Microtiter miniature shaken bioreactor system as a scale-down model for process development of production of therapeutic alpha-interferon2b by recombinant Escherichia coli
Background: Demand for high-throughput bioprocessing has dramatically increased especially in the biopharmaceutical industry because the technologies are of vital importance to process optimization and media development. This can be efficiently boosted by using microtiter plate (MTP) cultivation set...
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| Format: | Article |
| Language: | English |
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BioMed Central
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/74976/ http://psasir.upm.edu.my/id/eprint/74976/1/Microtiter%20miniature%20shaken%20bioreactor%20system%20as%20a%20scale-down%20model%20for%20process%20development%20of%20production%20of%20therapeutic%20alpha-interferon2b%20by%20recombinant%20Escherichia%20coli.pdf |
| _version_ | 1848857583695167488 |
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| author | Tan, Joo Shun Abbasiliasi, Sahar Kadkhodaei, Saeid Tam, Yew Joon Tang, Teck Kim Lee, Yee Ying Ariff, Arbakariya |
| author_facet | Tan, Joo Shun Abbasiliasi, Sahar Kadkhodaei, Saeid Tam, Yew Joon Tang, Teck Kim Lee, Yee Ying Ariff, Arbakariya |
| author_sort | Tan, Joo Shun |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background: Demand for high-throughput bioprocessing has dramatically increased especially in the biopharmaceutical industry because the technologies are of vital importance to process optimization and media development. This can be efficiently boosted by using microtiter plate (MTP) cultivation setup embedded into an automated liquid-handling system. The objective of this study was to establish an automated microscale method for upstream and downstream bioprocessing of α-IFN2b production by recombinant Escherichia coli. The extraction performance of α-IFN2b by osmotic shock using two different systems, automated microscale platform and manual extraction in MTP was compared. Results: The amount of α-IFN2b extracted using automated microscale platform (49.2 μg/L) was comparable to manual osmotic shock method (48.8 μg/L), but the standard deviation was 2 times lower as compared to manual osmotic shock method. Fermentation parameters in MTP involving inoculum size, agitation speed, working volume and induction profiling revealed that the fermentation conditions for the highest production of α-IFN2b (85.5 μg/L) was attained at inoculum size of 8%, working volume of 40% and agitation speed of 1000 rpm with induction at 4 h after the inoculation. Conclusion: Although the findings at MTP scale did not show perfect scalable results as compared to shake flask culture, but microscale technique development would serve as a convenient and low-cost solution in process optimization for recombinant protein. |
| first_indexed | 2025-11-15T11:59:52Z |
| format | Article |
| id | upm-74976 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:59:52Z |
| publishDate | 2018 |
| publisher | BioMed Central |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-749762020-12-13T17:45:38Z http://psasir.upm.edu.my/id/eprint/74976/ Microtiter miniature shaken bioreactor system as a scale-down model for process development of production of therapeutic alpha-interferon2b by recombinant Escherichia coli Tan, Joo Shun Abbasiliasi, Sahar Kadkhodaei, Saeid Tam, Yew Joon Tang, Teck Kim Lee, Yee Ying Ariff, Arbakariya Background: Demand for high-throughput bioprocessing has dramatically increased especially in the biopharmaceutical industry because the technologies are of vital importance to process optimization and media development. This can be efficiently boosted by using microtiter plate (MTP) cultivation setup embedded into an automated liquid-handling system. The objective of this study was to establish an automated microscale method for upstream and downstream bioprocessing of α-IFN2b production by recombinant Escherichia coli. The extraction performance of α-IFN2b by osmotic shock using two different systems, automated microscale platform and manual extraction in MTP was compared. Results: The amount of α-IFN2b extracted using automated microscale platform (49.2 μg/L) was comparable to manual osmotic shock method (48.8 μg/L), but the standard deviation was 2 times lower as compared to manual osmotic shock method. Fermentation parameters in MTP involving inoculum size, agitation speed, working volume and induction profiling revealed that the fermentation conditions for the highest production of α-IFN2b (85.5 μg/L) was attained at inoculum size of 8%, working volume of 40% and agitation speed of 1000 rpm with induction at 4 h after the inoculation. Conclusion: Although the findings at MTP scale did not show perfect scalable results as compared to shake flask culture, but microscale technique development would serve as a convenient and low-cost solution in process optimization for recombinant protein. BioMed Central 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/74976/1/Microtiter%20miniature%20shaken%20bioreactor%20system%20as%20a%20scale-down%20model%20for%20process%20development%20of%20production%20of%20therapeutic%20alpha-interferon2b%20by%20recombinant%20Escherichia%20coli.pdf Tan, Joo Shun and Abbasiliasi, Sahar and Kadkhodaei, Saeid and Tam, Yew Joon and Tang, Teck Kim and Lee, Yee Ying and Ariff, Arbakariya (2018) Microtiter miniature shaken bioreactor system as a scale-down model for process development of production of therapeutic alpha-interferon2b by recombinant Escherichia coli. BMC Microbiology, 18 (1). pp. 1-8. ISSN 1471-2180 https://pubmed.ncbi.nlm.nih.gov/29439680/ 10.1186/s12866-017-1145-9 |
| spellingShingle | Tan, Joo Shun Abbasiliasi, Sahar Kadkhodaei, Saeid Tam, Yew Joon Tang, Teck Kim Lee, Yee Ying Ariff, Arbakariya Microtiter miniature shaken bioreactor system as a scale-down model for process development of production of therapeutic alpha-interferon2b by recombinant Escherichia coli |
| title | Microtiter miniature shaken bioreactor system as a scale-down model for process development of production of therapeutic alpha-interferon2b by recombinant Escherichia coli |
| title_full | Microtiter miniature shaken bioreactor system as a scale-down model for process development of production of therapeutic alpha-interferon2b by recombinant Escherichia coli |
| title_fullStr | Microtiter miniature shaken bioreactor system as a scale-down model for process development of production of therapeutic alpha-interferon2b by recombinant Escherichia coli |
| title_full_unstemmed | Microtiter miniature shaken bioreactor system as a scale-down model for process development of production of therapeutic alpha-interferon2b by recombinant Escherichia coli |
| title_short | Microtiter miniature shaken bioreactor system as a scale-down model for process development of production of therapeutic alpha-interferon2b by recombinant Escherichia coli |
| title_sort | microtiter miniature shaken bioreactor system as a scale-down model for process development of production of therapeutic alpha-interferon2b by recombinant escherichia coli |
| url | http://psasir.upm.edu.my/id/eprint/74976/ http://psasir.upm.edu.my/id/eprint/74976/ http://psasir.upm.edu.my/id/eprint/74976/ http://psasir.upm.edu.my/id/eprint/74976/1/Microtiter%20miniature%20shaken%20bioreactor%20system%20as%20a%20scale-down%20model%20for%20process%20development%20of%20production%20of%20therapeutic%20alpha-interferon2b%20by%20recombinant%20Escherichia%20coli.pdf |