Bacterial quorum sensing degrader from biofloc and freshwater prawn, Macrobrachium rosenbergii associated with green microalgae Chlorella sp.
Quorum sensing is a cell-to-cell communication using signal molecules for the regulation virulence genes expression in bacteria. Interference with quorum sensing using QS degrader strains is currently being investigated to fight bacterial diseases. In this study, two potential strains of quorum sens...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Faculty of Agriculture, Universiti Putra Malaysia
2018
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/77355/ http://psasir.upm.edu.my/id/eprint/77355/1/8TH%20IAC%20%E2%80%93%206TH%20ISFA%202018-36.pdf |
| _version_ | 1848858226372640768 |
|---|---|
| author | G., Nurarina H., Aini Ying, L. H. Ahmad, I. A. Rahim, A. Md Yasin, Ina Salwany Mohd Ikhsan, Natrah Fatin |
| author_facet | G., Nurarina H., Aini Ying, L. H. Ahmad, I. A. Rahim, A. Md Yasin, Ina Salwany Mohd Ikhsan, Natrah Fatin |
| author_sort | G., Nurarina |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Quorum sensing is a cell-to-cell communication using signal molecules for the regulation virulence genes expression in bacteria. Interference with quorum sensing using QS degrader strains is currently being investigated to fight bacterial diseases. In this study, two potential strains of quorum sensing (QS) degrader were successfully isolated from biofloc and giant freshwater prawn, Macrobrachium rosenbergii. The strains were identified as Bacillus subtilis, BP-BFT/1b and Bacillus cereus, BP-MBRG/1b. Meanwhile, Chlorella sp. was made axenic using 13 to 500 ppm of different antibiotic mixtures (chloramphenicol, tetracycline, enrofloxacin, kanamycin, and neomycin) in which the interaction of the algae with different QS degrader strains, different microbial communities and pathogenic bacterium Aeromonas hydrophila were investigated. Growth of Chlorella sp. added with Bacillus subtilis, BP-BFT/1b was higher compared to Bacillus cereus, BP-MBRG/1b. The results showed that the growth of axenic Chlorella sp. was improved in the presence of bacteria, including the bacterial QS degraders. Hence, there is a potential to incorporate both microorganisms in aquaculture systems to overcome invasion of pathogenic bacteria through bacterial QS degrading mechanism. |
| first_indexed | 2025-11-15T12:10:05Z |
| format | Conference or Workshop Item |
| id | upm-77355 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:10:05Z |
| publishDate | 2018 |
| publisher | Faculty of Agriculture, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-773552020-03-05T06:27:14Z http://psasir.upm.edu.my/id/eprint/77355/ Bacterial quorum sensing degrader from biofloc and freshwater prawn, Macrobrachium rosenbergii associated with green microalgae Chlorella sp. G., Nurarina H., Aini Ying, L. H. Ahmad, I. A. Rahim, A. Md Yasin, Ina Salwany Mohd Ikhsan, Natrah Fatin Quorum sensing is a cell-to-cell communication using signal molecules for the regulation virulence genes expression in bacteria. Interference with quorum sensing using QS degrader strains is currently being investigated to fight bacterial diseases. In this study, two potential strains of quorum sensing (QS) degrader were successfully isolated from biofloc and giant freshwater prawn, Macrobrachium rosenbergii. The strains were identified as Bacillus subtilis, BP-BFT/1b and Bacillus cereus, BP-MBRG/1b. Meanwhile, Chlorella sp. was made axenic using 13 to 500 ppm of different antibiotic mixtures (chloramphenicol, tetracycline, enrofloxacin, kanamycin, and neomycin) in which the interaction of the algae with different QS degrader strains, different microbial communities and pathogenic bacterium Aeromonas hydrophila were investigated. Growth of Chlorella sp. added with Bacillus subtilis, BP-BFT/1b was higher compared to Bacillus cereus, BP-MBRG/1b. The results showed that the growth of axenic Chlorella sp. was improved in the presence of bacteria, including the bacterial QS degraders. Hence, there is a potential to incorporate both microorganisms in aquaculture systems to overcome invasion of pathogenic bacteria through bacterial QS degrading mechanism. Faculty of Agriculture, Universiti Putra Malaysia 2018 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/77355/1/8TH%20IAC%20%E2%80%93%206TH%20ISFA%202018-36.pdf G., Nurarina and H., Aini and Ying, L. H. and Ahmad, I. and A. Rahim, A. and Md Yasin, Ina Salwany and Mohd Ikhsan, Natrah Fatin (2018) Bacterial quorum sensing degrader from biofloc and freshwater prawn, Macrobrachium rosenbergii associated with green microalgae Chlorella sp. In: Joint Symposium of the 8th International Agriculture Congress 2018 and 6th International Symposium for Food & Agriculture 2018 (8th IAC – 6th ISFA 2018), 13-15 Nov. 2018, Auditorium Rashdan Baba, TNCPI Building, Universiti Putra Malaysia. (pp. 176-183). |
| spellingShingle | G., Nurarina H., Aini Ying, L. H. Ahmad, I. A. Rahim, A. Md Yasin, Ina Salwany Mohd Ikhsan, Natrah Fatin Bacterial quorum sensing degrader from biofloc and freshwater prawn, Macrobrachium rosenbergii associated with green microalgae Chlorella sp. |
| title | Bacterial quorum sensing degrader from biofloc and freshwater prawn, Macrobrachium rosenbergii associated with green microalgae Chlorella sp. |
| title_full | Bacterial quorum sensing degrader from biofloc and freshwater prawn, Macrobrachium rosenbergii associated with green microalgae Chlorella sp. |
| title_fullStr | Bacterial quorum sensing degrader from biofloc and freshwater prawn, Macrobrachium rosenbergii associated with green microalgae Chlorella sp. |
| title_full_unstemmed | Bacterial quorum sensing degrader from biofloc and freshwater prawn, Macrobrachium rosenbergii associated with green microalgae Chlorella sp. |
| title_short | Bacterial quorum sensing degrader from biofloc and freshwater prawn, Macrobrachium rosenbergii associated with green microalgae Chlorella sp. |
| title_sort | bacterial quorum sensing degrader from biofloc and freshwater prawn, macrobrachium rosenbergii associated with green microalgae chlorella sp. |
| url | http://psasir.upm.edu.my/id/eprint/77355/ http://psasir.upm.edu.my/id/eprint/77355/1/8TH%20IAC%20%E2%80%93%206TH%20ISFA%202018-36.pdf |