Verification of H5 and IL-15 gene integrations in recombinant fowlpox viruses
Introduction of the chicken cytokine gene might trigger a stronger cellular mediated immune response in recombinant fowlpox viruses already carrying the avian influenza H5 gene. In order to ascertain that integrated H5 and IL-15 genes are stable in the previously constructed recombinant fowlpox viru...
| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/75454/ http://psasir.upm.edu.my/id/eprint/75454/1/ICMSM2015-1.pdf |
| _version_ | 1848857692511141888 |
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| author | Nor Majid, Nadzreeq Abdul Razak, Mariatulqabtiah Roslan, Nurhidayah Omar, Abdul Rahman |
| author_facet | Nor Majid, Nadzreeq Abdul Razak, Mariatulqabtiah Roslan, Nurhidayah Omar, Abdul Rahman |
| author_sort | Nor Majid, Nadzreeq |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Introduction of the chicken cytokine gene might trigger a stronger cellular mediated immune response in recombinant fowlpox viruses already carrying the avian influenza H5 gene. In order to ascertain that integrated H5 and IL-15 genes are stable in the previously constructed recombinant fowlpox viruses, PCR analyses were done after a few viral passages. Wild type fowlpox virus, recombinant fowlpox viruses co-expressing avian influenza H5 (rFWPV/H5) and recombinant fowlpox viruses co-expressing avian influenza H5 and chicken IL-15 cytokine (rFWPV/H5) were propagated in CEF cell culture. DNA genomic extractions were done prior to PCR analysis. Primer set targeting H5 and IL-15 confirmed the deduced sizes of both genes. Flanking primers further verified the stable integration of H5 and IL-15 genes in both recombinant viruses. This study confirms that H5 and IL-15 genes were stably present rFWPV/H5 and rFWPV/H5/IL-15. The current results allow future in vitro and in vivo characterisation of the recombinant viruses in triggering humoral and cellular immune responses. |
| first_indexed | 2025-11-15T12:01:35Z |
| format | Conference or Workshop Item |
| id | upm-75454 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:01:35Z |
| publishDate | 2015 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-754542019-10-07T04:21:27Z http://psasir.upm.edu.my/id/eprint/75454/ Verification of H5 and IL-15 gene integrations in recombinant fowlpox viruses Nor Majid, Nadzreeq Abdul Razak, Mariatulqabtiah Roslan, Nurhidayah Omar, Abdul Rahman Introduction of the chicken cytokine gene might trigger a stronger cellular mediated immune response in recombinant fowlpox viruses already carrying the avian influenza H5 gene. In order to ascertain that integrated H5 and IL-15 genes are stable in the previously constructed recombinant fowlpox viruses, PCR analyses were done after a few viral passages. Wild type fowlpox virus, recombinant fowlpox viruses co-expressing avian influenza H5 (rFWPV/H5) and recombinant fowlpox viruses co-expressing avian influenza H5 and chicken IL-15 cytokine (rFWPV/H5) were propagated in CEF cell culture. DNA genomic extractions were done prior to PCR analysis. Primer set targeting H5 and IL-15 confirmed the deduced sizes of both genes. Flanking primers further verified the stable integration of H5 and IL-15 genes in both recombinant viruses. This study confirms that H5 and IL-15 genes were stably present rFWPV/H5 and rFWPV/H5/IL-15. The current results allow future in vitro and in vivo characterisation of the recombinant viruses in triggering humoral and cellular immune responses. 2015 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/75454/1/ICMSM2015-1.pdf Nor Majid, Nadzreeq and Abdul Razak, Mariatulqabtiah and Roslan, Nurhidayah and Omar, Abdul Rahman (2015) Verification of H5 and IL-15 gene integrations in recombinant fowlpox viruses. In: International Congress of the Malaysian Society for Microbiology (ICMSM 2015), 7-10 Dec. 2015, Bayview Beach Resort, Penang, Malaysia. (pp. 50-54). |
| spellingShingle | Nor Majid, Nadzreeq Abdul Razak, Mariatulqabtiah Roslan, Nurhidayah Omar, Abdul Rahman Verification of H5 and IL-15 gene integrations in recombinant fowlpox viruses |
| title | Verification of H5 and IL-15 gene integrations in recombinant fowlpox viruses |
| title_full | Verification of H5 and IL-15 gene integrations in recombinant fowlpox viruses |
| title_fullStr | Verification of H5 and IL-15 gene integrations in recombinant fowlpox viruses |
| title_full_unstemmed | Verification of H5 and IL-15 gene integrations in recombinant fowlpox viruses |
| title_short | Verification of H5 and IL-15 gene integrations in recombinant fowlpox viruses |
| title_sort | verification of h5 and il-15 gene integrations in recombinant fowlpox viruses |
| url | http://psasir.upm.edu.my/id/eprint/75454/ http://psasir.upm.edu.my/id/eprint/75454/1/ICMSM2015-1.pdf |