assay |
| url | http://psasir.upm.edu.my/id/eprint/77988/ http://psasir.upm.edu.my/id/eprint/77988/ http://psasir.upm.edu.my/id/eprint/77988/ http://psasir.upm.edu.my/id/eprint/77988/1/77988.pdf |