The profile of enzymes relevant to solvent production during direct fermentation of sago starch by Clostridium saccharobutylicum P262 utilizing different pH control strategies
The profile of enzymes relevant to solvent production during direct fermentation of sago starch by Clostridium saccharobutylicum P262 in a 2 L stirred tank fermenter was determined utilizing different pH control strategies. During fermentation without pH control (initial pH of 6), the specific activ...
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| Format: | Article |
| Language: | English English |
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Springer
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/7698/ http://psasir.upm.edu.my/id/eprint/7698/1/The%20profile%20of%20enzymes%20relevant%20to%20solvent%20production%20during%20direct%20fermentation%20of%20sago%20starch%20by%20Clostridium%20saccharobutylicum%20P262%20utilizing%20different%20pH%20control%20strategies.pdf |
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| author | Md Salleh, Madihah Liew, Shiau Tsuey Ariff, Arbakariya |
| author_facet | Md Salleh, Madihah Liew, Shiau Tsuey Ariff, Arbakariya |
| author_sort | Md Salleh, Madihah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The profile of enzymes relevant to solvent production during direct fermentation of sago starch by Clostridium saccharobutylicum P262 in a 2 L stirred tank fermenter was determined utilizing different pH control strategies. During fermentation without pH control (initial pH of 6), the specific activity of crotonase, thiolase, and β-hydroxybutyryl-CoA dehydrogenase increased proportionally with solvent production. The highest crotonase (3,450.7 kat) and phosphotransbutyrylase activity (1,475.6 kat) was observed in fermentation where pH was maintained at 5 during the acidogenic phase and corresponded to a fairly high acid accumulation but low solvent production. During fermentation with a controlled pH of 5.25 during the sol-ventogenic phase, the highest thiolase specific activity (255.7 kat) was obtained and corresponded to the highest production of acetone. On the other hand, the highest specific activities of crotonase, β-hydroxybutyryl-CoA dehydrogenase, and phosphotransbutyrylase were observed at pH 5.5 and corresponded to the highest production of ethanol and butanol. Butyryl-CoA dehydrogenase had no significance role in solvent fermentation. These results suggested that pH control strategies were important for improvement of solvent production during direct fermentation of sago starch by C. saccharobutylicum. |
| first_indexed | 2025-11-15T07:31:00Z |
| format | Article |
| id | upm-7698 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:31:00Z |
| publishDate | 2008 |
| publisher | Springer |
| recordtype | eprints |
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| spelling | upm-76982015-10-26T01:39:36Z http://psasir.upm.edu.my/id/eprint/7698/ The profile of enzymes relevant to solvent production during direct fermentation of sago starch by Clostridium saccharobutylicum P262 utilizing different pH control strategies Md Salleh, Madihah Liew, Shiau Tsuey Ariff, Arbakariya The profile of enzymes relevant to solvent production during direct fermentation of sago starch by Clostridium saccharobutylicum P262 in a 2 L stirred tank fermenter was determined utilizing different pH control strategies. During fermentation without pH control (initial pH of 6), the specific activity of crotonase, thiolase, and β-hydroxybutyryl-CoA dehydrogenase increased proportionally with solvent production. The highest crotonase (3,450.7 kat) and phosphotransbutyrylase activity (1,475.6 kat) was observed in fermentation where pH was maintained at 5 during the acidogenic phase and corresponded to a fairly high acid accumulation but low solvent production. During fermentation with a controlled pH of 5.25 during the sol-ventogenic phase, the highest thiolase specific activity (255.7 kat) was obtained and corresponded to the highest production of acetone. On the other hand, the highest specific activities of crotonase, β-hydroxybutyryl-CoA dehydrogenase, and phosphotransbutyrylase were observed at pH 5.5 and corresponded to the highest production of ethanol and butanol. Butyryl-CoA dehydrogenase had no significance role in solvent fermentation. These results suggested that pH control strategies were important for improvement of solvent production during direct fermentation of sago starch by C. saccharobutylicum. Springer 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7698/1/The%20profile%20of%20enzymes%20relevant%20to%20solvent%20production%20during%20direct%20fermentation%20of%20sago%20starch%20by%20Clostridium%20saccharobutylicum%20P262%20utilizing%20different%20pH%20control%20strategies.pdf Md Salleh, Madihah and Liew, Shiau Tsuey and Ariff, Arbakariya (2008) The profile of enzymes relevant to solvent production during direct fermentation of sago starch by Clostridium saccharobutylicum P262 utilizing different pH control strategies. Biotechnology and Bioprocess Engineering, 13 (1). pp. 33-39. ISSN 1226-8372 http://dx.doi.org/10.1007/s12257-007-0153-2 10.1007/s12257-007-0153-2 English |
| spellingShingle | Md Salleh, Madihah Liew, Shiau Tsuey Ariff, Arbakariya The profile of enzymes relevant to solvent production during direct fermentation of sago starch by Clostridium saccharobutylicum P262 utilizing different pH control strategies |
| title | The profile of enzymes relevant to solvent production during direct fermentation of sago starch by Clostridium saccharobutylicum P262 utilizing different pH control strategies |
| title_full | The profile of enzymes relevant to solvent production during direct fermentation of sago starch by Clostridium saccharobutylicum P262 utilizing different pH control strategies |
| title_fullStr | The profile of enzymes relevant to solvent production during direct fermentation of sago starch by Clostridium saccharobutylicum P262 utilizing different pH control strategies |
| title_full_unstemmed | The profile of enzymes relevant to solvent production during direct fermentation of sago starch by Clostridium saccharobutylicum P262 utilizing different pH control strategies |
| title_short | The profile of enzymes relevant to solvent production during direct fermentation of sago starch by Clostridium saccharobutylicum P262 utilizing different pH control strategies |
| title_sort | profile of enzymes relevant to solvent production during direct fermentation of sago starch by clostridium saccharobutylicum p262 utilizing different ph control strategies |
| url | http://psasir.upm.edu.my/id/eprint/7698/ http://psasir.upm.edu.my/id/eprint/7698/ http://psasir.upm.edu.my/id/eprint/7698/ http://psasir.upm.edu.my/id/eprint/7698/1/The%20profile%20of%20enzymes%20relevant%20to%20solvent%20production%20during%20direct%20fermentation%20of%20sago%20starch%20by%20Clostridium%20saccharobutylicum%20P262%20utilizing%20different%20pH%20control%20strategies.pdf |