Isolation, molecular identification and biodegradation capacities of indigenous hydrocarbon-degrading bacteria from tarball at Terengganu beach, Malaysia
In this study, we isolated four indigenous hydrocarbon-degrading bacteria from tarball found in Rhu Sepuluh beach, Terengganu, Malaysia. These bacteria were identified based on their physiological characteristic and 16S rRNA gene sequence analysis, and they showed 99% similarity with Pseudomonas stu...
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| Format: | Conference or Workshop Item |
| Language: | English |
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Department of Biology, Faculty of Science, Universiti Putra Malaysia
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/77481/ http://psasir.upm.edu.my/id/eprint/77481/1/i-SIMBIOMAS%202016%2024.pdf |
| _version_ | 1848858261023883264 |
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| author | Nkem, Bruno Martins Halimoon, Normala Md. Yusoff, Fatimah Wan Johari, Wan Lutfi Zakaria, Mohamad Pauzi Medipally, Srikanth Reddy Kannan, Narayanan |
| author_facet | Nkem, Bruno Martins Halimoon, Normala Md. Yusoff, Fatimah Wan Johari, Wan Lutfi Zakaria, Mohamad Pauzi Medipally, Srikanth Reddy Kannan, Narayanan |
| author_sort | Nkem, Bruno Martins |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In this study, we isolated four indigenous hydrocarbon-degrading bacteria from tarball found in Rhu Sepuluh beach, Terengganu, Malaysia. These bacteria were identified based on their physiological characteristic and 16S rRNA gene sequence analysis, and they showed 99% similarity with Pseudomonas stutzeri strain A1501, Pseudomonas balearica strain SP1402, Cellulosimicrobium cellulans DSM 43879 and Acinetobacter baumannii ATCC 19606 respectively. Their hydrocarbon-degrading capabilities were tested using diesel-oil as sole carbon source. Results analysed using GC-MS, showed diesel-oil alkanes were degraded an average 30.5%, 16.4%, 64.4% and 58.1% with medium optical density reaching 1.172, 1.503, 0.967 and 1.515 by Pseudomonas stutzeri, Pseudomonas balearica, Cellulosimicrobium cellulans and Acinetobacter baumannii respectively in minimal salt media at 32oC for 10 days. Individual diesel-oil alkanes were degraded between 19.7% - 49.2% (P. stutzeri), 2.5% to 27.5% (P. balearica), 10% - 95.4% (C. cellulans) and 0.2% - 95.9% (A. baumannii). All strains utilized diesel-oil for growth. The study suggests all strains are part of indigenous hydrocarbon-degrading bacteria in tarball with potential for bioremediation of oil-polluted marine environment. |
| first_indexed | 2025-11-15T12:10:38Z |
| format | Conference or Workshop Item |
| id | upm-77481 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:10:38Z |
| publishDate | 2016 |
| publisher | Department of Biology, Faculty of Science, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-774812020-04-15T16:45:37Z http://psasir.upm.edu.my/id/eprint/77481/ Isolation, molecular identification and biodegradation capacities of indigenous hydrocarbon-degrading bacteria from tarball at Terengganu beach, Malaysia Nkem, Bruno Martins Halimoon, Normala Md. Yusoff, Fatimah Wan Johari, Wan Lutfi Zakaria, Mohamad Pauzi Medipally, Srikanth Reddy Kannan, Narayanan In this study, we isolated four indigenous hydrocarbon-degrading bacteria from tarball found in Rhu Sepuluh beach, Terengganu, Malaysia. These bacteria were identified based on their physiological characteristic and 16S rRNA gene sequence analysis, and they showed 99% similarity with Pseudomonas stutzeri strain A1501, Pseudomonas balearica strain SP1402, Cellulosimicrobium cellulans DSM 43879 and Acinetobacter baumannii ATCC 19606 respectively. Their hydrocarbon-degrading capabilities were tested using diesel-oil as sole carbon source. Results analysed using GC-MS, showed diesel-oil alkanes were degraded an average 30.5%, 16.4%, 64.4% and 58.1% with medium optical density reaching 1.172, 1.503, 0.967 and 1.515 by Pseudomonas stutzeri, Pseudomonas balearica, Cellulosimicrobium cellulans and Acinetobacter baumannii respectively in minimal salt media at 32oC for 10 days. Individual diesel-oil alkanes were degraded between 19.7% - 49.2% (P. stutzeri), 2.5% to 27.5% (P. balearica), 10% - 95.4% (C. cellulans) and 0.2% - 95.9% (A. baumannii). All strains utilized diesel-oil for growth. The study suggests all strains are part of indigenous hydrocarbon-degrading bacteria in tarball with potential for bioremediation of oil-polluted marine environment. Department of Biology, Faculty of Science, Universiti Putra Malaysia 2016 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/77481/1/i-SIMBIOMAS%202016%2024.pdf Nkem, Bruno Martins and Halimoon, Normala and Md. Yusoff, Fatimah and Wan Johari, Wan Lutfi and Zakaria, Mohamad Pauzi and Medipally, Srikanth Reddy and Kannan, Narayanan (2016) Isolation, molecular identification and biodegradation capacities of indigenous hydrocarbon-degrading bacteria from tarball at Terengganu beach, Malaysia. In: Malaysia International Biology Symposium 2016, 26-27 Oct. 2016, PICC, Putrajaya, Malaysia. (p. 24). |
| spellingShingle | Nkem, Bruno Martins Halimoon, Normala Md. Yusoff, Fatimah Wan Johari, Wan Lutfi Zakaria, Mohamad Pauzi Medipally, Srikanth Reddy Kannan, Narayanan Isolation, molecular identification and biodegradation capacities of indigenous hydrocarbon-degrading bacteria from tarball at Terengganu beach, Malaysia |
| title | Isolation, molecular identification and biodegradation capacities of indigenous hydrocarbon-degrading bacteria from tarball at Terengganu beach, Malaysia |
| title_full | Isolation, molecular identification and biodegradation capacities of indigenous hydrocarbon-degrading bacteria from tarball at Terengganu beach, Malaysia |
| title_fullStr | Isolation, molecular identification and biodegradation capacities of indigenous hydrocarbon-degrading bacteria from tarball at Terengganu beach, Malaysia |
| title_full_unstemmed | Isolation, molecular identification and biodegradation capacities of indigenous hydrocarbon-degrading bacteria from tarball at Terengganu beach, Malaysia |
| title_short | Isolation, molecular identification and biodegradation capacities of indigenous hydrocarbon-degrading bacteria from tarball at Terengganu beach, Malaysia |
| title_sort | isolation, molecular identification and biodegradation capacities of indigenous hydrocarbon-degrading bacteria from tarball at terengganu beach, malaysia |
| url | http://psasir.upm.edu.my/id/eprint/77481/ http://psasir.upm.edu.my/id/eprint/77481/1/i-SIMBIOMAS%202016%2024.pdf |