Antibacterial activity of garlic extracts on fish pathogenic bacteria

Aqueous and methanol extracts of Allium sativum (clove and peel) were investigated for its in vitro antibacterial properties against four marine pathogens, Aeromonas hydrophila, Vibrio anguillarum, Vibrio alginolyticus, and Vibrio harveyi. Antibacterial activity of different concentrations of aqueou...

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Main Authors: Rosland, Nur Natasya Ain, Nordin, Eirna Liza, Mohd Yaminudin, Nur Jasmin, Abd Karim, Murni Marlina
Format: Conference or Workshop Item
Language:English
Published: Department of Biology, Faculty of Science, Universiti Putra Malaysia 2016
Online Access:http://psasir.upm.edu.my/id/eprint/77502/
http://psasir.upm.edu.my/id/eprint/77502/1/i-SIMBIOMAS%202016%2044.pdf
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author Rosland, Nur Natasya Ain
Nordin, Eirna Liza
Mohd Yaminudin, Nur Jasmin
Abd Karim, Murni Marlina
author_facet Rosland, Nur Natasya Ain
Nordin, Eirna Liza
Mohd Yaminudin, Nur Jasmin
Abd Karim, Murni Marlina
author_sort Rosland, Nur Natasya Ain
building UPM Institutional Repository
collection Online Access
description Aqueous and methanol extracts of Allium sativum (clove and peel) were investigated for its in vitro antibacterial properties against four marine pathogens, Aeromonas hydrophila, Vibrio anguillarum, Vibrio alginolyticus, and Vibrio harveyi. Antibacterial activity of different concentrations of aqueous and methanol garlic extracts were evaluated based on the inhibition zone using disc-diffusion method, minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values. The aqueous extract of A. sativum (clove and peel) had no antibacterial effect against the pathogenic bacteria tested whereas the clove extract of methanol had inhibitory effects on the growth of all pathogenic bacteria tested. The maximum zone of inhibition was observed in A. hydrophila and the minimum was observed in V. anguillarum and V. harveyi. The MIC and MBC values revealed that A. hydrophila was able to be inhibited by supernatant extract of clove at lower concentration (0.6 mg/ml) while other pathogens are inhibited at slightly higher concentration. This study suggests that clove extract of A. sativum has the potential to be used as phytobiotics in controlling the growth of marine pathogens.
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format Conference or Workshop Item
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institution Universiti Putra Malaysia
institution_category Local University
language English
last_indexed 2025-11-15T12:10:43Z
publishDate 2016
publisher Department of Biology, Faculty of Science, Universiti Putra Malaysia
recordtype eprints
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spelling upm-775022020-04-15T16:43:25Z http://psasir.upm.edu.my/id/eprint/77502/ Antibacterial activity of garlic extracts on fish pathogenic bacteria Rosland, Nur Natasya Ain Nordin, Eirna Liza Mohd Yaminudin, Nur Jasmin Abd Karim, Murni Marlina Aqueous and methanol extracts of Allium sativum (clove and peel) were investigated for its in vitro antibacterial properties against four marine pathogens, Aeromonas hydrophila, Vibrio anguillarum, Vibrio alginolyticus, and Vibrio harveyi. Antibacterial activity of different concentrations of aqueous and methanol garlic extracts were evaluated based on the inhibition zone using disc-diffusion method, minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values. The aqueous extract of A. sativum (clove and peel) had no antibacterial effect against the pathogenic bacteria tested whereas the clove extract of methanol had inhibitory effects on the growth of all pathogenic bacteria tested. The maximum zone of inhibition was observed in A. hydrophila and the minimum was observed in V. anguillarum and V. harveyi. The MIC and MBC values revealed that A. hydrophila was able to be inhibited by supernatant extract of clove at lower concentration (0.6 mg/ml) while other pathogens are inhibited at slightly higher concentration. This study suggests that clove extract of A. sativum has the potential to be used as phytobiotics in controlling the growth of marine pathogens. Department of Biology, Faculty of Science, Universiti Putra Malaysia 2016 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/77502/1/i-SIMBIOMAS%202016%2044.pdf Rosland, Nur Natasya Ain and Nordin, Eirna Liza and Mohd Yaminudin, Nur Jasmin and Abd Karim, Murni Marlina (2016) Antibacterial activity of garlic extracts on fish pathogenic bacteria. In: Malaysia International Biology Symposium 2016, 26-27 Oct. 2016, PICC, Putrajaya, Malaysia. (p. 44).
spellingShingle Rosland, Nur Natasya Ain
Nordin, Eirna Liza
Mohd Yaminudin, Nur Jasmin
Abd Karim, Murni Marlina
Antibacterial activity of garlic extracts on fish pathogenic bacteria
title Antibacterial activity of garlic extracts on fish pathogenic bacteria
title_full Antibacterial activity of garlic extracts on fish pathogenic bacteria
title_fullStr Antibacterial activity of garlic extracts on fish pathogenic bacteria
title_full_unstemmed Antibacterial activity of garlic extracts on fish pathogenic bacteria
title_short Antibacterial activity of garlic extracts on fish pathogenic bacteria
title_sort antibacterial activity of garlic extracts on fish pathogenic bacteria
url http://psasir.upm.edu.my/id/eprint/77502/
http://psasir.upm.edu.my/id/eprint/77502/1/i-SIMBIOMAS%202016%2044.pdf