Resistance capability of indigenous and non-indigenous bacteria species on 3,4-dichoroaniline

3,4- dichloroaniline (3,4-DCA) is commonly be used in the chemical industry and it is also a by-product of aniline-derived herbicides such as diuron, linuron and propanil. However, 3,4-DCA exhibits higher toxicity than its parent compound and persistent in soils, water and groundwater. The aim of st...

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Main Authors: Mohd Zaini, N. A., Hanapiah, Munirah, Mustafa, Muskhazli, Zulkifli, Syaizwan Zahmir, Mohamat Yusuff, Ferdius @ Ferdaus, Ismail, Ahmad
Format: Conference or Workshop Item
Language:English
Published: Department of Biology, Faculty of Science, Universiti Putra Malaysia 2016
Online Access:http://psasir.upm.edu.my/id/eprint/77559/
http://psasir.upm.edu.my/id/eprint/77559/1/i-SIMBIOMAS%202016%2062.pdf
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author Mohd Zaini, N. A.
Hanapiah, Munirah
Mustafa, Muskhazli
Zulkifli, Syaizwan Zahmir
Mohamat Yusuff, Ferdius @ Ferdaus
Ismail, Ahmad
author_facet Mohd Zaini, N. A.
Hanapiah, Munirah
Mustafa, Muskhazli
Zulkifli, Syaizwan Zahmir
Mohamat Yusuff, Ferdius @ Ferdaus
Ismail, Ahmad
author_sort Mohd Zaini, N. A.
building UPM Institutional Repository
collection Online Access
description 3,4- dichloroaniline (3,4-DCA) is commonly be used in the chemical industry and it is also a by-product of aniline-derived herbicides such as diuron, linuron and propanil. However, 3,4-DCA exhibits higher toxicity than its parent compound and persistent in soils, water and groundwater. The aim of study was to investigate three TBT-resistant bacteria which are Klebsiella sp., Acinetobacter sp., Citrobacter sp. to evaluate their resistancy towards 3,4-DCA. Pseudomonas sp. isolated from non-TBT-polluted area was used as a positive control. Respiratory inhibitory activity of 3,4-DCA on three bacteria strains were assessed by employing MTT-bioassay. Results showed, Citrobacter sp. is the most tolerable bacteria amongst three strains with the IC50 value of 7.83 mg/L followed by Acinetobacter sp. (7.55 mg/L), Klebsiella sp. (7.09 mg/L) and Pseudomonas sp. (7.02 mg/L). Due to that, potential TBT-degrading bacteria, Klebsiella sp. is not preferable bacteria to degrade or utilize 3,4-DCA mainly because of the structure and resistancy bacteria itself. Thus, future studies are recommended in order to remediate this highly persistant compound in solving environmental problems associated contamination.
first_indexed 2025-11-15T12:10:58Z
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institution Universiti Putra Malaysia
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language English
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publisher Department of Biology, Faculty of Science, Universiti Putra Malaysia
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spelling upm-775592020-04-15T16:41:39Z http://psasir.upm.edu.my/id/eprint/77559/ Resistance capability of indigenous and non-indigenous bacteria species on 3,4-dichoroaniline Mohd Zaini, N. A. Hanapiah, Munirah Mustafa, Muskhazli Zulkifli, Syaizwan Zahmir Mohamat Yusuff, Ferdius @ Ferdaus Ismail, Ahmad 3,4- dichloroaniline (3,4-DCA) is commonly be used in the chemical industry and it is also a by-product of aniline-derived herbicides such as diuron, linuron and propanil. However, 3,4-DCA exhibits higher toxicity than its parent compound and persistent in soils, water and groundwater. The aim of study was to investigate three TBT-resistant bacteria which are Klebsiella sp., Acinetobacter sp., Citrobacter sp. to evaluate their resistancy towards 3,4-DCA. Pseudomonas sp. isolated from non-TBT-polluted area was used as a positive control. Respiratory inhibitory activity of 3,4-DCA on three bacteria strains were assessed by employing MTT-bioassay. Results showed, Citrobacter sp. is the most tolerable bacteria amongst three strains with the IC50 value of 7.83 mg/L followed by Acinetobacter sp. (7.55 mg/L), Klebsiella sp. (7.09 mg/L) and Pseudomonas sp. (7.02 mg/L). Due to that, potential TBT-degrading bacteria, Klebsiella sp. is not preferable bacteria to degrade or utilize 3,4-DCA mainly because of the structure and resistancy bacteria itself. Thus, future studies are recommended in order to remediate this highly persistant compound in solving environmental problems associated contamination. Department of Biology, Faculty of Science, Universiti Putra Malaysia 2016 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/77559/1/i-SIMBIOMAS%202016%2062.pdf Mohd Zaini, N. A. and Hanapiah, Munirah and Mustafa, Muskhazli and Zulkifli, Syaizwan Zahmir and Mohamat Yusuff, Ferdius @ Ferdaus and Ismail, Ahmad (2016) Resistance capability of indigenous and non-indigenous bacteria species on 3,4-dichoroaniline. In: Malaysia International Biology Symposium 2016, 26-27 Oct. 2016, PICC, Putrajaya, Malaysia. (p. 62).
spellingShingle Mohd Zaini, N. A.
Hanapiah, Munirah
Mustafa, Muskhazli
Zulkifli, Syaizwan Zahmir
Mohamat Yusuff, Ferdius @ Ferdaus
Ismail, Ahmad
Resistance capability of indigenous and non-indigenous bacteria species on 3,4-dichoroaniline
title Resistance capability of indigenous and non-indigenous bacteria species on 3,4-dichoroaniline
title_full Resistance capability of indigenous and non-indigenous bacteria species on 3,4-dichoroaniline
title_fullStr Resistance capability of indigenous and non-indigenous bacteria species on 3,4-dichoroaniline
title_full_unstemmed Resistance capability of indigenous and non-indigenous bacteria species on 3,4-dichoroaniline
title_short Resistance capability of indigenous and non-indigenous bacteria species on 3,4-dichoroaniline
title_sort resistance capability of indigenous and non-indigenous bacteria species on 3,4-dichoroaniline
url http://psasir.upm.edu.my/id/eprint/77559/
http://psasir.upm.edu.my/id/eprint/77559/1/i-SIMBIOMAS%202016%2062.pdf