Application of polymerase chain reaction assay in detection of Leptospira icterohaemorrhagiae in experimentally infected mice
A total of 24 mice were inoculated with 1x107 of Leptospira icterohaemorrhagiae. Four mice as negative control were not inoculated. Four inoculated mice were sacrificed at each sampling day on day 1, 3, 7, 10, 14 and 21 post inoculation. Blood and kidney samples were collected and inoculated into JS...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Medwell Online
2007
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| Online Access: | http://psasir.upm.edu.my/id/eprint/7496/ |
| _version_ | 1848840610898771968 |
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| author | Omar, Sharina Bejo, Siti Khairani Bahaman, Abdul Rani |
| author_facet | Omar, Sharina Bejo, Siti Khairani Bahaman, Abdul Rani |
| author_sort | Omar, Sharina |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A total of 24 mice were inoculated with 1x107 of Leptospira icterohaemorrhagiae. Four mice as negative control were not inoculated. Four inoculated mice were sacrificed at each sampling day on day 1, 3, 7, 10, 14 and 21 post inoculation. Blood and kidney samples were collected and inoculated into JS medium for isolation of L. icterohaemorrhagiae. Polymerase Chain Reaction (PCR) assay was performed to detect the present of the organisms in blood and kidneys. All culture samples were negative for L. icterohaemorrhagiae. However, one (1/24) blood sample was positive by PCR. It is apparent that detection of leptospires in blood with PCR is a promising approach for early diagnosis of leptospirosis. |
| first_indexed | 2025-11-15T07:30:05Z |
| format | Article |
| id | upm-7496 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:30:05Z |
| publishDate | 2007 |
| publisher | Medwell Online |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-74962010-07-16T04:17:33Z http://psasir.upm.edu.my/id/eprint/7496/ Application of polymerase chain reaction assay in detection of Leptospira icterohaemorrhagiae in experimentally infected mice Omar, Sharina Bejo, Siti Khairani Bahaman, Abdul Rani A total of 24 mice were inoculated with 1x107 of Leptospira icterohaemorrhagiae. Four mice as negative control were not inoculated. Four inoculated mice were sacrificed at each sampling day on day 1, 3, 7, 10, 14 and 21 post inoculation. Blood and kidney samples were collected and inoculated into JS medium for isolation of L. icterohaemorrhagiae. Polymerase Chain Reaction (PCR) assay was performed to detect the present of the organisms in blood and kidneys. All culture samples were negative for L. icterohaemorrhagiae. However, one (1/24) blood sample was positive by PCR. It is apparent that detection of leptospires in blood with PCR is a promising approach for early diagnosis of leptospirosis. Medwell Online 2007 Article PeerReviewed Omar, Sharina and Bejo, Siti Khairani and Bahaman, Abdul Rani (2007) Application of polymerase chain reaction assay in detection of Leptospira icterohaemorrhagiae in experimentally infected mice. Journal of Animal and Veterinary Advances, 6 (10). pp. 1197-1198. ISSN 1680-5593 http://dx.doi.org/10.3923/javaa.2007.1197.1198 English |
| spellingShingle | Omar, Sharina Bejo, Siti Khairani Bahaman, Abdul Rani Application of polymerase chain reaction assay in detection of Leptospira icterohaemorrhagiae in experimentally infected mice |
| title | Application of polymerase chain reaction assay in detection of Leptospira icterohaemorrhagiae in experimentally infected mice |
| title_full | Application of polymerase chain reaction assay in detection of Leptospira icterohaemorrhagiae in experimentally infected mice |
| title_fullStr | Application of polymerase chain reaction assay in detection of Leptospira icterohaemorrhagiae in experimentally infected mice |
| title_full_unstemmed | Application of polymerase chain reaction assay in detection of Leptospira icterohaemorrhagiae in experimentally infected mice |
| title_short | Application of polymerase chain reaction assay in detection of Leptospira icterohaemorrhagiae in experimentally infected mice |
| title_sort | application of polymerase chain reaction assay in detection of leptospira icterohaemorrhagiae in experimentally infected mice |
| url | http://psasir.upm.edu.my/id/eprint/7496/ http://psasir.upm.edu.my/id/eprint/7496/ |