Triterpene composition and bioactivities of Centella asiatica

Leaves of Centella asiatica (Centella) were analysed for their triterpene composition and bioactivity such as collagen enhancement, antioxidant, anticellulite and UV protection capacity properties. Triterpenes of Centella were measured using HPLC-PAD on an Excil ODS 5 mm (C18) column for the simulta...

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Main Authors: Hashim, Puziah, Sidek, Hamidah, Mohd Helan, Mohd Helme, Sabery, Aidawati, M. Palanisamy, Uma Devi, Adenan, Mohd Ilham
Format: Article
Language:English
Published: MDPI 2011
Online Access:http://psasir.upm.edu.my/id/eprint/77954/
http://psasir.upm.edu.my/id/eprint/77954/1/77954.pdf
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author Hashim, Puziah
Sidek, Hamidah
Mohd Helan, Mohd Helme
Sabery, Aidawati
M. Palanisamy, Uma Devi
Adenan, Mohd Ilham
author_facet Hashim, Puziah
Sidek, Hamidah
Mohd Helan, Mohd Helme
Sabery, Aidawati
M. Palanisamy, Uma Devi
Adenan, Mohd Ilham
author_sort Hashim, Puziah
building UPM Institutional Repository
collection Online Access
description Leaves of Centella asiatica (Centella) were analysed for their triterpene composition and bioactivity such as collagen enhancement, antioxidant, anticellulite and UV protection capacity properties. Triterpenes of Centella were measured using HPLC-PAD on an Excil ODS 5 mm (C18) column for the simultaneous determination of asiatic acid, madecassic acid, asiaticoside and madecassoside. Centella was found to contain significant amounts of madecassoside (3.10 ± 4.58 mg/mL) and asiaticoside (1.97 ± 2.65 mg/mL), but was low in asiatic and madecassic acid. The highest collagen synthesis was found at 50 mg/mL of Centella extracts. The antioxidant activity of Centella (84%) was compared to grape seed extract (83%) and Vitamin C (88%). Its lipolytic activity was observed by the release of glycerol (115.9 µmol/L) at 0.02% concentration. Centella extracts exhibited similar UV protection effect to OMC at 10% concentration. In view of these results, the potential application of Centella in food and pharmaceutical industries is now widely open.
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spelling upm-779542020-05-04T17:51:39Z http://psasir.upm.edu.my/id/eprint/77954/ Triterpene composition and bioactivities of Centella asiatica Hashim, Puziah Sidek, Hamidah Mohd Helan, Mohd Helme Sabery, Aidawati M. Palanisamy, Uma Devi Adenan, Mohd Ilham Leaves of Centella asiatica (Centella) were analysed for their triterpene composition and bioactivity such as collagen enhancement, antioxidant, anticellulite and UV protection capacity properties. Triterpenes of Centella were measured using HPLC-PAD on an Excil ODS 5 mm (C18) column for the simultaneous determination of asiatic acid, madecassic acid, asiaticoside and madecassoside. Centella was found to contain significant amounts of madecassoside (3.10 ± 4.58 mg/mL) and asiaticoside (1.97 ± 2.65 mg/mL), but was low in asiatic and madecassic acid. The highest collagen synthesis was found at 50 mg/mL of Centella extracts. The antioxidant activity of Centella (84%) was compared to grape seed extract (83%) and Vitamin C (88%). Its lipolytic activity was observed by the release of glycerol (115.9 µmol/L) at 0.02% concentration. Centella extracts exhibited similar UV protection effect to OMC at 10% concentration. In view of these results, the potential application of Centella in food and pharmaceutical industries is now widely open. MDPI 2011 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/77954/1/77954.pdf Hashim, Puziah and Sidek, Hamidah and Mohd Helan, Mohd Helme and Sabery, Aidawati and M. Palanisamy, Uma Devi and Adenan, Mohd Ilham (2011) Triterpene composition and bioactivities of Centella asiatica. Molecules, 16 (2). pp. 1310-1322. ISSN 1420-3049 https://www.mdpi.com/1420-3049/16/2/1310 10.3390/molecules16021310
spellingShingle Hashim, Puziah
Sidek, Hamidah
Mohd Helan, Mohd Helme
Sabery, Aidawati
M. Palanisamy, Uma Devi
Adenan, Mohd Ilham
Triterpene composition and bioactivities of Centella asiatica
title Triterpene composition and bioactivities of Centella asiatica
title_full Triterpene composition and bioactivities of Centella asiatica
title_fullStr Triterpene composition and bioactivities of Centella asiatica
title_full_unstemmed Triterpene composition and bioactivities of Centella asiatica
title_short Triterpene composition and bioactivities of Centella asiatica
title_sort triterpene composition and bioactivities of centella asiatica
url http://psasir.upm.edu.my/id/eprint/77954/
http://psasir.upm.edu.my/id/eprint/77954/
http://psasir.upm.edu.my/id/eprint/77954/
http://psasir.upm.edu.my/id/eprint/77954/1/77954.pdf