Lethal dose and clinical signs of Streptococcus agalactiae in Javanese medaka (Oryzias javanicus Bleeker 1854)
This study was conducted to determine the lethal dose and clinical signs of Streptococcus agalactiae in Javanese medaka. Javanese medaka was collected in Sungai Pelek, Sepang and brought to the laboratory for acclimatization. The fish were then challenged from 102 - 108 CFU/ml of S. agalactiae via i...
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| Format: | Conference or Workshop Item |
| Language: | English |
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Department of Biology, Faculty of Science, Universiti Putra Malaysia
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/77579/ http://psasir.upm.edu.my/id/eprint/77579/1/i-SIMBIOMAS%202016%2092.pdf |
| _version_ | 1848858287530835968 |
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| author | Mastor, Siti Suhaiba Azmai, Mohammad Noor Amal Ismail, Ahmad Saad, Mohd Zamri Nasruddin, Nurrul Shaqinah |
| author_facet | Mastor, Siti Suhaiba Azmai, Mohammad Noor Amal Ismail, Ahmad Saad, Mohd Zamri Nasruddin, Nurrul Shaqinah |
| author_sort | Mastor, Siti Suhaiba |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study was conducted to determine the lethal dose and clinical signs of Streptococcus agalactiae in Javanese medaka. Javanese medaka was collected in Sungai Pelek, Sepang and brought to the laboratory for acclimatization. The fish were then challenged from 102 - 108 CFU/ml of S. agalactiae via intraperitoneal injection. Mortalities and clinical signs were observed until 10 days of exposure, while the dead fish were collected for bacterial isolation and histological analyses. Lethal dose 50% (LD50) of S. agalactiae in Javanese medaka was determined at 6.5 × 103 CFU/mL. Most of the infected fish showing lethargy, erratic swimming, exophthalmia, opacity and necrosis at the injection site. The histopathological changes were mainly generalised congestion of the internal organs. Streptococcus agalactiae were successfully isolated from the dead fish. Results showed that Javanese medaka was susceptible towards S. agalactiae infection and could be a potential alternative organism for study of streptococcocis in fish. |
| first_indexed | 2025-11-15T12:11:03Z |
| format | Conference or Workshop Item |
| id | upm-77579 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:11:03Z |
| publishDate | 2016 |
| publisher | Department of Biology, Faculty of Science, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-775792020-04-15T16:35:07Z http://psasir.upm.edu.my/id/eprint/77579/ Lethal dose and clinical signs of Streptococcus agalactiae in Javanese medaka (Oryzias javanicus Bleeker 1854) Mastor, Siti Suhaiba Azmai, Mohammad Noor Amal Ismail, Ahmad Saad, Mohd Zamri Nasruddin, Nurrul Shaqinah This study was conducted to determine the lethal dose and clinical signs of Streptococcus agalactiae in Javanese medaka. Javanese medaka was collected in Sungai Pelek, Sepang and brought to the laboratory for acclimatization. The fish were then challenged from 102 - 108 CFU/ml of S. agalactiae via intraperitoneal injection. Mortalities and clinical signs were observed until 10 days of exposure, while the dead fish were collected for bacterial isolation and histological analyses. Lethal dose 50% (LD50) of S. agalactiae in Javanese medaka was determined at 6.5 × 103 CFU/mL. Most of the infected fish showing lethargy, erratic swimming, exophthalmia, opacity and necrosis at the injection site. The histopathological changes were mainly generalised congestion of the internal organs. Streptococcus agalactiae were successfully isolated from the dead fish. Results showed that Javanese medaka was susceptible towards S. agalactiae infection and could be a potential alternative organism for study of streptococcocis in fish. Department of Biology, Faculty of Science, Universiti Putra Malaysia 2016 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/77579/1/i-SIMBIOMAS%202016%2092.pdf Mastor, Siti Suhaiba and Azmai, Mohammad Noor Amal and Ismail, Ahmad and Saad, Mohd Zamri and Nasruddin, Nurrul Shaqinah (2016) Lethal dose and clinical signs of Streptococcus agalactiae in Javanese medaka (Oryzias javanicus Bleeker 1854). In: Malaysia International Biology Symposium 2016, 26-27 Oct. 2016, PICC, Putrajaya, Malaysia. (p. 92). |
| spellingShingle | Mastor, Siti Suhaiba Azmai, Mohammad Noor Amal Ismail, Ahmad Saad, Mohd Zamri Nasruddin, Nurrul Shaqinah Lethal dose and clinical signs of Streptococcus agalactiae in Javanese medaka (Oryzias javanicus Bleeker 1854) |
| title | Lethal dose and clinical signs of Streptococcus agalactiae in Javanese medaka (Oryzias javanicus Bleeker 1854) |
| title_full | Lethal dose and clinical signs of Streptococcus agalactiae in Javanese medaka (Oryzias javanicus Bleeker 1854) |
| title_fullStr | Lethal dose and clinical signs of Streptococcus agalactiae in Javanese medaka (Oryzias javanicus Bleeker 1854) |
| title_full_unstemmed | Lethal dose and clinical signs of Streptococcus agalactiae in Javanese medaka (Oryzias javanicus Bleeker 1854) |
| title_short | Lethal dose and clinical signs of Streptococcus agalactiae in Javanese medaka (Oryzias javanicus Bleeker 1854) |
| title_sort | lethal dose and clinical signs of streptococcus agalactiae in javanese medaka (oryzias javanicus bleeker 1854) |
| url | http://psasir.upm.edu.my/id/eprint/77579/ http://psasir.upm.edu.my/id/eprint/77579/1/i-SIMBIOMAS%202016%2092.pdf |