High angiotensin-I converting enzyme (ACE) inhibitory activity of Alcalase-digested green soybean (Glycine max) hydrolysates
As a protein-rich, underutilized crop, green soybean could be exploited to produce hydrolysates containing angiotensin-I converting enzyme (ACE) inhibitory peptides. Defatted green soybean was hydrolyzed using four different food-grade proteases (Alcalase, Papain, Flavourzyme and Bromelain) and thei...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/74503/ http://psasir.upm.edu.my/id/eprint/74503/1/High%20angiotensin.pdf |
| _version_ | 1848857509721276416 |
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| author | Hanafi, Mohamad Ariff Hashim, Siti Nadia Chay, Shyan Yea Ebrahimpour, Afshin Zarei, Mohammad Muhammad, Kharidah Abdul Hamid, Azizah Saari, Nazamid |
| author_facet | Hanafi, Mohamad Ariff Hashim, Siti Nadia Chay, Shyan Yea Ebrahimpour, Afshin Zarei, Mohammad Muhammad, Kharidah Abdul Hamid, Azizah Saari, Nazamid |
| author_sort | Hanafi, Mohamad Ariff |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | As a protein-rich, underutilized crop, green soybean could be exploited to produce hydrolysates containing angiotensin-I converting enzyme (ACE) inhibitory peptides. Defatted green soybean was hydrolyzed using four different food-grade proteases (Alcalase, Papain, Flavourzyme and Bromelain) and their ACE inhibitory activities were evaluated. The Alcalase-generated green soybean hydrolysate showed the highest ACE inhibitory activity (IC₅₀: 0.14 mg/mL at 6 h hydrolysis time) followed by Papain (IC₅₀: 0.20 mg/mL at 5 h hydrolysis time), Bromelain (IC₅₀: 0.36 mg/mL at 6 h hydrolysis time) and Flavourzyme (IC₅: 1.14 mg/mL at 6 h hydrolysis time) hydrolysates. The Alcalase-generated hydrolysate was profiled based on its hydrophobicity and isoelectric point using reversed phase high performance liquid chromatography (RP-HPLC) and isoelectric point focusing (IEF) fractionators. The Alcalase-generated green soybean hydrolysate comprising of peptides EAQRLLF, PSLRSYLAE, PDRSIHGRQLAE, FITAFR and RGQVLS, revealed the highest ACE inhibitory activity of 94.19%, 99.31%, 92.92%, 101.51% and 90.40%, respectively, while their IC₅₀ values were 878 μM, 532 μM, 1552 μM, 1342 μM and 993 μM, respectively. It can be concluded that Alcalase-digested green soybean hydrolysates could be exploited as a source of peptides to be incorporated into functional foods with antihypertensive activity. |
| first_indexed | 2025-11-15T11:58:41Z |
| format | Article |
| id | upm-74503 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:58:41Z |
| publishDate | 2018 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-745032020-10-17T21:16:50Z http://psasir.upm.edu.my/id/eprint/74503/ High angiotensin-I converting enzyme (ACE) inhibitory activity of Alcalase-digested green soybean (Glycine max) hydrolysates Hanafi, Mohamad Ariff Hashim, Siti Nadia Chay, Shyan Yea Ebrahimpour, Afshin Zarei, Mohammad Muhammad, Kharidah Abdul Hamid, Azizah Saari, Nazamid As a protein-rich, underutilized crop, green soybean could be exploited to produce hydrolysates containing angiotensin-I converting enzyme (ACE) inhibitory peptides. Defatted green soybean was hydrolyzed using four different food-grade proteases (Alcalase, Papain, Flavourzyme and Bromelain) and their ACE inhibitory activities were evaluated. The Alcalase-generated green soybean hydrolysate showed the highest ACE inhibitory activity (IC₅₀: 0.14 mg/mL at 6 h hydrolysis time) followed by Papain (IC₅₀: 0.20 mg/mL at 5 h hydrolysis time), Bromelain (IC₅₀: 0.36 mg/mL at 6 h hydrolysis time) and Flavourzyme (IC₅: 1.14 mg/mL at 6 h hydrolysis time) hydrolysates. The Alcalase-generated hydrolysate was profiled based on its hydrophobicity and isoelectric point using reversed phase high performance liquid chromatography (RP-HPLC) and isoelectric point focusing (IEF) fractionators. The Alcalase-generated green soybean hydrolysate comprising of peptides EAQRLLF, PSLRSYLAE, PDRSIHGRQLAE, FITAFR and RGQVLS, revealed the highest ACE inhibitory activity of 94.19%, 99.31%, 92.92%, 101.51% and 90.40%, respectively, while their IC₅₀ values were 878 μM, 532 μM, 1552 μM, 1342 μM and 993 μM, respectively. It can be concluded that Alcalase-digested green soybean hydrolysates could be exploited as a source of peptides to be incorporated into functional foods with antihypertensive activity. Elsevier 2018-04 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/74503/1/High%20angiotensin.pdf Hanafi, Mohamad Ariff and Hashim, Siti Nadia and Chay, Shyan Yea and Ebrahimpour, Afshin and Zarei, Mohammad and Muhammad, Kharidah and Abdul Hamid, Azizah and Saari, Nazamid (2018) High angiotensin-I converting enzyme (ACE) inhibitory activity of Alcalase-digested green soybean (Glycine max) hydrolysates. Food Research International, 106. 589 - 597. ISSN 0963-9969 https://www.sciencedirect.com/science/article/abs/pii/S0963996918300309 10.1016/j.foodres.2018.01.030 |
| spellingShingle | Hanafi, Mohamad Ariff Hashim, Siti Nadia Chay, Shyan Yea Ebrahimpour, Afshin Zarei, Mohammad Muhammad, Kharidah Abdul Hamid, Azizah Saari, Nazamid High angiotensin-I converting enzyme (ACE) inhibitory activity of Alcalase-digested green soybean (Glycine max) hydrolysates |
| title | High angiotensin-I converting enzyme (ACE) inhibitory activity of Alcalase-digested green soybean (Glycine max) hydrolysates |
| title_full | High angiotensin-I converting enzyme (ACE) inhibitory activity of Alcalase-digested green soybean (Glycine max) hydrolysates |
| title_fullStr | High angiotensin-I converting enzyme (ACE) inhibitory activity of Alcalase-digested green soybean (Glycine max) hydrolysates |
| title_full_unstemmed | High angiotensin-I converting enzyme (ACE) inhibitory activity of Alcalase-digested green soybean (Glycine max) hydrolysates |
| title_short | High angiotensin-I converting enzyme (ACE) inhibitory activity of Alcalase-digested green soybean (Glycine max) hydrolysates |
| title_sort | high angiotensin-i converting enzyme (ace) inhibitory activity of alcalase-digested green soybean (glycine max) hydrolysates |
| url | http://psasir.upm.edu.my/id/eprint/74503/ http://psasir.upm.edu.my/id/eprint/74503/ http://psasir.upm.edu.my/id/eprint/74503/ http://psasir.upm.edu.my/id/eprint/74503/1/High%20angiotensin.pdf |