Optimization of recovery of esterase from Serratia marcescens using combination of the solvent impregnated resin and aqueous two-phase extraction techniques
The performance of tunable aqueous polymer phase impregnated resins (TAPPIR) which is the combination of the solvent impregnated resin principle and an aqueous two-phase system for the separation of esterase from Serratia marcescens was evaluated in this study. Different molecular weight of polyethy...
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| Format: | Article |
| Language: | English |
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Taylor & Francis
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/75166/ http://psasir.upm.edu.my/id/eprint/75166/1/Optimization%2033.pdf |
| _version_ | 1848857620753940480 |
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| author | Abdul Aziz, Nur Fazrin Husna Abbasiliasi, Sahar Ariff, Arbakariya Hui, Suan Ng John Chi, Wei Lan Ahmad, Rosma Joo, Shun Tan |
| author_facet | Abdul Aziz, Nur Fazrin Husna Abbasiliasi, Sahar Ariff, Arbakariya Hui, Suan Ng John Chi, Wei Lan Ahmad, Rosma Joo, Shun Tan |
| author_sort | Abdul Aziz, Nur Fazrin Husna |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The performance of tunable aqueous polymer phase impregnated resins (TAPPIR) which is the combination of the solvent impregnated resin principle and an aqueous two-phase system for the separation of esterase from Serratia marcescens was evaluated in this study. Different molecular weight of polyethylene glycol (PEG) (2000, 4000 and 6000) at concentration ranging from 5% to 20% (w/w) and potassium citrate were used to construct the aqueous phase in TAPPIR technology. Optimum composition of PEG and salt for esterase partitioning was determined using response surface methodology. The optimum condition for the purification of esterase was impregnation of 25% (w/w) of PEG 2000 into 4 mm porous glass beads and extraction of esterase using 15% (w/w) potassium citrate at pH 8 containing 12% (w/w) crude loading with the addition of 4% (w/w) NaCl. Esterase from S. marcescens was successfully purified by the TAPPIR technology up to 5.32 of purification factor with a yield of 75.98%. |
| first_indexed | 2025-11-15T12:00:27Z |
| format | Article |
| id | upm-75166 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:00:27Z |
| publishDate | 2018 |
| publisher | Taylor & Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-751662019-11-27T03:49:43Z http://psasir.upm.edu.my/id/eprint/75166/ Optimization of recovery of esterase from Serratia marcescens using combination of the solvent impregnated resin and aqueous two-phase extraction techniques Abdul Aziz, Nur Fazrin Husna Abbasiliasi, Sahar Ariff, Arbakariya Hui, Suan Ng John Chi, Wei Lan Ahmad, Rosma Joo, Shun Tan The performance of tunable aqueous polymer phase impregnated resins (TAPPIR) which is the combination of the solvent impregnated resin principle and an aqueous two-phase system for the separation of esterase from Serratia marcescens was evaluated in this study. Different molecular weight of polyethylene glycol (PEG) (2000, 4000 and 6000) at concentration ranging from 5% to 20% (w/w) and potassium citrate were used to construct the aqueous phase in TAPPIR technology. Optimum composition of PEG and salt for esterase partitioning was determined using response surface methodology. The optimum condition for the purification of esterase was impregnation of 25% (w/w) of PEG 2000 into 4 mm porous glass beads and extraction of esterase using 15% (w/w) potassium citrate at pH 8 containing 12% (w/w) crude loading with the addition of 4% (w/w) NaCl. Esterase from S. marcescens was successfully purified by the TAPPIR technology up to 5.32 of purification factor with a yield of 75.98%. Taylor & Francis 2018-07-26 Article NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/75166/1/Optimization%2033.pdf Abdul Aziz, Nur Fazrin Husna and Abbasiliasi, Sahar and Ariff, Arbakariya and Hui, Suan Ng and John Chi, Wei Lan and Ahmad, Rosma and Joo, Shun Tan (2018) Optimization of recovery of esterase from Serratia marcescens using combination of the solvent impregnated resin and aqueous two-phase extraction techniques. Separation Science and Technology, 53 (18). 2952 - 2960. ISSN 0149-6395; ESSN:1520-5754 https://www.tandfonline.com/doi/abs/10.1080/01496395.2018.1497653 10.1080/01496395.2018.1497653 |
| spellingShingle | Abdul Aziz, Nur Fazrin Husna Abbasiliasi, Sahar Ariff, Arbakariya Hui, Suan Ng John Chi, Wei Lan Ahmad, Rosma Joo, Shun Tan Optimization of recovery of esterase from Serratia marcescens using combination of the solvent impregnated resin and aqueous two-phase extraction techniques |
| title | Optimization of recovery of esterase from Serratia marcescens using combination of the solvent impregnated resin and aqueous two-phase extraction techniques |
| title_full | Optimization of recovery of esterase from Serratia marcescens using combination of the solvent impregnated resin and aqueous two-phase extraction techniques |
| title_fullStr | Optimization of recovery of esterase from Serratia marcescens using combination of the solvent impregnated resin and aqueous two-phase extraction techniques |
| title_full_unstemmed | Optimization of recovery of esterase from Serratia marcescens using combination of the solvent impregnated resin and aqueous two-phase extraction techniques |
| title_short | Optimization of recovery of esterase from Serratia marcescens using combination of the solvent impregnated resin and aqueous two-phase extraction techniques |
| title_sort | optimization of recovery of esterase from serratia marcescens using combination of the solvent impregnated resin and aqueous two-phase extraction techniques |
| url | http://psasir.upm.edu.my/id/eprint/75166/ http://psasir.upm.edu.my/id/eprint/75166/ http://psasir.upm.edu.my/id/eprint/75166/ http://psasir.upm.edu.my/id/eprint/75166/1/Optimization%2033.pdf |