Regulation of thiamine biosynthesis upon exogenous application of the vitamin in oil palm (Elaies guineensis)
Thiamine (vitamin B1) is an essential microelement that is synthesised de novo by plants and microorganisms. The active form of thiamine is thiamine pyrophosphate (TPP), which plays a prominent role in plant's metabolic activity particularly as an enzymatic cofactor. In vivo analysis of thiamin...
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| Format: | Article |
| Language: | English |
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Malaysian Palm Oil Board
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/73736/ http://psasir.upm.edu.my/id/eprint/73736/1/Regulation%20of%20thiamine%20biosynthesis%20upon%20exogenous%20application%20of%20the%20vitamin%20in%20oil%20palm.pdf |
| _version_ | 1848857350263275520 |
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| author | Subki, Atiqah Khairi Thaw, Siti Fariedda Balia Yusof, Zetty Norhana |
| author_facet | Subki, Atiqah Khairi Thaw, Siti Fariedda Balia Yusof, Zetty Norhana |
| author_sort | Subki, Atiqah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Thiamine (vitamin B1) is an essential microelement that is synthesised de novo by plants and microorganisms. The active form of thiamine is thiamine pyrophosphate (TPP), which plays a prominent role in plant's metabolic activity particularly as an enzymatic cofactor. In vivo analysis of thiamine in oil palm was performed where four months old oil palm seedlings were treated with 125 ml of 50 mM thiamine hydrochloride and tissue samples were collected at Day 0, 1, 2 and 3. The expression of ThiC gene fragment was analysed via quantitative real time polymerase chain reaction (qPCR) which showed an approximately five-fold decrease post-thiamine treatment. Analysis of thiamine and its derivatives via high performance liquid chromatography (HPLC) showed that the concentration of thiamine decreased, while TPP increased post-thiamine treatment. It is suggested that thiamine biosynthesis in oil palm can be effectively regulated at the physiological concentrations of the vitamin. |
| first_indexed | 2025-11-15T11:56:09Z |
| format | Article |
| id | upm-73736 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:56:09Z |
| publishDate | 2018 |
| publisher | Malaysian Palm Oil Board |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-737362021-07-15T23:58:24Z http://psasir.upm.edu.my/id/eprint/73736/ Regulation of thiamine biosynthesis upon exogenous application of the vitamin in oil palm (Elaies guineensis) Subki, Atiqah Khairi Thaw, Siti Fariedda Balia Yusof, Zetty Norhana Thiamine (vitamin B1) is an essential microelement that is synthesised de novo by plants and microorganisms. The active form of thiamine is thiamine pyrophosphate (TPP), which plays a prominent role in plant's metabolic activity particularly as an enzymatic cofactor. In vivo analysis of thiamine in oil palm was performed where four months old oil palm seedlings were treated with 125 ml of 50 mM thiamine hydrochloride and tissue samples were collected at Day 0, 1, 2 and 3. The expression of ThiC gene fragment was analysed via quantitative real time polymerase chain reaction (qPCR) which showed an approximately five-fold decrease post-thiamine treatment. Analysis of thiamine and its derivatives via high performance liquid chromatography (HPLC) showed that the concentration of thiamine decreased, while TPP increased post-thiamine treatment. It is suggested that thiamine biosynthesis in oil palm can be effectively regulated at the physiological concentrations of the vitamin. Malaysian Palm Oil Board 2018-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73736/1/Regulation%20of%20thiamine%20biosynthesis%20upon%20exogenous%20application%20of%20the%20vitamin%20in%20oil%20palm.pdf Subki, Atiqah and Khairi Thaw, Siti Fariedda and Balia Yusof, Zetty Norhana (2018) Regulation of thiamine biosynthesis upon exogenous application of the vitamin in oil palm (Elaies guineensis). Journal of Oil Palm Research, 30 (2). 236 - 241. ISSN 1511-2780 http://jopr.mpob.gov.my/regulation-of-thiamine-biosynthesis-upon-exogenous-application-of-the-vitamin-in-oil-palm-elaeis-guineensis/ 10.21894/jopr.2018.0028 |
| spellingShingle | Subki, Atiqah Khairi Thaw, Siti Fariedda Balia Yusof, Zetty Norhana Regulation of thiamine biosynthesis upon exogenous application of the vitamin in oil palm (Elaies guineensis) |
| title | Regulation of thiamine biosynthesis upon exogenous application of the vitamin in oil palm (Elaies guineensis) |
| title_full | Regulation of thiamine biosynthesis upon exogenous application of the vitamin in oil palm (Elaies guineensis) |
| title_fullStr | Regulation of thiamine biosynthesis upon exogenous application of the vitamin in oil palm (Elaies guineensis) |
| title_full_unstemmed | Regulation of thiamine biosynthesis upon exogenous application of the vitamin in oil palm (Elaies guineensis) |
| title_short | Regulation of thiamine biosynthesis upon exogenous application of the vitamin in oil palm (Elaies guineensis) |
| title_sort | regulation of thiamine biosynthesis upon exogenous application of the vitamin in oil palm (elaies guineensis) |
| url | http://psasir.upm.edu.my/id/eprint/73736/ http://psasir.upm.edu.my/id/eprint/73736/ http://psasir.upm.edu.my/id/eprint/73736/ http://psasir.upm.edu.my/id/eprint/73736/1/Regulation%20of%20thiamine%20biosynthesis%20upon%20exogenous%20application%20of%20the%20vitamin%20in%20oil%20palm.pdf |