Optimisation of simultaneous saccharification and fermentation (SSF) for biobutanol production using pretreated oil palm empty fruit bunch
This study was conducted in order to optimise simultaneous saccharification and fermentation (SSF) for biobutanol production from a pretreated oil palm empty fruit bunch (OPEFB) by Clostridium acetobutylicum ATCC 824. Temperature, initial pH, cellulase loading and substrate concentration were screen...
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| Format: | Article |
| Language: | English |
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M D P I AG
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/75151/ http://psasir.upm.edu.my/id/eprint/75151/1/Optimisation%20of%20simultaneous%20saccharification%20and%20fermentation.pdf |
| _version_ | 1848857617198219264 |
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| author | Md Razali, Nur Atheera Aiza Ibrahim, Mohamad Faizal Bahrin, Ezyana Abd Aziz, Suraini |
| author_facet | Md Razali, Nur Atheera Aiza Ibrahim, Mohamad Faizal Bahrin, Ezyana Abd Aziz, Suraini |
| author_sort | Md Razali, Nur Atheera Aiza |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study was conducted in order to optimise simultaneous saccharification and fermentation (SSF) for biobutanol production from a pretreated oil palm empty fruit bunch (OPEFB) by Clostridium acetobutylicum ATCC 824. Temperature, initial pH, cellulase loading and substrate concentration were screened using one factor at a time (OFAT) and further statistically optimised by central composite design (CCD) using the response surface methodology (RSM) approach. Approximately 2.47 g/L of biobutanol concentration and 0.10 g/g of biobutanol yield were obtained after being screened through OFAT with 29.55% increment (1.42 fold). The optimised conditions for SSF after CCD were: temperature of 35 °C, initial pH of 5.5, cellulase loading of 15 FPU/g-substrate and substrate concentration of 5% (w/v). This optimisation study resulted in 55.95% increment (2.14 fold) of biobutanol concentration equivalent to 3.97 g/L and biobutanol yield of 0.16 g/g. The model and optimisation design obtained from this study are important for further improvement of biobutanol production, especially in consolidated bioprocessing technology. |
| first_indexed | 2025-11-15T12:00:24Z |
| format | Article |
| id | upm-75151 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:00:24Z |
| publishDate | 2018 |
| publisher | M D P I AG |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-751512019-11-26T08:17:04Z http://psasir.upm.edu.my/id/eprint/75151/ Optimisation of simultaneous saccharification and fermentation (SSF) for biobutanol production using pretreated oil palm empty fruit bunch Md Razali, Nur Atheera Aiza Ibrahim, Mohamad Faizal Bahrin, Ezyana Abd Aziz, Suraini This study was conducted in order to optimise simultaneous saccharification and fermentation (SSF) for biobutanol production from a pretreated oil palm empty fruit bunch (OPEFB) by Clostridium acetobutylicum ATCC 824. Temperature, initial pH, cellulase loading and substrate concentration were screened using one factor at a time (OFAT) and further statistically optimised by central composite design (CCD) using the response surface methodology (RSM) approach. Approximately 2.47 g/L of biobutanol concentration and 0.10 g/g of biobutanol yield were obtained after being screened through OFAT with 29.55% increment (1.42 fold). The optimised conditions for SSF after CCD were: temperature of 35 °C, initial pH of 5.5, cellulase loading of 15 FPU/g-substrate and substrate concentration of 5% (w/v). This optimisation study resulted in 55.95% increment (2.14 fold) of biobutanol concentration equivalent to 3.97 g/L and biobutanol yield of 0.16 g/g. The model and optimisation design obtained from this study are important for further improvement of biobutanol production, especially in consolidated bioprocessing technology. M D P I AG 2018-08-03 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/75151/1/Optimisation%20of%20simultaneous%20saccharification%20and%20fermentation.pdf Md Razali, Nur Atheera Aiza and Ibrahim, Mohamad Faizal and Bahrin, Ezyana and Abd Aziz, Suraini (2018) Optimisation of simultaneous saccharification and fermentation (SSF) for biobutanol production using pretreated oil palm empty fruit bunch. Molecules, 23 (8). pp. 1-17. ISSN 1420-3049 https://www.mdpi.com/1420-3049/23/8/1944 10.3390/molecules23081944 |
| spellingShingle | Md Razali, Nur Atheera Aiza Ibrahim, Mohamad Faizal Bahrin, Ezyana Abd Aziz, Suraini Optimisation of simultaneous saccharification and fermentation (SSF) for biobutanol production using pretreated oil palm empty fruit bunch |
| title | Optimisation of simultaneous saccharification and fermentation (SSF) for biobutanol production using pretreated oil palm empty fruit bunch |
| title_full | Optimisation of simultaneous saccharification and fermentation (SSF) for biobutanol production using pretreated oil palm empty fruit bunch |
| title_fullStr | Optimisation of simultaneous saccharification and fermentation (SSF) for biobutanol production using pretreated oil palm empty fruit bunch |
| title_full_unstemmed | Optimisation of simultaneous saccharification and fermentation (SSF) for biobutanol production using pretreated oil palm empty fruit bunch |
| title_short | Optimisation of simultaneous saccharification and fermentation (SSF) for biobutanol production using pretreated oil palm empty fruit bunch |
| title_sort | optimisation of simultaneous saccharification and fermentation (ssf) for biobutanol production using pretreated oil palm empty fruit bunch |
| url | http://psasir.upm.edu.my/id/eprint/75151/ http://psasir.upm.edu.my/id/eprint/75151/ http://psasir.upm.edu.my/id/eprint/75151/ http://psasir.upm.edu.my/id/eprint/75151/1/Optimisation%20of%20simultaneous%20saccharification%20and%20fermentation.pdf |