Optimization of sub-critical water extraction (SWE) of lipid and eicosapentaenoic acid (EPA) from Nannochloropsis gaditana
Microalgae are a promising source of omega-3. The purpose of this study was to extract lipid with a relatively high content of eicosapentaenoic acid (EPA) from Nannochloropsis gaditana using subcritical water extraction (SWE). The effects of different temperatures (156.1-273.9°C), extraction times (...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/75167/ http://psasir.upm.edu.my/id/eprint/75167/1/Optimization%20of%20sub-critical%20water%20extraction%20%28SWE%29%20of%20lipid%20and%20eicosapentaenoic%20acid%20%28EPA%29%20from%20Nannochloropsis%20gaditana.pdf |
| Summary: | Microalgae are a promising source of omega-3. The purpose of this study was to extract lipid with a relatively high content of eicosapentaenoic acid (EPA) from Nannochloropsis gaditana using subcritical water extraction (SWE). The effects of different temperatures (156.1-273.9°C), extraction times (6.6-23.4 minutes), and biomass loadings (33-117 g algae/L) on the extraction yield were studied. From the optimization study using central composite design (CCD), quadratic models generated for lipid yield and EPA composition were considered to be significant models (p < 0.05). The predictive equations were also formed for lipid yield and EPA composition. The predicted optimum lipid yield and EPA composition at 236.54°C, 13.95 minutes, and 60.50 g algae/L were 18.278 wt% of total biomass and 14.036 wt% of total fatty acid methyl ester (FAME), respectively. |
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