In vitro anti-diabetic activities and chemical analysis of polypeptide-k and oil isolated from seeds of Momordica charantia (bitter gourd)
The amino acid and fatty acid composition of polypeptide k and oil isolated from the seeds of Momordica charantia was analysed. The analysis revealed polypeptide k contained 9 out of 11 essential amino acids, among a total of 18 types of amino acids. Glutamic acid, aspartic acid, arginine and glycin...
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| Format: | Article |
| Language: | English |
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MDPI
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/77969/ http://psasir.upm.edu.my/id/eprint/77969/1/77969.pdf |
| _version_ | 1848858376522432512 |
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| author | Ahmad, Zuraini Zamhuri, Khairul Faizi Yaacob, Azhar Chiong, Hoe Siong Selvarajah, Malarvili Ismail, Amin Abdullah, Muhammad Nazrul Hakim |
| author_facet | Ahmad, Zuraini Zamhuri, Khairul Faizi Yaacob, Azhar Chiong, Hoe Siong Selvarajah, Malarvili Ismail, Amin Abdullah, Muhammad Nazrul Hakim |
| author_sort | Ahmad, Zuraini |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The amino acid and fatty acid composition of polypeptide k and oil isolated from the seeds of Momordica charantia was analysed. The analysis revealed polypeptide k contained 9 out of 11 essential amino acids, among a total of 18 types of amino acids. Glutamic acid, aspartic acid, arginine and glycine were the most abundant (17.08%, 9.71%, 9.50% and 8.90% of total amino acids, respectively). Fatty acid analysis showed unusually high amounts of C18-0 (stearic acid, 62.31% of total fatty acid). C18-1 (oleic acid) and C18-2 (linoleic acid) were the other major fatty acid detected (12.53% and 10.40%, respectively). The oil was devoid of the short fatty acids (C4-0 to C8-0). Polypeptide k and oil were also subjected to in vitro α-glucosidase and α-amylase inhibition assays. Both polypeptide k and seed oil showed potent inhibition of α-glucosidase enzyme (79.18% and 53.55% inhibition, respectively). α-Amylase was inhibited by 35.58% and 38.02%, respectively. Collectively, the in vitro assay strongly suggests that both polypeptide k and seed oil from Momordica charantia are potent potential hypoglycemic agents. |
| first_indexed | 2025-11-15T12:12:28Z |
| format | Article |
| id | upm-77969 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:12:28Z |
| publishDate | 2012 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-779692020-05-04T17:54:56Z http://psasir.upm.edu.my/id/eprint/77969/ In vitro anti-diabetic activities and chemical analysis of polypeptide-k and oil isolated from seeds of Momordica charantia (bitter gourd) Ahmad, Zuraini Zamhuri, Khairul Faizi Yaacob, Azhar Chiong, Hoe Siong Selvarajah, Malarvili Ismail, Amin Abdullah, Muhammad Nazrul Hakim The amino acid and fatty acid composition of polypeptide k and oil isolated from the seeds of Momordica charantia was analysed. The analysis revealed polypeptide k contained 9 out of 11 essential amino acids, among a total of 18 types of amino acids. Glutamic acid, aspartic acid, arginine and glycine were the most abundant (17.08%, 9.71%, 9.50% and 8.90% of total amino acids, respectively). Fatty acid analysis showed unusually high amounts of C18-0 (stearic acid, 62.31% of total fatty acid). C18-1 (oleic acid) and C18-2 (linoleic acid) were the other major fatty acid detected (12.53% and 10.40%, respectively). The oil was devoid of the short fatty acids (C4-0 to C8-0). Polypeptide k and oil were also subjected to in vitro α-glucosidase and α-amylase inhibition assays. Both polypeptide k and seed oil showed potent inhibition of α-glucosidase enzyme (79.18% and 53.55% inhibition, respectively). α-Amylase was inhibited by 35.58% and 38.02%, respectively. Collectively, the in vitro assay strongly suggests that both polypeptide k and seed oil from Momordica charantia are potent potential hypoglycemic agents. MDPI 2012 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/77969/1/77969.pdf Ahmad, Zuraini and Zamhuri, Khairul Faizi and Yaacob, Azhar and Chiong, Hoe Siong and Selvarajah, Malarvili and Ismail, Amin and Abdullah, Muhammad Nazrul Hakim (2012) In vitro anti-diabetic activities and chemical analysis of polypeptide-k and oil isolated from seeds of Momordica charantia (bitter gourd). Molecules, 17 (8). pp. 9631-9640. ISSN 1420-3049 https://www.mdpi.com/1420-3049/17/8/9631 10.3390/molecules17089631 |
| spellingShingle | Ahmad, Zuraini Zamhuri, Khairul Faizi Yaacob, Azhar Chiong, Hoe Siong Selvarajah, Malarvili Ismail, Amin Abdullah, Muhammad Nazrul Hakim In vitro anti-diabetic activities and chemical analysis of polypeptide-k and oil isolated from seeds of Momordica charantia (bitter gourd) |
| title | In vitro anti-diabetic activities and chemical analysis of polypeptide-k and oil isolated from seeds of Momordica charantia (bitter gourd) |
| title_full | In vitro anti-diabetic activities and chemical analysis of polypeptide-k and oil isolated from seeds of Momordica charantia (bitter gourd) |
| title_fullStr | In vitro anti-diabetic activities and chemical analysis of polypeptide-k and oil isolated from seeds of Momordica charantia (bitter gourd) |
| title_full_unstemmed | In vitro anti-diabetic activities and chemical analysis of polypeptide-k and oil isolated from seeds of Momordica charantia (bitter gourd) |
| title_short | In vitro anti-diabetic activities and chemical analysis of polypeptide-k and oil isolated from seeds of Momordica charantia (bitter gourd) |
| title_sort | in vitro anti-diabetic activities and chemical analysis of polypeptide-k and oil isolated from seeds of momordica charantia (bitter gourd) |
| url | http://psasir.upm.edu.my/id/eprint/77969/ http://psasir.upm.edu.my/id/eprint/77969/ http://psasir.upm.edu.my/id/eprint/77969/ http://psasir.upm.edu.my/id/eprint/77969/1/77969.pdf |