Introgression of root trait genes for drought tolerance to a Malaysian rice variety by marker-assisted backcross breeding

Drought gives a huge impact in declining rice yield. To overcome this problem, rice variety with ability to withstand limited water availability need to be developed. The study was conducted to introgress root trait genes from aerobic rice variety, AERON1, to a high yielding rice variety of Malaysia...

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Main Authors: Ab Jalil, Masitah, Juraimi, Abdul Shukor, Yusop, Mohd Rafii, Uddin, Md Kamal, Hakim, Md Abdul
Format: Article
Language:English
Published: Friends Science Publishers 2018
Online Access:http://psasir.upm.edu.my/id/eprint/74729/
http://psasir.upm.edu.my/id/eprint/74729/1/Introgression%20of%20root%20trait%20genes%20for%20drought%20tolerance%20to%20a%20Malaysian%20rice%20variety%20by%20marker-assisted%20backcross%20breeding.pdf
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author Ab Jalil, Masitah
Juraimi, Abdul Shukor
Yusop, Mohd Rafii
Uddin, Md Kamal
Hakim, Md Abdul
author_facet Ab Jalil, Masitah
Juraimi, Abdul Shukor
Yusop, Mohd Rafii
Uddin, Md Kamal
Hakim, Md Abdul
author_sort Ab Jalil, Masitah
building UPM Institutional Repository
collection Online Access
description Drought gives a huge impact in declining rice yield. To overcome this problem, rice variety with ability to withstand limited water availability need to be developed. The study was conducted to introgress root trait genes from aerobic rice variety, AERON1, to a high yielding rice variety of Malaysia, MRQ74, through marker-assisted backcross breeding (MABC). Two foreground markers, RM242 and RM263, and 57 background markers were used along the breeding program. The Chi-square (χ²) analysis showed a good fit to the expected segregation ratio (1:1) for a single dominant gene model (d.f. =1, P > 0.05) for both foreground markers. The Chi-square values in BC1F1 and BC2F1, for RM242 and RM263 were χ²=3.1 and χ²=0.03, and χ²=1.01 and χ²=0.22, respectively. The recurrent parent genome (RPG) percentage for BC1F1 and BC2F1 were 71.0% and 89.6%, respectively. Eventually, six best rice lines in BC2F1 population were identified to carry root trait genes for drought tolerance. The lines were closely related to the recurrent parent by having the highest recovery percentage and approximately similar phenotypic appearance. Hence, the development of drought tolerance rice lines through marker-assisted backcross breeding was believed to help the researchers in understanding the importance of molecular markers in enhancing the ability of rice crop towards biotic and abiotic constraints and accelerating the development of new varieties.
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spelling upm-747292019-11-27T08:04:27Z http://psasir.upm.edu.my/id/eprint/74729/ Introgression of root trait genes for drought tolerance to a Malaysian rice variety by marker-assisted backcross breeding Ab Jalil, Masitah Juraimi, Abdul Shukor Yusop, Mohd Rafii Uddin, Md Kamal Hakim, Md Abdul Drought gives a huge impact in declining rice yield. To overcome this problem, rice variety with ability to withstand limited water availability need to be developed. The study was conducted to introgress root trait genes from aerobic rice variety, AERON1, to a high yielding rice variety of Malaysia, MRQ74, through marker-assisted backcross breeding (MABC). Two foreground markers, RM242 and RM263, and 57 background markers were used along the breeding program. The Chi-square (χ²) analysis showed a good fit to the expected segregation ratio (1:1) for a single dominant gene model (d.f. =1, P > 0.05) for both foreground markers. The Chi-square values in BC1F1 and BC2F1, for RM242 and RM263 were χ²=3.1 and χ²=0.03, and χ²=1.01 and χ²=0.22, respectively. The recurrent parent genome (RPG) percentage for BC1F1 and BC2F1 were 71.0% and 89.6%, respectively. Eventually, six best rice lines in BC2F1 population were identified to carry root trait genes for drought tolerance. The lines were closely related to the recurrent parent by having the highest recovery percentage and approximately similar phenotypic appearance. Hence, the development of drought tolerance rice lines through marker-assisted backcross breeding was believed to help the researchers in understanding the importance of molecular markers in enhancing the ability of rice crop towards biotic and abiotic constraints and accelerating the development of new varieties. Friends Science Publishers 2018-01 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/74729/1/Introgression%20of%20root%20trait%20genes%20for%20drought%20tolerance%20to%20a%20Malaysian%20rice%20variety%20by%20marker-assisted%20backcross%20breeding.pdf Ab Jalil, Masitah and Juraimi, Abdul Shukor and Yusop, Mohd Rafii and Uddin, Md Kamal and Hakim, Md Abdul (2018) Introgression of root trait genes for drought tolerance to a Malaysian rice variety by marker-assisted backcross breeding. International Journal of Agriculture and Biology, 20 (1). 119 - 126. ISSN 1560-8530; ESSN: 1814-9596 https://fspublishers.org/Issue.php?y=2018&v_no=20&categoryID=150 10.17957/IJAB/15.0462
spellingShingle Ab Jalil, Masitah
Juraimi, Abdul Shukor
Yusop, Mohd Rafii
Uddin, Md Kamal
Hakim, Md Abdul
Introgression of root trait genes for drought tolerance to a Malaysian rice variety by marker-assisted backcross breeding
title Introgression of root trait genes for drought tolerance to a Malaysian rice variety by marker-assisted backcross breeding
title_full Introgression of root trait genes for drought tolerance to a Malaysian rice variety by marker-assisted backcross breeding
title_fullStr Introgression of root trait genes for drought tolerance to a Malaysian rice variety by marker-assisted backcross breeding
title_full_unstemmed Introgression of root trait genes for drought tolerance to a Malaysian rice variety by marker-assisted backcross breeding
title_short Introgression of root trait genes for drought tolerance to a Malaysian rice variety by marker-assisted backcross breeding
title_sort introgression of root trait genes for drought tolerance to a malaysian rice variety by marker-assisted backcross breeding
url http://psasir.upm.edu.my/id/eprint/74729/
http://psasir.upm.edu.my/id/eprint/74729/
http://psasir.upm.edu.my/id/eprint/74729/
http://psasir.upm.edu.my/id/eprint/74729/1/Introgression%20of%20root%20trait%20genes%20for%20drought%20tolerance%20to%20a%20Malaysian%20rice%20variety%20by%20marker-assisted%20backcross%20breeding.pdf