High-temperature crystallization of thermostable T1 lipase

QR Code

High-temperature crystallization of thermostable T1 lipase

The gene encoding thermostable T1 lipase secreted by Geobacillus sp. strain T1 has been overexpressed in a prokaryotic system. Preliminary crystallization was conducted with crystal screen and crystal screen II through a sitting drop vapor diffusion method with 0.5 mg/mL purified T1 lipase at 16 °C....

Full description

Bibliographic Details
Main Authors:	Leow, Thean Chor, Raja Abdul Rahman, Raja Noor Zaliha, Salleh, Abu Bakar, Basri, Mahiran
Format:	Article
Language:	English
Published:	American Chemical Society 2007
Online Access:	http://psasir.upm.edu.my/id/eprint/7524/ http://psasir.upm.edu.my/id/eprint/7524/1/High-Temperature%20Crystallization%20of%20Thermostable%20T1%20Lipase.pdf

Similar Items

The effects of microgravity on thermostable T1 lipase protein crystal
by: Raja Abdul Rahman, Raja Noor Zaliha, et al.
Published: (2010)

High level expression of thermostable lipase from Geobacillus sp. strain T1
by: Leow, Thean Chor, et al.
Published: (2004)

Method for producing a recombinant thermostable geobacillus T1 lipase
by: Raja Abdul Rahman, Raja Noor Zaliha, et al.
Published: (2008)

Thermostable lipases
by: Leow, Adam Thean Chor, et al.
Published: (2006)

Secretory expression of thermostable T1 lipase through bacteriocin release protein
by: Raja Abdul Rahman, Raja Noor Zaliha, et al.
Published: (2005)

Crystallization and structure elucidation of thermostable lipases
by: Mohd Shariff, Fairolniza, et al.
Published: (2013)

Secretory expression and characterization of a highly Ca2+-activated thermostable L2 lipase
by: Sabri, Suriana, et al.
Published: (2009)

Pichia pastoris as a host to overexpress the thermostable T1 lipase from Geobacillus zalihae
by: Oslan, Siti Nurbaya, et al.
Published: (2014)

A comparative analysis of microgravity and earth grown thermostable T1 lipase crystals using HDPCG apparatus
by: Raja Abdul Rahman, Raja Noor Zaliha, et al.
Published: (2015)

Crystallographic analysis of ground and space thermostable T1 lipase crystal obtained via counter diffusion method approach
by: Mohamad Aris, Sayangku Nor Ariati, et al.
Published: (2014)

Heterologous expression of industrially important thermostable lipase
by: Leow, Adam Thean Chor, et al.
Published: (2013)

Crystallization and preliminary X-ray crystallographic analysis of highly thermostable L2 lipase from the newly isolated Bacillus sp. L2
by: Mohd Shariff, Fairolniza, et al.
Published: (2010)

On the importance of the small domain in the thermostability of thermoalkalophilic lipases from L1 and T1: insights from molecular dynamics simulation
by: Karjiban, Roghayeh Abedi, et al.
Published: (2010)

Crystallization and preliminary X-ray crystallographic analysis of a thermostable organic solvent-tolerant lipase from Bacillus sp. strain 42
by: Khusaini, Mohd Saif, et al.
Published: (2011)

The effect of carbon sources on the expression level of thermostable L2 lipase in Pichia pastoris
by: Shahidan, Noor Hidayah, et al.
Published: (2011)

A thermoalkaliphilic lipase of Geobacillus sp. T1
by: Leow, Thean Chor, et al.
Published: (2007)

Lipase from Geobacillus Sp. Strain T1
by: Raja Abdul Rahman, Raja Noor Zaliha, et al.
Published: (2008)

Thermostable proteases
by: Ibrahim, Noor Azlina, et al.
Published: (2006)

Reductive alkylation causes the formation of a molten globule-like intermediate structure in Geobacillus zalihae strain T1 thermostable lipase
by: Cheong, Kok Whye, et al.
Published: (2011)

Crystallization of N-terminal Strep-tagged Fusion Lipase from Thermostable Bacillus sp. Strain 42.
by: Basri, Mahiran, et al.
Published: (2009)

Purification and biochemical characterization of extracellular thermostable lipase from Bacillus sp. strain L2
by: Nezhad, Nima Ghahremani, et al.
Published: (2024)

A newly isolated thermostable lipase from Bacillus sp.
by: Mohd Shariff, Fairolniza, et al.
Published: (2011)

High level expression and characterization of a novel thermostable, organic solvent tolerant, 1,3-regioselective lipase from Geobacillus sp. strain ARM
by: Ebrahimpour, Afshin, et al.
Published: (2011)

A host-vector system for the expression of a thermostable bacterial lipase in a locally isolated Meyerozyma guilliermondii SMB
by: Salleh, Abu Bakar, et al.
Published: (2020)

A new thermostable lipase by Aneurinibacillus thermoaerophilus strain HZ: nutritional studies
by: Raja Abdul Rahman, Raja Noor Zaliha, et al.
Published: (2009)

Production of thermostable L2 lipase from bacillus sp.
by: Raja Abdul Rahman, Raja Noor Zaliha, et al.
Published: (2007)

Novel microorganisms producing thermostable lipase from oil contaminated sample and methods thereof
by: Raja Abdul Rahman, Raja Noor Zaliha, et al.
Published: (2010)

Use of a small domain of the T1 lipase as a protein scaffold
by: Leow, Adam Thean Chor, et al.
Published: (2012)

Purified T1 lipase, a novel construct of T1 lipase and methods thereoF
by: Raja Abdul Rahman, Raja Noor Zaliha, et al.
Published: (2011)

Molecular dynamics study of the structure, flexibility and dynamics of thermostable L1 lipase at high temperatures.
by: Abedi Karjiban, Roghayeh, et al.
Published: (2009)

Deciphering the flexibility and dynamics of Geobacillus zalihae strain T1 lipase at high temperatures by molecular dynamics simulation
by: Abdul Rahman, Mohd Basyaruddin, et al.
Published: (2009)

A new thermostable and organic solvent-tolerant lipase from Aneurinibacillus thermoaerophilus strain HZ
by: Masomian, Malihe, et al.
Published: (2013)

Crystallization of enzyme and method for producing same
by: Raja Abdul Rahman, Raja Noor Zaliha, et al.
Published: (2006)

The effects of one amino acid substitutions at the C-terminal region of thermostable L2 lipase by computational and experimental approach
by: Ahmad Sani, Hartini, et al.
Published: (2018)

Effects of lid 1 mutagenesis on lid displacement, catalytic performances and thermostability of cold-active Pseudomonas AMS8 lipase in toluene
by: Yaacob, Norhayati, et al.
Published: (2019)

Novel cation-π interaction revealed by crystal structure of thermoalkalophilic lipase
by: Matsumura, Hiroyoshi, et al.
Published: (2008)

Molten Globule-Triggered Inactivation of a thermostable and solvent stable lipase in hydrophilic Solvents
by: Tengku Abdul Hamid, Tengku Haziyamin, et al.
Published: (2010)

Molten globule-triggered inactivation of a thermostable and solvent stable lipase in hydrophilic solvents
by: Tengku Abdul Hamid, Tengku Haziyamin, et al.
Published: (2010)

Crystal growth under microgravity condition
by: Raja Abdul Rahman, Raja Noor Zaliha, et al.
Published: (2008)

The effect of single N-terminal mutation to the properties of thermostable L2 lipase
by: Raja Abdul Rahman, Raja Noor Zaliha, et al.
Published: (2008)