Cross-species microsatellite amplification of river terrapin (Batagur affinis)
Batagur affinis (river terrapin or “tuntung”) is one of the world’s most critically endangered species. It is distributed across Sumatera, Malaysia, Thailand and Cambodia. Its population number is declining in the wild because of habitat destructions and it economic value (i.e. exotic delicacies for...
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| Format: | Conference or Workshop Item |
| Language: | English |
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Department of Biology, Faculty of Science, Universiti Putra Malaysia
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/77574/ http://psasir.upm.edu.my/id/eprint/77574/1/i-SIMBIOMAS%202016%2081.pdf |
| _version_ | 1848858286039760896 |
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| author | Ismail, Nurul Aini Ab Ghani, Nurul Izza Ismail, Ahmad |
| author_facet | Ismail, Nurul Aini Ab Ghani, Nurul Izza Ismail, Ahmad |
| author_sort | Ismail, Nurul Aini |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Batagur affinis (river terrapin or “tuntung”) is one of the world’s most critically endangered species. It is distributed across Sumatera, Malaysia, Thailand and Cambodia. Its population number is declining in the wild because of habitat destructions and it economic value (i.e. exotic delicacies for eggs and meat). Hence, many reintroduction efforts had been done. Yet, the wild populations are not improving. One of the possible reasons behind this failure is possibly due to low genetic diversity in this species. Thus, population genetics study should be conducted. In this preliminary population genetics study, a total of 33 microsatellite primers from other freshwater terrapin (i.e. B. trivittata and Podocnemis unifilis) were screened and optimised for 30 samples of river terrapin from Kemaman, Terengganu using touchdown protocol and followed by gradient protocol. Among those 33 primers, 23 primers were able to cross-amplify with B. affinis and optimised for annealing temperature (51 – 70°C). Those 23 primers will be used for future population genetics study using samples from both Bota Kanan, Perak and Kemaman, Terengganu. |
| first_indexed | 2025-11-15T12:11:01Z |
| format | Conference or Workshop Item |
| id | upm-77574 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:11:01Z |
| publishDate | 2016 |
| publisher | Department of Biology, Faculty of Science, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-775742020-04-15T16:35:47Z http://psasir.upm.edu.my/id/eprint/77574/ Cross-species microsatellite amplification of river terrapin (Batagur affinis) Ismail, Nurul Aini Ab Ghani, Nurul Izza Ismail, Ahmad Batagur affinis (river terrapin or “tuntung”) is one of the world’s most critically endangered species. It is distributed across Sumatera, Malaysia, Thailand and Cambodia. Its population number is declining in the wild because of habitat destructions and it economic value (i.e. exotic delicacies for eggs and meat). Hence, many reintroduction efforts had been done. Yet, the wild populations are not improving. One of the possible reasons behind this failure is possibly due to low genetic diversity in this species. Thus, population genetics study should be conducted. In this preliminary population genetics study, a total of 33 microsatellite primers from other freshwater terrapin (i.e. B. trivittata and Podocnemis unifilis) were screened and optimised for 30 samples of river terrapin from Kemaman, Terengganu using touchdown protocol and followed by gradient protocol. Among those 33 primers, 23 primers were able to cross-amplify with B. affinis and optimised for annealing temperature (51 – 70°C). Those 23 primers will be used for future population genetics study using samples from both Bota Kanan, Perak and Kemaman, Terengganu. Department of Biology, Faculty of Science, Universiti Putra Malaysia 2016 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/77574/1/i-SIMBIOMAS%202016%2081.pdf Ismail, Nurul Aini and Ab Ghani, Nurul Izza and Ismail, Ahmad (2016) Cross-species microsatellite amplification of river terrapin (Batagur affinis). In: Malaysia International Biology Symposium 2016, 26-27 Oct. 2016, PICC, Putrajaya, Malaysia. (p. 81). |
| spellingShingle | Ismail, Nurul Aini Ab Ghani, Nurul Izza Ismail, Ahmad Cross-species microsatellite amplification of river terrapin (Batagur affinis) |
| title | Cross-species microsatellite amplification of river terrapin (Batagur affinis) |
| title_full | Cross-species microsatellite amplification of river terrapin (Batagur affinis) |
| title_fullStr | Cross-species microsatellite amplification of river terrapin (Batagur affinis) |
| title_full_unstemmed | Cross-species microsatellite amplification of river terrapin (Batagur affinis) |
| title_short | Cross-species microsatellite amplification of river terrapin (Batagur affinis) |
| title_sort | cross-species microsatellite amplification of river terrapin (batagur affinis) |
| url | http://psasir.upm.edu.my/id/eprint/77574/ http://psasir.upm.edu.my/id/eprint/77574/1/i-SIMBIOMAS%202016%2081.pdf |