Preliminary screening of plant proteases as a potential source for the development of an inhibitive assay for heavy metals
Heavy metals pollution has become a great threat to the world. Since instrumental methods are expensive and need skilled technician, a simple and fast method is needed to determine the presence of heavy metals in the environment. In this work, a preliminary study was carried out on the applicability...
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| Format: | Article |
| Language: | English |
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Hibiscus Publisher
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/73602/ http://psasir.upm.edu.my/id/eprint/73602/1/Preliminary%20screening%20of%20plant%20proteases%20as%20a%20potential%20source%20for%20the%20development%20of%20an%20inhibitive%20assay%20for%20heavy%20metals.pdf |
| _version_ | 1848857314617982976 |
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| author | Gunasekaran, Baskaran Wan Johari, Wan Lutfi Wasoh @ Mohamad Isa, Helmi Masdor, Noor Azlina Abd. Shukor, Mohd Yunus |
| author_facet | Gunasekaran, Baskaran Wan Johari, Wan Lutfi Wasoh @ Mohamad Isa, Helmi Masdor, Noor Azlina Abd. Shukor, Mohd Yunus |
| author_sort | Gunasekaran, Baskaran |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Heavy metals pollution has become a great threat to the world. Since instrumental methods are expensive and need skilled technician, a simple and fast method is needed to determine the presence of heavy metals in the environment. In this work, a preliminary study was carried out on the applicability of various local plants as a source of protease for the future development of the inhibitive enzyme assay for heavy-metals. The crude proteases preparation was assayed using casein as a substrate in conjunction with the Coomassie dye-binding assay. The crude protease from the kesinai plant was found to be the most potent plant protease. The crude enzyme exhibited broad temperature and pH ranges for activity and will be developed in the future as a potential inhibitive assay for heavy metals. |
| first_indexed | 2025-11-15T11:55:35Z |
| format | Article |
| id | upm-73602 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:55:35Z |
| publishDate | 2018 |
| publisher | Hibiscus Publisher |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-736022020-06-08T18:14:45Z http://psasir.upm.edu.my/id/eprint/73602/ Preliminary screening of plant proteases as a potential source for the development of an inhibitive assay for heavy metals Gunasekaran, Baskaran Wan Johari, Wan Lutfi Wasoh @ Mohamad Isa, Helmi Masdor, Noor Azlina Abd. Shukor, Mohd Yunus Heavy metals pollution has become a great threat to the world. Since instrumental methods are expensive and need skilled technician, a simple and fast method is needed to determine the presence of heavy metals in the environment. In this work, a preliminary study was carried out on the applicability of various local plants as a source of protease for the future development of the inhibitive enzyme assay for heavy-metals. The crude proteases preparation was assayed using casein as a substrate in conjunction with the Coomassie dye-binding assay. The crude protease from the kesinai plant was found to be the most potent plant protease. The crude enzyme exhibited broad temperature and pH ranges for activity and will be developed in the future as a potential inhibitive assay for heavy metals. Hibiscus Publisher 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73602/1/Preliminary%20screening%20of%20plant%20proteases%20as%20a%20potential%20source%20for%20the%20development%20of%20an%20inhibitive%20assay%20for%20heavy%20metals.pdf Gunasekaran, Baskaran and Wan Johari, Wan Lutfi and Wasoh @ Mohamad Isa, Helmi and Masdor, Noor Azlina and Abd. Shukor, Mohd Yunus (2018) Preliminary screening of plant proteases as a potential source for the development of an inhibitive assay for heavy metals. Bioremediation Science and Technology Research, 6 (1). pp. 9-13. ISSN ESSN: 2289-5892 https://journal.hibiscuspublisher.com/index.php/BSTR/article/view/393 |
| spellingShingle | Gunasekaran, Baskaran Wan Johari, Wan Lutfi Wasoh @ Mohamad Isa, Helmi Masdor, Noor Azlina Abd. Shukor, Mohd Yunus Preliminary screening of plant proteases as a potential source for the development of an inhibitive assay for heavy metals |
| title | Preliminary screening of plant proteases as a potential source for the development of an inhibitive assay for heavy metals |
| title_full | Preliminary screening of plant proteases as a potential source for the development of an inhibitive assay for heavy metals |
| title_fullStr | Preliminary screening of plant proteases as a potential source for the development of an inhibitive assay for heavy metals |
| title_full_unstemmed | Preliminary screening of plant proteases as a potential source for the development of an inhibitive assay for heavy metals |
| title_short | Preliminary screening of plant proteases as a potential source for the development of an inhibitive assay for heavy metals |
| title_sort | preliminary screening of plant proteases as a potential source for the development of an inhibitive assay for heavy metals |
| url | http://psasir.upm.edu.my/id/eprint/73602/ http://psasir.upm.edu.my/id/eprint/73602/ http://psasir.upm.edu.my/id/eprint/73602/1/Preliminary%20screening%20of%20plant%20proteases%20as%20a%20potential%20source%20for%20the%20development%20of%20an%20inhibitive%20assay%20for%20heavy%20metals.pdf |