Pathological changes and bacteriological assessments in the urinary tract of the pregnant goats experimentally infected with Brucella melitensis
Background: This study was conducted to investigate the pathological changes and distribution of B. melitensis in the urinary tract of pregnant goats following acute experimental infection. Six Jamnapari crossbred does in their third trimester of pregnancy were randomly assigned into two groups; Gr...
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| Format: | Article |
| Language: | English |
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BioMed Central
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/75211/ http://psasir.upm.edu.my/id/eprint/75211/1/Pathological%20changes%20and%20bacteriological.pdf |
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| author | Mazlan, Mazlina Bejo, Siti Khairani Hamzah, Hazilawati Tiagarahan, T. Shaqinah, N. N. Saad, Mohd. Zamri |
| author_facet | Mazlan, Mazlina Bejo, Siti Khairani Hamzah, Hazilawati Tiagarahan, T. Shaqinah, N. N. Saad, Mohd. Zamri |
| author_sort | Mazlan, Mazlina |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background: This study was conducted to investigate the pathological changes and distribution of B. melitensis in
the urinary tract of pregnant goats following acute experimental infection. Six Jamnapari crossbred does in their third trimester of pregnancy were randomly assigned into two groups; Group 1 was uninfected control and Group
2 was inoculated conjunctival with 0.1 mL of the inoculums containing 109cfu/mL of live B. melitensis. All does
were sacrificed 30 days post-inoculation before the kidney, ureter, urinary bladder, urethra and vaginal swab were
collected for isolation of B. melitensis. The same tissue samples were fixed in 10% neutral buffered formalin for
hematoxylin and eosin, and immunoperoxidase staining.
Results: None of the goats showed clinical signs or gross lesions. The most consistent histopathology finding was
the infiltration of mononuclear cells, chiefly the macrophages with few lymphocytes and occasionally neutrophils in all organs along the urinary tract of the infected goats of Group 2. Other histopathology findings included mildnecrosis of the epithelial cells of the renal tubules, congestion and occasional haemorrhages in the various tissues.Kidneys showed the most severe lesions. Immunoperoxidase staining revealed the presence of B. melitensis within the infiltrating macrophages and the epithelium of renal tubules, ureter, urethra and urinary bladder. Most extensive distribution was observed in the urinary bladder. Brucella melitensis was successfully isolated at low concentration (3.4×103cfu/g) in the various organs of the urinary tract and at high concentration (2.4 × 108cfu/mL) in the vaginal swabs of all infected goats. Although B. melitensis was successfully isolated from the various organs of the urinary tract, it was not isolated from the urine samples that were collected from the urinary bladder at necropsy.Conclusion: This study demonstrates the presence of low concentrations of B. melitensis in the organs of urinary tract of pregnant does, resulting in mild histopathology lesions. However, B. melitensis was not isolated from the urine that was collected from the urinary bladder. |
| first_indexed | 2025-11-15T12:00:36Z |
| format | Article |
| id | upm-75211 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:00:36Z |
| publishDate | 2018 |
| publisher | BioMed Central |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-752112019-10-14T05:25:40Z http://psasir.upm.edu.my/id/eprint/75211/ Pathological changes and bacteriological assessments in the urinary tract of the pregnant goats experimentally infected with Brucella melitensis Mazlan, Mazlina Bejo, Siti Khairani Hamzah, Hazilawati Tiagarahan, T. Shaqinah, N. N. Saad, Mohd. Zamri Background: This study was conducted to investigate the pathological changes and distribution of B. melitensis in the urinary tract of pregnant goats following acute experimental infection. Six Jamnapari crossbred does in their third trimester of pregnancy were randomly assigned into two groups; Group 1 was uninfected control and Group 2 was inoculated conjunctival with 0.1 mL of the inoculums containing 109cfu/mL of live B. melitensis. All does were sacrificed 30 days post-inoculation before the kidney, ureter, urinary bladder, urethra and vaginal swab were collected for isolation of B. melitensis. The same tissue samples were fixed in 10% neutral buffered formalin for hematoxylin and eosin, and immunoperoxidase staining. Results: None of the goats showed clinical signs or gross lesions. The most consistent histopathology finding was the infiltration of mononuclear cells, chiefly the macrophages with few lymphocytes and occasionally neutrophils in all organs along the urinary tract of the infected goats of Group 2. Other histopathology findings included mildnecrosis of the epithelial cells of the renal tubules, congestion and occasional haemorrhages in the various tissues.Kidneys showed the most severe lesions. Immunoperoxidase staining revealed the presence of B. melitensis within the infiltrating macrophages and the epithelium of renal tubules, ureter, urethra and urinary bladder. Most extensive distribution was observed in the urinary bladder. Brucella melitensis was successfully isolated at low concentration (3.4×103cfu/g) in the various organs of the urinary tract and at high concentration (2.4 × 108cfu/mL) in the vaginal swabs of all infected goats. Although B. melitensis was successfully isolated from the various organs of the urinary tract, it was not isolated from the urine samples that were collected from the urinary bladder at necropsy.Conclusion: This study demonstrates the presence of low concentrations of B. melitensis in the organs of urinary tract of pregnant does, resulting in mild histopathology lesions. However, B. melitensis was not isolated from the urine that was collected from the urinary bladder. BioMed Central 2018-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/75211/1/Pathological%20changes%20and%20bacteriological.pdf Mazlan, Mazlina and Bejo, Siti Khairani and Hamzah, Hazilawati and Tiagarahan, T. and Shaqinah, N. N. and Saad, Mohd. Zamri (2018) Pathological changes and bacteriological assessments in the urinary tract of the pregnant goats experimentally infected with Brucella melitensis. BMC Veterinary Research, 14 (1). pp. 1-7. ISSN 1746-6148 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6019509/ 10.1186/s12917-018-1533-x |
| spellingShingle | Mazlan, Mazlina Bejo, Siti Khairani Hamzah, Hazilawati Tiagarahan, T. Shaqinah, N. N. Saad, Mohd. Zamri Pathological changes and bacteriological assessments in the urinary tract of the pregnant goats experimentally infected with Brucella melitensis |
| title | Pathological changes and bacteriological assessments in the urinary tract of the pregnant goats experimentally infected with Brucella melitensis |
| title_full | Pathological changes and bacteriological assessments in the urinary tract of the pregnant goats experimentally infected with Brucella melitensis |
| title_fullStr | Pathological changes and bacteriological assessments in the urinary tract of the pregnant goats experimentally infected with Brucella melitensis |
| title_full_unstemmed | Pathological changes and bacteriological assessments in the urinary tract of the pregnant goats experimentally infected with Brucella melitensis |
| title_short | Pathological changes and bacteriological assessments in the urinary tract of the pregnant goats experimentally infected with Brucella melitensis |
| title_sort | pathological changes and bacteriological assessments in the urinary tract of the pregnant goats experimentally infected with brucella melitensis |
| url | http://psasir.upm.edu.my/id/eprint/75211/ http://psasir.upm.edu.my/id/eprint/75211/ http://psasir.upm.edu.my/id/eprint/75211/ http://psasir.upm.edu.my/id/eprint/75211/1/Pathological%20changes%20and%20bacteriological.pdf |