Scoring system for lesions induced by different strains of Newcastle disease virus in chicken
Newcastle disease virus strains are velogenic, mesogenic, and lentogenic. This study aims to design a scoring system for lesions induced by different strains of Newcastle disease virus in chicken. Three experiments were conducted. In experiments 1 and 2, chickens were divided into infected and contr...
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| Format: | Article |
| Language: | English |
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Hindawi
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/73834/ http://psasir.upm.edu.my/id/eprint/73834/1/LESION.pdf |
| _version_ | 1848857373388570624 |
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| author | Hussein, Elawad A. Bejo, Mohd Hair Adamu, Lawan Omar, Abdul Rahman Arshad, Siti Suri Awad, Elmutaz Atta Ideris, Aini |
| author_facet | Hussein, Elawad A. Bejo, Mohd Hair Adamu, Lawan Omar, Abdul Rahman Arshad, Siti Suri Awad, Elmutaz Atta Ideris, Aini |
| author_sort | Hussein, Elawad A. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Newcastle disease virus strains are velogenic, mesogenic, and lentogenic. This study aims to design a scoring system for lesions induced by different strains of Newcastle disease virus in chicken. Three experiments were conducted. In experiments 1 and 2, chickens were divided into infected and control groups. Infected groups of experiments 1 and 2 consisted of 6 and 24 specific pathogen-free (SPF) chickens, respectively. Control groups in experiments 1 and 2 consisted of 6 and 15 SPF chickens, respectively. In infected groups, infection was induced by intranasal administration of 105 50% EID50/0.1 mL of velogenic Newcastle disease virus strain (vNDV). Infected chickens in experiment 1 were euthanised by cervical dislocation on days 3, 6, and 7 postinoculation (pi). Infected chickens in experiment 2 were euthanised at hours (hrs) 2, 4, 6, 12 and days 1, 2, 4, and 6 pi. Chickens of the control group in experiment 1 were euthanised on days 3 and 7 pi, whereas control group chickens in experiment 2 were euthanised on days 0, 1, 2, 4, and 6 pi. Then in experiment 3, 15 SPF chickens were divided into three groups; in the first group, 5 SPF chickens were infected with vNDV, in the second group, 5 SPF chickens were infected with lentogenic NDV (lNDV) (103.0 EID50/0.1 mL), and the third group was kept without infection as a control group. Chickens were euthanised on day 5 pi. In all previous experiments, tissues of brain, trachea, lung, caecal tonsil, liver, kidney, spleen, heart, proventriculus, intestine, and thymus were collected, fixed in 10% buffered formalin, embedded in paraffin, and sectioned. HS staining was applied. Tissues were examined under light microscope and changes were recorded. A scoring system was designed for lesions induced by different strains of NDV and, accordingly, lesions were scored. The scoring system was found helpful in the evaluation of disease severity. |
| first_indexed | 2025-11-15T11:56:31Z |
| format | Article |
| id | upm-73834 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:56:31Z |
| publishDate | 2018 |
| publisher | Hindawi |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-738342021-07-15T07:51:32Z http://psasir.upm.edu.my/id/eprint/73834/ Scoring system for lesions induced by different strains of Newcastle disease virus in chicken Hussein, Elawad A. Bejo, Mohd Hair Adamu, Lawan Omar, Abdul Rahman Arshad, Siti Suri Awad, Elmutaz Atta Ideris, Aini Newcastle disease virus strains are velogenic, mesogenic, and lentogenic. This study aims to design a scoring system for lesions induced by different strains of Newcastle disease virus in chicken. Three experiments were conducted. In experiments 1 and 2, chickens were divided into infected and control groups. Infected groups of experiments 1 and 2 consisted of 6 and 24 specific pathogen-free (SPF) chickens, respectively. Control groups in experiments 1 and 2 consisted of 6 and 15 SPF chickens, respectively. In infected groups, infection was induced by intranasal administration of 105 50% EID50/0.1 mL of velogenic Newcastle disease virus strain (vNDV). Infected chickens in experiment 1 were euthanised by cervical dislocation on days 3, 6, and 7 postinoculation (pi). Infected chickens in experiment 2 were euthanised at hours (hrs) 2, 4, 6, 12 and days 1, 2, 4, and 6 pi. Chickens of the control group in experiment 1 were euthanised on days 3 and 7 pi, whereas control group chickens in experiment 2 were euthanised on days 0, 1, 2, 4, and 6 pi. Then in experiment 3, 15 SPF chickens were divided into three groups; in the first group, 5 SPF chickens were infected with vNDV, in the second group, 5 SPF chickens were infected with lentogenic NDV (lNDV) (103.0 EID50/0.1 mL), and the third group was kept without infection as a control group. Chickens were euthanised on day 5 pi. In all previous experiments, tissues of brain, trachea, lung, caecal tonsil, liver, kidney, spleen, heart, proventriculus, intestine, and thymus were collected, fixed in 10% buffered formalin, embedded in paraffin, and sectioned. HS staining was applied. Tissues were examined under light microscope and changes were recorded. A scoring system was designed for lesions induced by different strains of NDV and, accordingly, lesions were scored. The scoring system was found helpful in the evaluation of disease severity. Hindawi 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73834/1/LESION.pdf Hussein, Elawad A. and Bejo, Mohd Hair and Adamu, Lawan and Omar, Abdul Rahman and Arshad, Siti Suri and Awad, Elmutaz Atta and Ideris, Aini (2018) Scoring system for lesions induced by different strains of Newcastle disease virus in chicken. Veterinary Medicine International, 2018. art. no. 9296520. pp. 1-9. ISSN 2090-8113; ESSN: 2042-0048 https://www.hindawi.com/journals/vmi/2018/9296520/ 10.1155/2018/9296520 |
| spellingShingle | Hussein, Elawad A. Bejo, Mohd Hair Adamu, Lawan Omar, Abdul Rahman Arshad, Siti Suri Awad, Elmutaz Atta Ideris, Aini Scoring system for lesions induced by different strains of Newcastle disease virus in chicken |
| title | Scoring system for lesions induced by different strains of Newcastle disease virus in chicken |
| title_full | Scoring system for lesions induced by different strains of Newcastle disease virus in chicken |
| title_fullStr | Scoring system for lesions induced by different strains of Newcastle disease virus in chicken |
| title_full_unstemmed | Scoring system for lesions induced by different strains of Newcastle disease virus in chicken |
| title_short | Scoring system for lesions induced by different strains of Newcastle disease virus in chicken |
| title_sort | scoring system for lesions induced by different strains of newcastle disease virus in chicken |
| url | http://psasir.upm.edu.my/id/eprint/73834/ http://psasir.upm.edu.my/id/eprint/73834/ http://psasir.upm.edu.my/id/eprint/73834/ http://psasir.upm.edu.my/id/eprint/73834/1/LESION.pdf |