Structural, optical and sensing properties of CdS-NH2GO thin film as a dengue virus E-protein sensing material
Semiconductor nanoparticles, cadmium sulfide quantum dots composited with amine functionalized graphene oxide (CdS-NH2GO) were functionalized with specific monoclonal antibodies (IgM) based on surface plasmon resonance (SPR) optical sensor for the detection of dengue virus (DENV) E-protein. The SPR...
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| Format: | Article |
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Elsevier
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/73990/ |
| _version_ | 1848857410489286656 |
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| author | Sheh Omar, Nur Alia Yap, Wing Fen Abdullah, Jaafar Mat Zaid, Mohd Hazani Mahdi, Mohd Adzir |
| author_facet | Sheh Omar, Nur Alia Yap, Wing Fen Abdullah, Jaafar Mat Zaid, Mohd Hazani Mahdi, Mohd Adzir |
| author_sort | Sheh Omar, Nur Alia |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Semiconductor nanoparticles, cadmium sulfide quantum dots composited with amine functionalized graphene oxide (CdS-NH2GO) were functionalized with specific monoclonal antibodies (IgM) based on surface plasmon resonance (SPR) optical sensor for the detection of dengue virus (DENV) E-protein. The SPR results suggested that DENV E-protein can be detected in the range 0.0001–10 nM with the binding affinity of 486.54 nM−1. The successful binding between antibodies and antigens in an immunoassay sensor was further confirmed by Raman, UV–vis spectroscopy and atomic force microscopy (AFM). The Raman studies showed the depletion of protein molecules in the region of 120–1000 cm−1, which gives an evidence for the binding of DENV E-proteins to the antibodies. From UV–vis spectra, the absorption band was red shifted upon binding of DENV E-protein. The AFM measurement further confirmed the interaction occurred between DENV E-protein and the Au/CdS-NH2GO/IgM sensor surface. |
| first_indexed | 2025-11-15T11:57:07Z |
| format | Article |
| id | upm-73990 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T11:57:07Z |
| publishDate | 2018 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-739902023-06-19T06:55:59Z http://psasir.upm.edu.my/id/eprint/73990/ Structural, optical and sensing properties of CdS-NH2GO thin film as a dengue virus E-protein sensing material Sheh Omar, Nur Alia Yap, Wing Fen Abdullah, Jaafar Mat Zaid, Mohd Hazani Mahdi, Mohd Adzir Semiconductor nanoparticles, cadmium sulfide quantum dots composited with amine functionalized graphene oxide (CdS-NH2GO) were functionalized with specific monoclonal antibodies (IgM) based on surface plasmon resonance (SPR) optical sensor for the detection of dengue virus (DENV) E-protein. The SPR results suggested that DENV E-protein can be detected in the range 0.0001–10 nM with the binding affinity of 486.54 nM−1. The successful binding between antibodies and antigens in an immunoassay sensor was further confirmed by Raman, UV–vis spectroscopy and atomic force microscopy (AFM). The Raman studies showed the depletion of protein molecules in the region of 120–1000 cm−1, which gives an evidence for the binding of DENV E-proteins to the antibodies. From UV–vis spectra, the absorption band was red shifted upon binding of DENV E-protein. The AFM measurement further confirmed the interaction occurred between DENV E-protein and the Au/CdS-NH2GO/IgM sensor surface. Elsevier 2018-10 Article PeerReviewed Sheh Omar, Nur Alia and Yap, Wing Fen and Abdullah, Jaafar and Mat Zaid, Mohd Hazani and Mahdi, Mohd Adzir (2018) Structural, optical and sensing properties of CdS-NH2GO thin film as a dengue virus E-protein sensing material. Optik, 171. 934 - 940. ISSN 0030-4026; ESSN: 1618-1336 https://www.sciencedirect.com/journal/optik 10.1016/j.ijleo.2018.07.001 |
| spellingShingle | Sheh Omar, Nur Alia Yap, Wing Fen Abdullah, Jaafar Mat Zaid, Mohd Hazani Mahdi, Mohd Adzir Structural, optical and sensing properties of CdS-NH2GO thin film as a dengue virus E-protein sensing material |
| title | Structural, optical and sensing properties of CdS-NH2GO thin film as a dengue virus E-protein sensing material |
| title_full | Structural, optical and sensing properties of CdS-NH2GO thin film as a dengue virus E-protein sensing material |
| title_fullStr | Structural, optical and sensing properties of CdS-NH2GO thin film as a dengue virus E-protein sensing material |
| title_full_unstemmed | Structural, optical and sensing properties of CdS-NH2GO thin film as a dengue virus E-protein sensing material |
| title_short | Structural, optical and sensing properties of CdS-NH2GO thin film as a dengue virus E-protein sensing material |
| title_sort | structural, optical and sensing properties of cds-nh2go thin film as a dengue virus e-protein sensing material |
| url | http://psasir.upm.edu.my/id/eprint/73990/ http://psasir.upm.edu.my/id/eprint/73990/ http://psasir.upm.edu.my/id/eprint/73990/ |