Overexpression of acetyl-CoA carboxylase in Aspergillus terreus to increase lovastatin production
The present work describes the application of homologous recombination techniques in a wild-type Aspergillus terreus (ATCC 20542) strain to increase the flow of precursors towards the lovastatin biosynthesis pathway. A new strain was generated to overexpress acetyl-CoA carboxylase (ACCase) by replac...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/75182/ http://psasir.upm.edu.my/id/eprint/75182/1/Overexpression.pdf |
| _version_ | 1848857624105189376 |
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| author | Hasan, Hanan Abd Rahim, Muhammad Hafiz Campbell, Leona Carter, Dee Abbas, Ali Montoya, Alejandro |
| author_facet | Hasan, Hanan Abd Rahim, Muhammad Hafiz Campbell, Leona Carter, Dee Abbas, Ali Montoya, Alejandro |
| author_sort | Hasan, Hanan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The present work describes the application of homologous recombination techniques in a wild-type Aspergillus terreus (ATCC 20542) strain to increase the flow of precursors towards the lovastatin biosynthesis pathway. A new strain was generated to overexpress acetyl-CoA carboxylase (ACCase) by replacing the native ACCase promoter with a strong constitutive PadhA promoter from Aspergillus nidulans. Glycerol and a mixture of lactose and glycerol were used independently as the carbon feedstock to determine the degree of response by the A. terreus strains towards the production of acetyl-CoA, and malonyl-CoA. The new strain increased the levels of malonyl-CoA and acetyl-CoA by 240% and 14%, respectively, compared to the wild-type strain. As a result, lovastatin production was increased by 40% and (+)-geodin was decreased by 31% using the new strain. This study shows for the first time that the metabolism of Aspergillus terreus can be manipulated to attain higher levels of precursors and valuable secondary metabolites. |
| first_indexed | 2025-11-15T12:00:30Z |
| format | Article |
| id | upm-75182 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:00:30Z |
| publishDate | 2018 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-751822021-09-08T09:22:46Z http://psasir.upm.edu.my/id/eprint/75182/ Overexpression of acetyl-CoA carboxylase in Aspergillus terreus to increase lovastatin production Hasan, Hanan Abd Rahim, Muhammad Hafiz Campbell, Leona Carter, Dee Abbas, Ali Montoya, Alejandro The present work describes the application of homologous recombination techniques in a wild-type Aspergillus terreus (ATCC 20542) strain to increase the flow of precursors towards the lovastatin biosynthesis pathway. A new strain was generated to overexpress acetyl-CoA carboxylase (ACCase) by replacing the native ACCase promoter with a strong constitutive PadhA promoter from Aspergillus nidulans. Glycerol and a mixture of lactose and glycerol were used independently as the carbon feedstock to determine the degree of response by the A. terreus strains towards the production of acetyl-CoA, and malonyl-CoA. The new strain increased the levels of malonyl-CoA and acetyl-CoA by 240% and 14%, respectively, compared to the wild-type strain. As a result, lovastatin production was increased by 40% and (+)-geodin was decreased by 31% using the new strain. This study shows for the first time that the metabolism of Aspergillus terreus can be manipulated to attain higher levels of precursors and valuable secondary metabolites. Elsevier 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/75182/1/Overexpression.pdf Hasan, Hanan and Abd Rahim, Muhammad Hafiz and Campbell, Leona and Carter, Dee and Abbas, Ali and Montoya, Alejandro (2018) Overexpression of acetyl-CoA carboxylase in Aspergillus terreus to increase lovastatin production. New Biotechnology, 44. 64 - 71. ISSN 1871-6784; ESSN: 1876-4347 https://www.sciencedirect.com/science/article/abs/pii/S1871678417306763?via%3Dihub 10.1016/j.nbt.2018.04.008 |
| spellingShingle | Hasan, Hanan Abd Rahim, Muhammad Hafiz Campbell, Leona Carter, Dee Abbas, Ali Montoya, Alejandro Overexpression of acetyl-CoA carboxylase in Aspergillus terreus to increase lovastatin production |
| title | Overexpression of acetyl-CoA carboxylase in Aspergillus terreus to increase lovastatin production |
| title_full | Overexpression of acetyl-CoA carboxylase in Aspergillus terreus to increase lovastatin production |
| title_fullStr | Overexpression of acetyl-CoA carboxylase in Aspergillus terreus to increase lovastatin production |
| title_full_unstemmed | Overexpression of acetyl-CoA carboxylase in Aspergillus terreus to increase lovastatin production |
| title_short | Overexpression of acetyl-CoA carboxylase in Aspergillus terreus to increase lovastatin production |
| title_sort | overexpression of acetyl-coa carboxylase in aspergillus terreus to increase lovastatin production |
| url | http://psasir.upm.edu.my/id/eprint/75182/ http://psasir.upm.edu.my/id/eprint/75182/ http://psasir.upm.edu.my/id/eprint/75182/ http://psasir.upm.edu.my/id/eprint/75182/1/Overexpression.pdf |