Human mesenchymal stem cells expressing erythropoietin enhance survivability of retinal neurons against oxidative stress : an in-vitro study
Retinal degeneration is a prominent feature in ocular disorders. In exploring possible treatments, Mesenchymal Stem Cells (MSCs) have been recognized to yield therapeutic role for retinal degenerative diseases. Studies have also displayed that erythropoietin (EPO) administration into degenerative re...
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| Format: | Article |
| Language: | English |
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Frontiers Media
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/74524/ http://psasir.upm.edu.my/id/eprint/74524/1/Human%20mesenchymal%20stem.pdf |
| _version_ | 1848857513009610752 |
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| author | Suet Lee, Shirley Ding Kumar, Suresh Ali Khan, Mohammed Safwan Pooi, Ling Mok |
| author_facet | Suet Lee, Shirley Ding Kumar, Suresh Ali Khan, Mohammed Safwan Pooi, Ling Mok |
| author_sort | Suet Lee, Shirley Ding |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Retinal degeneration is a prominent feature in ocular disorders. In exploring possible treatments, Mesenchymal Stem Cells (MSCs) have been recognized to yield therapeutic role for retinal degenerative diseases. Studies have also displayed that erythropoietin (EPO) administration into degenerative retina models confers significant neuroprotective actions in limiting pathological cell death. In this study, we aimed to use MSCs to deliver EPO and to evaluate the ability of EPO to rescue retinal neurons from dying upon reactive oxidative stress induction. We derived human MSCs from Wharton's jelly (hWJMSCs) of the umbilical cord and cells were transduced with lentivirus particles encoding EPO and a reporter gene of green fluorescent protein (GFP). The supernatants of both transduced and non-transduced cells were collected and used as a pre-conditioning medium for Y79 retinoblastoma cells (retinal neuron cell line) following exposure to glutamate induction. Retinal cells exposed to glutamate showed reduced mitochondrial depolarization and enhanced improvement in cell viability when incubated with pre-conditioned media of transduced cells. Our results established a proof-of-concept that MSCs could be used as a candidate for the delivery of EPO therapeutic gene in the treatment of retinal degenerations. |
| first_indexed | 2025-11-15T11:58:44Z |
| format | Article |
| id | upm-74524 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:58:44Z |
| publishDate | 2018 |
| publisher | Frontiers Media |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-745242020-03-17T08:09:42Z http://psasir.upm.edu.my/id/eprint/74524/ Human mesenchymal stem cells expressing erythropoietin enhance survivability of retinal neurons against oxidative stress : an in-vitro study Suet Lee, Shirley Ding Kumar, Suresh Ali Khan, Mohammed Safwan Pooi, Ling Mok Retinal degeneration is a prominent feature in ocular disorders. In exploring possible treatments, Mesenchymal Stem Cells (MSCs) have been recognized to yield therapeutic role for retinal degenerative diseases. Studies have also displayed that erythropoietin (EPO) administration into degenerative retina models confers significant neuroprotective actions in limiting pathological cell death. In this study, we aimed to use MSCs to deliver EPO and to evaluate the ability of EPO to rescue retinal neurons from dying upon reactive oxidative stress induction. We derived human MSCs from Wharton's jelly (hWJMSCs) of the umbilical cord and cells were transduced with lentivirus particles encoding EPO and a reporter gene of green fluorescent protein (GFP). The supernatants of both transduced and non-transduced cells were collected and used as a pre-conditioning medium for Y79 retinoblastoma cells (retinal neuron cell line) following exposure to glutamate induction. Retinal cells exposed to glutamate showed reduced mitochondrial depolarization and enhanced improvement in cell viability when incubated with pre-conditioned media of transduced cells. Our results established a proof-of-concept that MSCs could be used as a candidate for the delivery of EPO therapeutic gene in the treatment of retinal degenerations. Frontiers Media 2018-07-31 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/74524/1/Human%20mesenchymal%20stem.pdf Suet Lee, Shirley Ding and Kumar, Suresh and Ali Khan, Mohammed Safwan and Pooi, Ling Mok (2018) Human mesenchymal stem cells expressing erythropoietin enhance survivability of retinal neurons against oxidative stress : an in-vitro study. Frontiers in Cellular Neuroscience, - (-). pp. 1-12. ISSN 1662-5102 10.3389/fncel.2018.00190 |
| spellingShingle | Suet Lee, Shirley Ding Kumar, Suresh Ali Khan, Mohammed Safwan Pooi, Ling Mok Human mesenchymal stem cells expressing erythropoietin enhance survivability of retinal neurons against oxidative stress : an in-vitro study |
| title | Human mesenchymal stem cells expressing erythropoietin enhance survivability of retinal neurons against oxidative stress : an in-vitro study |
| title_full | Human mesenchymal stem cells expressing erythropoietin enhance survivability of retinal neurons against oxidative stress : an in-vitro study |
| title_fullStr | Human mesenchymal stem cells expressing erythropoietin enhance survivability of retinal neurons against oxidative stress : an in-vitro study |
| title_full_unstemmed | Human mesenchymal stem cells expressing erythropoietin enhance survivability of retinal neurons against oxidative stress : an in-vitro study |
| title_short | Human mesenchymal stem cells expressing erythropoietin enhance survivability of retinal neurons against oxidative stress : an in-vitro study |
| title_sort | human mesenchymal stem cells expressing erythropoietin enhance survivability of retinal neurons against oxidative stress : an in-vitro study |
| url | http://psasir.upm.edu.my/id/eprint/74524/ http://psasir.upm.edu.my/id/eprint/74524/ http://psasir.upm.edu.my/id/eprint/74524/1/Human%20mesenchymal%20stem.pdf |