Antioxidant properties of Morinda citrifolia L. and their effects on haemostasis parameters
This study aims to determine the antioxidant properties of Morinda citrifolia (M. citrifolia) fruit crude extracts and its effects on the blood haemostasis parameters. Extracts used in this study were M. citrifolia fruit crude aqueous and ethanolic extracts (MFCAE and MFCEE). 2,2-diphenyl-1-pi...
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| Format: | Thesis |
| Language: | English |
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2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/75402/ http://psasir.upm.edu.my/id/eprint/75402/1/FPSK%28M%29%202014%2022%20IR.pdf |
| _version_ | 1848857678033453056 |
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| author | Abdul Karim, Mohd Arif |
| author_facet | Abdul Karim, Mohd Arif |
| author_sort | Abdul Karim, Mohd Arif |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study aims to determine the antioxidant properties of Morinda citrifolia (M.
citrifolia) fruit crude extracts and its effects on the blood haemostasis parameters.
Extracts used in this study were M. citrifolia fruit crude aqueous and ethanolic extracts
(MFCAE and MFCEE). 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging
and β-carotene bleaching assay were conducted to determine antioxidant activity.
Ascorbic acid (ASA) and butylated hydroxytoluene (BHT) were used as standard
antioxidants. Total phenolic content was determined spectrometrically according to the
Folin-Ciocalteu’s method and expressed as Gallic acid equivalent (GAE). The effects on
blood haemostasis parameters were determined by using in vitro and in vivo models. In
vitro model was done by determination the effect on haemostasis tests which include
prothrombin time (PT), activated partial thrombosplastin time (APTT), and platelet
aggregation tests where the human blood samples were treated with different
concentrations (10, 20, 30, 40, 50mg/mL) of MFCAE and MFCEE. Blood collected
from 58 respondents were prepared to obtain platelet rich plasma (PRP) and platelet
poor plasma (PPP) for coagulation (PT, APTT), and platelet aggregation test
respectively. In in vivo model, 48 Sprague Dawley rats were treated with different
dosage (7.5, 75, 750mg/kg) of MFCAE. Their blood samples were subjected for haemostasis parameter tests which include full blood count, PT, APTT, platelet
aggregation, and bleeding time. The highest percentage of inhibition by MFCAE against
DPPH radicals was 27.21 ± 1.24%, 61.78 % lower when compared to ASA. β-carotene
bleaching assay showed the percentage of antioxidant activity of MFCAE was 78.96%,
13.6% lower than BHT. Total phenolic content of MFCAE was 281.83 ± 14.78mg
GAE/100g. In vitro model showed that MFCAE and MFCEE were significantly
prolonged the PT and APTT in dose dependant manner. The highest measureable value
was at the concentration of 40mg/mL. The highest PT and APTT were 55.97 ± 14.54
and 114.74 ± 11.53 seconds for MFCAE; and 58.27 ± 15.69 and 118.03 ± 10.18 seconds
respectively for MFCEE. However this anticoagulant property for the in vivo model was
not significantly observed as in vitro model. MFCAE exhibits some antiplatelet activity
in both in vitro and in vivo model. This study showed MFCAE contains some
antiplatelet activity with dose dependant manner when collagen was used as an agonist.
