Inhibition and kinetic studies of lignin degrading enzymes of Ganoderma boninense by naturally occurring phenolic compounds
Aim Lignolytic (lignin degrading) enzyme, from oil palm pathogen Ganoderma boninense Pat. (Syn G. orbiforme (Ryvarden)), is involved in the detoxification and the degradation of lignin in the oil palm and is the rate‐limiting step in the infection process of this fungus. Active inhibition of lignin...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/74686/ http://psasir.upm.edu.my/id/eprint/74686/1/Inhibition%20and%20kinetic.pdf |
| _version_ | 1848857538212134912 |
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| author | A., Surendran Y., Siddiqui H. M., Saud N. S., Ali S., Manickam |
| author_facet | A., Surendran Y., Siddiqui H. M., Saud N. S., Ali S., Manickam |
| author_sort | A., Surendran |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Aim
Lignolytic (lignin degrading) enzyme, from oil palm pathogen Ganoderma boninense Pat. (Syn G. orbiforme (Ryvarden)), is involved in the detoxification and the degradation of lignin in the oil palm and is the rate‐limiting step in the infection process of this fungus. Active inhibition of lignin‐degrading enzymes secreted by G. boninense by various naturally occurring phenolic compounds and estimation of efficiency on pathogen suppression was aimed at.
Methods and Results
In our work, 10 naturally occurring phenolic compounds were evaluated for their inhibitory potential towards the lignolytic enzymes of G. boninense. Additionally, the lignin‐degrading enzymes were characterized. Most of the peholic compounds exhibited an uncompetitive inhibition towards the lignin‐degrading enzymes. Benzoic acid was the superior inhibitor to the production of lignin‐degrading enzymes, when compared between the 10 phenolic compounds. The inhibitory potential of the phenolic compounds towards the lignin‐degrading enzymes are higher than that of the conventional metal ion inhibitor. The lignin‐degrading enzymes were stable in a wide range of pH but were sensitive to higher temperature.
Conclusion
The study demonstrated the inhibitor potential of 10 naturally occurring phenolic compounds towards the lignin‐degrading enzymes of G. boninense with different efficacies.
Significance and Impact of the Study
The study has shed a light towards a new management strategy to control basal stem rot disease in oil palm. It serves as a replacement for the existing chemical control. |
| first_indexed | 2025-11-15T11:59:08Z |
| format | Article |
| id | upm-74686 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:59:08Z |
| publishDate | 2018 |
| publisher | Wiley |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-746862020-02-27T03:20:20Z http://psasir.upm.edu.my/id/eprint/74686/ Inhibition and kinetic studies of lignin degrading enzymes of Ganoderma boninense by naturally occurring phenolic compounds A., Surendran Y., Siddiqui H. M., Saud N. S., Ali S., Manickam Aim Lignolytic (lignin degrading) enzyme, from oil palm pathogen Ganoderma boninense Pat. (Syn G. orbiforme (Ryvarden)), is involved in the detoxification and the degradation of lignin in the oil palm and is the rate‐limiting step in the infection process of this fungus. Active inhibition of lignin‐degrading enzymes secreted by G. boninense by various naturally occurring phenolic compounds and estimation of efficiency on pathogen suppression was aimed at. Methods and Results In our work, 10 naturally occurring phenolic compounds were evaluated for their inhibitory potential towards the lignolytic enzymes of G. boninense. Additionally, the lignin‐degrading enzymes were characterized. Most of the peholic compounds exhibited an uncompetitive inhibition towards the lignin‐degrading enzymes. Benzoic acid was the superior inhibitor to the production of lignin‐degrading enzymes, when compared between the 10 phenolic compounds. The inhibitory potential of the phenolic compounds towards the lignin‐degrading enzymes are higher than that of the conventional metal ion inhibitor. The lignin‐degrading enzymes were stable in a wide range of pH but were sensitive to higher temperature. Conclusion The study demonstrated the inhibitor potential of 10 naturally occurring phenolic compounds towards the lignin‐degrading enzymes of G. boninense with different efficacies. Significance and Impact of the Study The study has shed a light towards a new management strategy to control basal stem rot disease in oil palm. It serves as a replacement for the existing chemical control. Wiley 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/74686/1/Inhibition%20and%20kinetic.pdf A., Surendran and Y., Siddiqui and H. M., Saud and N. S., Ali and S., Manickam (2018) Inhibition and kinetic studies of lignin degrading enzymes of Ganoderma boninense by naturally occurring phenolic compounds. Journal of Applied Microbiology, 125 (3). 876 - 887. ISSN 1364-5072; ESSN: 1365-2672 10.1111/jam.13922 |
| spellingShingle | A., Surendran Y., Siddiqui H. M., Saud N. S., Ali S., Manickam Inhibition and kinetic studies of lignin degrading enzymes of Ganoderma boninense by naturally occurring phenolic compounds |
| title | Inhibition and kinetic studies of lignin degrading enzymes of Ganoderma boninense by naturally occurring phenolic compounds |
| title_full | Inhibition and kinetic studies of lignin degrading enzymes of Ganoderma boninense by naturally occurring phenolic compounds |
| title_fullStr | Inhibition and kinetic studies of lignin degrading enzymes of Ganoderma boninense by naturally occurring phenolic compounds |
| title_full_unstemmed | Inhibition and kinetic studies of lignin degrading enzymes of Ganoderma boninense by naturally occurring phenolic compounds |
| title_short | Inhibition and kinetic studies of lignin degrading enzymes of Ganoderma boninense by naturally occurring phenolic compounds |
| title_sort | inhibition and kinetic studies of lignin degrading enzymes of ganoderma boninense by naturally occurring phenolic compounds |
| url | http://psasir.upm.edu.my/id/eprint/74686/ http://psasir.upm.edu.my/id/eprint/74686/ http://psasir.upm.edu.my/id/eprint/74686/1/Inhibition%20and%20kinetic.pdf |