In-vitro antifungal activity of Vernonia amygdalina against Botrytis cinerea in tomato
Grey mold disease caused by Botrytis cinerea is the most severe disease in tomato and known to be a limiting factor in tomato production worldwide. Synthetic fungicide is widely used to control this pathogen. However, it posed detrimental effects to the environment and human health as well as increa...
| Main Authors: | , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
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Faculty of Agriculture, Universiti Putra Malaysia
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/77371/ http://psasir.upm.edu.my/id/eprint/77371/1/8TH%20IAC%20%E2%80%93%206TH%20ISFA%202018-50.pdf |
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| author | Yusoff, Siti Fairuz Tengku Muda Mohamed, Mahmud Haron, Farah Farhanah Ismail, Siti Izera Sakimin, Siti Zaharah |
| author_facet | Yusoff, Siti Fairuz Tengku Muda Mohamed, Mahmud Haron, Farah Farhanah Ismail, Siti Izera Sakimin, Siti Zaharah |
| author_sort | Yusoff, Siti Fairuz |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Grey mold disease caused by Botrytis cinerea is the most severe disease in tomato and known to be a limiting factor in tomato production worldwide. Synthetic fungicide is widely used to control this pathogen. However, it posed detrimental effects to the environment and human health as well as increasing resistance in B. cinerea. Vernonia amygdalina leaf extracts possess antifungal properties that are useful for the development of safer alternatives. Therefore, the aim of this study was to evaluate in-vitro antifungal activities of V. amygdalina extract against B. cinerea in tomato. The powdered leaves of V. amygdalina were sequentially extracted with organic solvents; (1) hexane, (2) dicholoromethane, (3) methanol, and water. In vitro antifungal test was conducted using poison agar technique. Among the crude extract, the effectiveness against B. cinerea as follows, dichloromethane > methanol > aqueous > hexane. Dichoromethane crude extract shown highly significant in PIRG and the antifungal activities were increased with the increasing of concentration level. The SEM observation proved that the treatment might alter the fungus morphology which lead to fungal growth inhibition. |
| first_indexed | 2025-11-15T12:10:09Z |
| format | Conference or Workshop Item |
| id | upm-77371 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:10:09Z |
| publishDate | 2018 |
| publisher | Faculty of Agriculture, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-773712020-03-05T06:28:53Z http://psasir.upm.edu.my/id/eprint/77371/ In-vitro antifungal activity of Vernonia amygdalina against Botrytis cinerea in tomato Yusoff, Siti Fairuz Tengku Muda Mohamed, Mahmud Haron, Farah Farhanah Ismail, Siti Izera Sakimin, Siti Zaharah Grey mold disease caused by Botrytis cinerea is the most severe disease in tomato and known to be a limiting factor in tomato production worldwide. Synthetic fungicide is widely used to control this pathogen. However, it posed detrimental effects to the environment and human health as well as increasing resistance in B. cinerea. Vernonia amygdalina leaf extracts possess antifungal properties that are useful for the development of safer alternatives. Therefore, the aim of this study was to evaluate in-vitro antifungal activities of V. amygdalina extract against B. cinerea in tomato. The powdered leaves of V. amygdalina were sequentially extracted with organic solvents; (1) hexane, (2) dicholoromethane, (3) methanol, and water. In vitro antifungal test was conducted using poison agar technique. Among the crude extract, the effectiveness against B. cinerea as follows, dichloromethane > methanol > aqueous > hexane. Dichoromethane crude extract shown highly significant in PIRG and the antifungal activities were increased with the increasing of concentration level. The SEM observation proved that the treatment might alter the fungus morphology which lead to fungal growth inhibition. Faculty of Agriculture, Universiti Putra Malaysia 2018 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/77371/1/8TH%20IAC%20%E2%80%93%206TH%20ISFA%202018-50.pdf Yusoff, Siti Fairuz and Tengku Muda Mohamed, Mahmud and Haron, Farah Farhanah and Ismail, Siti Izera and Sakimin, Siti Zaharah (2018) In-vitro antifungal activity of Vernonia amygdalina against Botrytis cinerea in tomato. In: Joint Symposium of the 8th International Agriculture Congress 2018 and 6th International Symposium for Food & Agriculture 2018 (8th IAC – 6th ISFA 2018), 13-15 Nov. 2018, Auditorium Rashdan Baba, TNCPI Building, Universiti Putra Malaysia. (pp. 248-252). |
| spellingShingle | Yusoff, Siti Fairuz Tengku Muda Mohamed, Mahmud Haron, Farah Farhanah Ismail, Siti Izera Sakimin, Siti Zaharah In-vitro antifungal activity of Vernonia amygdalina against Botrytis cinerea in tomato |
| title | In-vitro antifungal activity of Vernonia amygdalina against Botrytis cinerea in tomato |
| title_full | In-vitro antifungal activity of Vernonia amygdalina against Botrytis cinerea in tomato |
| title_fullStr | In-vitro antifungal activity of Vernonia amygdalina against Botrytis cinerea in tomato |
| title_full_unstemmed | In-vitro antifungal activity of Vernonia amygdalina against Botrytis cinerea in tomato |
| title_short | In-vitro antifungal activity of Vernonia amygdalina against Botrytis cinerea in tomato |
| title_sort | in-vitro antifungal activity of vernonia amygdalina against botrytis cinerea in tomato |
| url | http://psasir.upm.edu.my/id/eprint/77371/ http://psasir.upm.edu.my/id/eprint/77371/1/8TH%20IAC%20%E2%80%93%206TH%20ISFA%202018-50.pdf |