Briefly, the findings suggest that M. citrifolia extracts may become a potential plant
based anticoagulant and antiplatelet which should be effective and safe for patients with
cardiovascular disorders. The presence of total phenolic in the extract may be partly
responsible for the observed effects. |
| first_indexed | 2025-11-15T12:01:22Z |
| format | Thesis |
| id | upm-75402 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:01:22Z |
| publishDate | 2014 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-754022025-06-11T01:09:01Z http://psasir.upm.edu.my/id/eprint/75402/ Antioxidant properties of Morinda citrifolia L. and their effects on haemostasis parameters Abdul Karim, Mohd Arif This study aims to determine the antioxidant properties of Morinda citrifolia (M. citrifolia) fruit crude extracts and its effects on the blood haemostasis parameters. Extracts used in this study were M. citrifolia fruit crude aqueous and ethanolic extracts (MFCAE and MFCEE). 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and β-carotene bleaching assay were conducted to determine antioxidant activity. Ascorbic acid (ASA) and butylated hydroxytoluene (BHT) were used as standard antioxidants. Total phenolic content was determined spectrometrically according to the Folin-Ciocalteu’s method and expressed as Gallic acid equivalent (GAE). The effects on blood haemostasis parameters were determined by using in vitro and in vivo models. In vitro model was done by determination the effect on haemostasis tests which include prothrombin time (PT), activated partial thrombosplastin time (APTT), and platelet aggregation tests where the human blood samples were treated with different concentrations (10, 20, 30, 40, 50mg/mL) of MFCAE and MFCEE. Blood collected from 58 respondents were prepared to obtain platelet rich plasma (PRP) and platelet poor plasma (PPP) for coagulation (PT, APTT), and platelet aggregation test respectively. In in vivo model, 48 Sprague Dawley rats were treated with different dosage (7.5, 75, 750mg/kg) of MFCAE. Their blood samples were subjected for haemostasis parameter tests which include full blood count, PT, APTT, platelet aggregation, and bleeding time. The highest percentage of inhibition by MFCAE against DPPH radicals was 27.21 ± 1.24%, 61.78 % lower when compared to ASA. β-carotene bleaching assay showed the percentage of antioxidant activity of MFCAE was 78.96%, 13.6% lower than BHT. Total phenolic content of MFCAE was 281.83 ± 14.78mg GAE/100g. In vitro model showed that MFCAE and MFCEE were significantly prolonged the PT and APTT in dose dependant manner. The highest measureable value was at the concentration of 40mg/mL. The highest PT and APTT were 55.97 ± 14.54 and 114.74 ± 11.53 seconds for MFCAE; and 58.27 ± 15.69 and 118.03 ± 10.18 seconds respectively for MFCEE. However this anticoagulant property for the in vivo model was not significantly observed as in vitro model. MFCAE exhibits some antiplatelet activity in both in vitro and in vivo model. This study showed MFCAE contains some antiplatelet activity with dose dependant manner when collagen was used as an agonist. Briefly, the findings suggest that M. citrifolia extracts may become a potential plant based anticoagulant and antiplatelet which should be effective and safe for patients with cardiovascular disorders. The presence of total phenolic in the extract may be partly responsible for the observed effects. 2014-06 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/75402/1/FPSK%28M%29%202014%2022%20IR.pdf Abdul Karim, Mohd Arif (2014) Antioxidant properties of Morinda citrifolia L. and their effects on haemostasis parameters. Masters thesis, Universiti Putra Malaysia. http://ethesis.upm.edu.my/id/eprint/12732/ Antioxidants - chemistry Antioxidants - chemical synthesis Morinda - chemistry |
| spellingShingle | Antioxidants - chemistry Antioxidants - chemical synthesis Morinda - chemistry Abdul Karim, Mohd Arif Antioxidant properties of Morinda citrifolia L. and their effects on haemostasis parameters |
| title | Antioxidant properties of Morinda citrifolia L. and their effects on haemostasis parameters |
| title_full | Antioxidant properties of Morinda citrifolia L. and their effects on haemostasis parameters |
| title_fullStr | Antioxidant properties of Morinda citrifolia L. and their effects on haemostasis parameters |
| title_full_unstemmed | Antioxidant properties of Morinda citrifolia L. and their effects on haemostasis parameters |
| title_short | Antioxidant properties of Morinda citrifolia L. and their effects on haemostasis parameters |
| title_sort | antioxidant properties of morinda citrifolia l. and their effects on haemostasis parameters |
| topic | Antioxidants - chemistry Antioxidants - chemical synthesis Morinda - chemistry |
| url | http://psasir.upm.edu.my/id/eprint/75402/ http://psasir.upm.edu.my/id/eprint/75402/ http://psasir.upm.edu.my/id/eprint/75402/1/FPSK%28M%29%202014%2022%20IR.pdf |