Targeted delivery of cell penetrating peptide virus-like nanoparticles to skin cancer cells
Skin cancer or cutaneous carcinoma, is a pre-eminent global public health problem with no signs of plateauing in its incidence. As the most common treatments for skin cancer, surgical resection inevitably damages a patient's appearance, and chemotherapy has many side effects. Thus, the main aim...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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http://www.nature.com/
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/74081/ http://psasir.upm.edu.my/id/eprint/74081/1/Targeted%20delivery%20of%20cell%20penetrating%20peptide%20virus-like%20nanoparticles%20to%20skin%20cancer%20cells.pdf |
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| author | Gan, Bee Koon Yong, Chean Yeah Ho, Kok Lian Omar, Abdul Rahman Mohammed Alitheen, Noorjahan Banu Tan, Wen Siang |
| author_facet | Gan, Bee Koon Yong, Chean Yeah Ho, Kok Lian Omar, Abdul Rahman Mohammed Alitheen, Noorjahan Banu Tan, Wen Siang |
| author_sort | Gan, Bee Koon |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Skin cancer or cutaneous carcinoma, is a pre-eminent global public health problem with no signs of plateauing in its incidence. As the most common treatments for skin cancer, surgical resection inevitably damages a patient's appearance, and chemotherapy has many side effects. Thus, the main aim of this study was to screen for a cell penetrating peptide (CPP) for the development of a targeting vector for skin cancer. In this study, we identified a CPP with the sequence NRPDSAQFWLHH from a phage displayed peptide library. This CPP targeted the human squamous carcinoma A431 cells through an interaction with the epidermal growth factor receptor (EGFr). Methyl-β-cyclodextrin (MβCD) and chlorpromazine hydrochloride (CPZ) inhibited the internalisation of the CPP into the A431 cells, suggesting the peptide entered the cells via clathrin-dependent endocytosis. The CPP displayed on hepatitis B virus-like nanoparticles (VLNPs) via the nanoglue successfully delivered the nanoparticles into A431 cells. The present study demonstrated that the novel CPP can serve as a ligand to target and deliver VLNPs into skin cancer cells. |
| first_indexed | 2025-11-15T11:57:25Z |
| format | Article |
| id | upm-74081 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:57:25Z |
| publishDate | 2018 |
| publisher | http://www.nature.com/ |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-740812020-04-26T22:56:27Z http://psasir.upm.edu.my/id/eprint/74081/ Targeted delivery of cell penetrating peptide virus-like nanoparticles to skin cancer cells Gan, Bee Koon Yong, Chean Yeah Ho, Kok Lian Omar, Abdul Rahman Mohammed Alitheen, Noorjahan Banu Tan, Wen Siang Skin cancer or cutaneous carcinoma, is a pre-eminent global public health problem with no signs of plateauing in its incidence. As the most common treatments for skin cancer, surgical resection inevitably damages a patient's appearance, and chemotherapy has many side effects. Thus, the main aim of this study was to screen for a cell penetrating peptide (CPP) for the development of a targeting vector for skin cancer. In this study, we identified a CPP with the sequence NRPDSAQFWLHH from a phage displayed peptide library. This CPP targeted the human squamous carcinoma A431 cells through an interaction with the epidermal growth factor receptor (EGFr). Methyl-β-cyclodextrin (MβCD) and chlorpromazine hydrochloride (CPZ) inhibited the internalisation of the CPP into the A431 cells, suggesting the peptide entered the cells via clathrin-dependent endocytosis. The CPP displayed on hepatitis B virus-like nanoparticles (VLNPs) via the nanoglue successfully delivered the nanoparticles into A431 cells. The present study demonstrated that the novel CPP can serve as a ligand to target and deliver VLNPs into skin cancer cells. http://www.nature.com/ 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/74081/1/Targeted%20delivery%20of%20cell%20penetrating%20peptide%20virus-like%20nanoparticles%20to%20skin%20cancer%20cells.pdf Gan, Bee Koon and Yong, Chean Yeah and Ho, Kok Lian and Omar, Abdul Rahman and Mohammed Alitheen, Noorjahan Banu and Tan, Wen Siang (2018) Targeted delivery of cell penetrating peptide virus-like nanoparticles to skin cancer cells. Scientific Reports, 8. art. no. 8499. pp. 1-13. ISSN ESSN: 2045-2322 10.1038/s41598-018-26749-y |
| spellingShingle | Gan, Bee Koon Yong, Chean Yeah Ho, Kok Lian Omar, Abdul Rahman Mohammed Alitheen, Noorjahan Banu Tan, Wen Siang Targeted delivery of cell penetrating peptide virus-like nanoparticles to skin cancer cells |
| title | Targeted delivery of cell penetrating peptide virus-like nanoparticles to skin cancer cells |
| title_full | Targeted delivery of cell penetrating peptide virus-like nanoparticles to skin cancer cells |
| title_fullStr | Targeted delivery of cell penetrating peptide virus-like nanoparticles to skin cancer cells |
| title_full_unstemmed | Targeted delivery of cell penetrating peptide virus-like nanoparticles to skin cancer cells |
| title_short | Targeted delivery of cell penetrating peptide virus-like nanoparticles to skin cancer cells |
| title_sort | targeted delivery of cell penetrating peptide virus-like nanoparticles to skin cancer cells |
| url | http://psasir.upm.edu.my/id/eprint/74081/ http://psasir.upm.edu.my/id/eprint/74081/ http://psasir.upm.edu.my/id/eprint/74081/1/Targeted%20delivery%20of%20cell%20penetrating%20peptide%20virus-like%20nanoparticles%20to%20skin%20cancer%20cells.pdf |