Sensitive leptospira DNA detection using tapered optical fiber sensor
This paper presents the development of tapered optical fiber sensor to detect a specific Leptospira bacteria DNA. The bacteria causes Leptospirosis, a deadly disease but with common early flu-like symptoms. Optical single mode fiber (SMF) of 125 μm diameter is tapered to produce 12 μm waist diameter...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH Verlag
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/73862/ http://psasir.upm.edu.my/id/eprint/73862/1/Sensitive%20leptospira%20DNA%20detection%20using%20tapered%20optical%20fiber%20sensor.pdf |
| _version_ | 1848857381659738112 |
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| author | Zainuddin, Nurul Hida Chee, Hui Yee Ahmad, Muhammad Zamharir Mahdi, Mohd Adzir Abu Bakar, Muhammad Hafiz Yaacob, Mohd Hanif |
| author_facet | Zainuddin, Nurul Hida Chee, Hui Yee Ahmad, Muhammad Zamharir Mahdi, Mohd Adzir Abu Bakar, Muhammad Hafiz Yaacob, Mohd Hanif |
| author_sort | Zainuddin, Nurul Hida |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This paper presents the development of tapered optical fiber sensor to detect a specific Leptospira bacteria DNA. The bacteria causes Leptospirosis, a deadly disease but with common early flu-like symptoms. Optical single mode fiber (SMF) of 125 μm diameter is tapered to produce 12 μm waist diameter and 15 cm length. The novel DNA-based optical fiber sensor is functionalized by incubating the tapered region with sodium hydroxide (NaOH), (3-Aminopropyl) triethoxysilane and glutaraldehyde. Probe DNA is immobilized onto the tapered region and subsequently hybridized by its complementary DNA (cDNA). The transmission spectra of the DNA-based optical fiber sensor are measured in the 1500 to 1600 nm wavelength range. It is discovered that the shift of the wavelength in the SMF sensor is linearly proportional with the increase in the cDNA concentrations from 0.1 to 1.0 nM. The sensitivity of the sensor toward DNA is measured to be 1.2862 nm/nM and able to detect as low as 0.1 fM. The sensor indicates high specificity when only minimal shift is detected for non-cDNA testing. The developed sensor is able to distinguish between actual DNA of Leptospira serovars (Canicola and Copenhageni) against Clostridium difficile (control sample) at very low (femtomolar) target concentrations. |
| first_indexed | 2025-11-15T11:56:39Z |
| format | Article |
| id | upm-73862 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:56:39Z |
| publishDate | 2018 |
| publisher | Wiley-VCH Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-738622020-05-20T15:34:44Z http://psasir.upm.edu.my/id/eprint/73862/ Sensitive leptospira DNA detection using tapered optical fiber sensor Zainuddin, Nurul Hida Chee, Hui Yee Ahmad, Muhammad Zamharir Mahdi, Mohd Adzir Abu Bakar, Muhammad Hafiz Yaacob, Mohd Hanif This paper presents the development of tapered optical fiber sensor to detect a specific Leptospira bacteria DNA. The bacteria causes Leptospirosis, a deadly disease but with common early flu-like symptoms. Optical single mode fiber (SMF) of 125 μm diameter is tapered to produce 12 μm waist diameter and 15 cm length. The novel DNA-based optical fiber sensor is functionalized by incubating the tapered region with sodium hydroxide (NaOH), (3-Aminopropyl) triethoxysilane and glutaraldehyde. Probe DNA is immobilized onto the tapered region and subsequently hybridized by its complementary DNA (cDNA). The transmission spectra of the DNA-based optical fiber sensor are measured in the 1500 to 1600 nm wavelength range. It is discovered that the shift of the wavelength in the SMF sensor is linearly proportional with the increase in the cDNA concentrations from 0.1 to 1.0 nM. The sensitivity of the sensor toward DNA is measured to be 1.2862 nm/nM and able to detect as low as 0.1 fM. The sensor indicates high specificity when only minimal shift is detected for non-cDNA testing. The developed sensor is able to distinguish between actual DNA of Leptospira serovars (Canicola and Copenhageni) against Clostridium difficile (control sample) at very low (femtomolar) target concentrations. Wiley-VCH Verlag 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73862/1/Sensitive%20leptospira%20DNA%20detection%20using%20tapered%20optical%20fiber%20sensor.pdf Zainuddin, Nurul Hida and Chee, Hui Yee and Ahmad, Muhammad Zamharir and Mahdi, Mohd Adzir and Abu Bakar, Muhammad Hafiz and Yaacob, Mohd Hanif (2018) Sensitive leptospira DNA detection using tapered optical fiber sensor. Journal of Biophotonics, 11 (8). art. no. 201700363. pp. 1-12. ISSN 1864-063X; ESSN: 1864-0648 https://onlinelibrary.wiley.com/doi/abs/10.1002/jbio.201700363 10.1002/jbio.201700363 |
| spellingShingle | Zainuddin, Nurul Hida Chee, Hui Yee Ahmad, Muhammad Zamharir Mahdi, Mohd Adzir Abu Bakar, Muhammad Hafiz Yaacob, Mohd Hanif Sensitive leptospira DNA detection using tapered optical fiber sensor |
| title | Sensitive leptospira DNA detection using tapered optical fiber sensor |
| title_full | Sensitive leptospira DNA detection using tapered optical fiber sensor |
| title_fullStr | Sensitive leptospira DNA detection using tapered optical fiber sensor |
| title_full_unstemmed | Sensitive leptospira DNA detection using tapered optical fiber sensor |
| title_short | Sensitive leptospira DNA detection using tapered optical fiber sensor |
| title_sort | sensitive leptospira dna detection using tapered optical fiber sensor |
| url | http://psasir.upm.edu.my/id/eprint/73862/ http://psasir.upm.edu.my/id/eprint/73862/ http://psasir.upm.edu.my/id/eprint/73862/ http://psasir.upm.edu.my/id/eprint/73862/1/Sensitive%20leptospira%20DNA%20detection%20using%20tapered%20optical%20fiber%20sensor.pdf |