Probiotic properties of exopolysaccharides-producing Lactobacillus isolated from tempoyak
Tempoyak is a functional Malaysian food (an acid-fermented condiment) which is produced from the pulp of the durian (Durio zibethinus) fruit. The current study aimed to isolate and identify potential exopolysaccharide (EPS)-producing Lactobacillus strains from tempoyak for potential use as probiotic...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/73644/ http://psasir.upm.edu.my/id/eprint/73644/1/Probiotic%20properties%20of%20exopolysaccharides-producing%20Lactobacillus%20isolated%20from%20tempoyak.pdf |
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| author | Suliman, Eilaf Suliman Khalil Abd Manap, Mohd Yazid Mustafa, Shuhaimi Alhelli, Amaal Mohammed Shokryazdan, Parisa |
| author_facet | Suliman, Eilaf Suliman Khalil Abd Manap, Mohd Yazid Mustafa, Shuhaimi Alhelli, Amaal Mohammed Shokryazdan, Parisa |
| author_sort | Suliman, Eilaf Suliman Khalil |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Tempoyak is a functional Malaysian food (an acid-fermented condiment) which is produced from the pulp of the durian (Durio zibethinus) fruit. The current study aimed to isolate and identify potential exopolysaccharide (EPS)-producing Lactobacillus strains from tempoyak for potential use as probiotics. Seven isolates (DUR2, DUR4, DUR5, DUR8, DUR12, DUR18, and DUR20) out of 44 were able to produce EPS, and exhibited resistance to acid and bile salt compared to the reference strains Lactobacillus rhmnosus (ATCC53103) and L. plantarum (ATCC8014). The seven isolated strains belonged to five different species—L. plantarum, L. fermentum, L. crispatus, L. reuteri, and L. pentosus—which were identified using API 50 CHL and 16S rRNA gene sequences (Polymerase chain reaction, PCR – based). The seven strains displayed different ability to produce EPS (100–850 mg/L). Isolates exhibited a high survivability to acid (pH 3.0), bile salts (0.3%), and gastrointestinal tract model (<70%). Results showed that the auto-aggregation and cell surface hydrophobicity ranged from 39.98% to 60.09% and 50.80% to 80.53%, respectively, whereas, the highest co-aggregation value (66.44%) was observed by L. fermentum (DUR8) with Pseudomonas aeruginosa. The isolates showed good inhibitory activity against tested pathogens, high antioxidant activity (32.29% to 73.36%), and good ability to reduce cholesterol (22.55% to 75.15%). Thus, the seven tested strains have value as probiotics. |
| first_indexed | 2025-11-15T11:55:45Z |
| format | Article |
| id | upm-73644 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:55:45Z |
| publishDate | 2018 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-736442020-05-10T15:55:03Z http://psasir.upm.edu.my/id/eprint/73644/ Probiotic properties of exopolysaccharides-producing Lactobacillus isolated from tempoyak Suliman, Eilaf Suliman Khalil Abd Manap, Mohd Yazid Mustafa, Shuhaimi Alhelli, Amaal Mohammed Shokryazdan, Parisa Tempoyak is a functional Malaysian food (an acid-fermented condiment) which is produced from the pulp of the durian (Durio zibethinus) fruit. The current study aimed to isolate and identify potential exopolysaccharide (EPS)-producing Lactobacillus strains from tempoyak for potential use as probiotics. Seven isolates (DUR2, DUR4, DUR5, DUR8, DUR12, DUR18, and DUR20) out of 44 were able to produce EPS, and exhibited resistance to acid and bile salt compared to the reference strains Lactobacillus rhmnosus (ATCC53103) and L. plantarum (ATCC8014). The seven isolated strains belonged to five different species—L. plantarum, L. fermentum, L. crispatus, L. reuteri, and L. pentosus—which were identified using API 50 CHL and 16S rRNA gene sequences (Polymerase chain reaction, PCR – based). The seven strains displayed different ability to produce EPS (100–850 mg/L). Isolates exhibited a high survivability to acid (pH 3.0), bile salts (0.3%), and gastrointestinal tract model (<70%). Results showed that the auto-aggregation and cell surface hydrophobicity ranged from 39.98% to 60.09% and 50.80% to 80.53%, respectively, whereas, the highest co-aggregation value (66.44%) was observed by L. fermentum (DUR8) with Pseudomonas aeruginosa. The isolates showed good inhibitory activity against tested pathogens, high antioxidant activity (32.29% to 73.36%), and good ability to reduce cholesterol (22.55% to 75.15%). Thus, the seven tested strains have value as probiotics. MDPI 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73644/1/Probiotic%20properties%20of%20exopolysaccharides-producing%20Lactobacillus%20isolated%20from%20tempoyak.pdf Suliman, Eilaf Suliman Khalil and Abd Manap, Mohd Yazid and Mustafa, Shuhaimi and Alhelli, Amaal Mohammed and Shokryazdan, Parisa (2018) Probiotic properties of exopolysaccharides-producing Lactobacillus isolated from tempoyak. Molecules, 23 (2). art. no. 398. pp. 1-20. ISSN 1420-3049; ESSN: 1420-3049 https://www.mdpi.com/1420-3049/23/2/398/htm 10.3390/molecules23020398 |
| spellingShingle | Suliman, Eilaf Suliman Khalil Abd Manap, Mohd Yazid Mustafa, Shuhaimi Alhelli, Amaal Mohammed Shokryazdan, Parisa Probiotic properties of exopolysaccharides-producing Lactobacillus isolated from tempoyak |
| title | Probiotic properties of exopolysaccharides-producing Lactobacillus isolated from tempoyak |
| title_full | Probiotic properties of exopolysaccharides-producing Lactobacillus isolated from tempoyak |
| title_fullStr | Probiotic properties of exopolysaccharides-producing Lactobacillus isolated from tempoyak |
| title_full_unstemmed | Probiotic properties of exopolysaccharides-producing Lactobacillus isolated from tempoyak |
| title_short | Probiotic properties of exopolysaccharides-producing Lactobacillus isolated from tempoyak |
| title_sort | probiotic properties of exopolysaccharides-producing lactobacillus isolated from tempoyak |
| url | http://psasir.upm.edu.my/id/eprint/73644/ http://psasir.upm.edu.my/id/eprint/73644/ http://psasir.upm.edu.my/id/eprint/73644/ http://psasir.upm.edu.my/id/eprint/73644/1/Probiotic%20properties%20of%20exopolysaccharides-producing%20Lactobacillus%20isolated%20from%20tempoyak.pdf |