Extraction and determination of oryzanol in rice bran of mixed Herbarium UKMB; AZ 6807: MR 185, AZ 6808: MR 211, AZ6809: MR 29

The level of total lipid and oryzanol content, an important antioxidant compound in locally produced bran was investigated. Total lipid in rice bran was extracted using 3:2 chloroform:methanol mixture yielding 16.4% fat. Oryzanol content was determined without saponification using a reverse-phase HP...

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Main Authors: Azlan, Azrina, Ismail, Maznah, Abdul Hamid, Azizah
Format: Article
Language:English
Published: Universiti Putra Malaysia Press 2008
Online Access:http://psasir.upm.edu.my/id/eprint/773/
http://psasir.upm.edu.my/id/eprint/773/1/89-96.pdf
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author Azlan, Azrina
Ismail, Maznah
Abdul Hamid, Azizah
author_facet Azlan, Azrina
Ismail, Maznah
Abdul Hamid, Azizah
author_sort Azlan, Azrina
building UPM Institutional Repository
collection Online Access
description The level of total lipid and oryzanol content, an important antioxidant compound in locally produced bran was investigated. Total lipid in rice bran was extracted using 3:2 chloroform:methanol mixture yielding 16.4% fat. Oryzanol content was determined without saponification using a reverse-phase HPLC. Four fractions of oryzanol were successfully separated and quantitated. The 4 isomers were cycloartenyl ferulate, 24-methylene cycloartanyl ferulate, campestryl ferulate and mixtures of β–sitosteryl ferulate and cycloartanyl ferulate. The oryzanol content of local mixed varieties ranged from 23.7–43.0 mg g-1. The oryzanol concentration may depend on factors such as plant varieties, processing methods employed, extracting solvent used and ratio of extracting solvent to bran as well as extracting solvent temperatures. This study showed the potential of oryzanol extract from rice bran as a source of antioxidant.
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spelling upm-7732016-11-15T07:14:12Z http://psasir.upm.edu.my/id/eprint/773/ Extraction and determination of oryzanol in rice bran of mixed Herbarium UKMB; AZ 6807: MR 185, AZ 6808: MR 211, AZ6809: MR 29 Azlan, Azrina Ismail, Maznah Abdul Hamid, Azizah The level of total lipid and oryzanol content, an important antioxidant compound in locally produced bran was investigated. Total lipid in rice bran was extracted using 3:2 chloroform:methanol mixture yielding 16.4% fat. Oryzanol content was determined without saponification using a reverse-phase HPLC. Four fractions of oryzanol were successfully separated and quantitated. The 4 isomers were cycloartenyl ferulate, 24-methylene cycloartanyl ferulate, campestryl ferulate and mixtures of β–sitosteryl ferulate and cycloartanyl ferulate. The oryzanol content of local mixed varieties ranged from 23.7–43.0 mg g-1. The oryzanol concentration may depend on factors such as plant varieties, processing methods employed, extracting solvent used and ratio of extracting solvent to bran as well as extracting solvent temperatures. This study showed the potential of oryzanol extract from rice bran as a source of antioxidant. Universiti Putra Malaysia Press 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/773/1/89-96.pdf Azlan, Azrina and Ismail, Maznah and Abdul Hamid, Azizah (2008) Extraction and determination of oryzanol in rice bran of mixed Herbarium UKMB; AZ 6807: MR 185, AZ 6808: MR 211, AZ6809: MR 29. ASEAN Food Journal, 15 (1). pp. 89-96. ISSN 0127-7324; ESSN: 1505-5337 http://www.ifrj.upm.edu.my/afjv15%281%292008/89-96.pdf
spellingShingle Azlan, Azrina
Ismail, Maznah
Abdul Hamid, Azizah
Extraction and determination of oryzanol in rice bran of mixed Herbarium UKMB; AZ 6807: MR 185, AZ 6808: MR 211, AZ6809: MR 29
title Extraction and determination of oryzanol in rice bran of mixed Herbarium UKMB; AZ 6807: MR 185, AZ 6808: MR 211, AZ6809: MR 29
title_full Extraction and determination of oryzanol in rice bran of mixed Herbarium UKMB; AZ 6807: MR 185, AZ 6808: MR 211, AZ6809: MR 29
title_fullStr Extraction and determination of oryzanol in rice bran of mixed Herbarium UKMB; AZ 6807: MR 185, AZ 6808: MR 211, AZ6809: MR 29
title_full_unstemmed Extraction and determination of oryzanol in rice bran of mixed Herbarium UKMB; AZ 6807: MR 185, AZ 6808: MR 211, AZ6809: MR 29
title_short Extraction and determination of oryzanol in rice bran of mixed Herbarium UKMB; AZ 6807: MR 185, AZ 6808: MR 211, AZ6809: MR 29
title_sort extraction and determination of oryzanol in rice bran of mixed herbarium ukmb; az 6807: mr 185, az 6808: mr 211, az6809: mr 29
url http://psasir.upm.edu.my/id/eprint/773/
http://psasir.upm.edu.my/id/eprint/773/
http://psasir.upm.edu.my/id/eprint/773/1/89-96.pdf