Polyphenol-rich ethyl acetate fraction of Molineria latifolia rhizome restores oxidant-antioxidant balance by possible engagement of KEAP1-NRF2 and PKC/NF-κB signalling pathways
The present study examined the potential of ethyl acetate fraction (EAF) isolated from Molineria latifolia rhizome to modulate the oxidant-antioxidant balance in high fat diet and nicotinamide/streptozotocin-induced diabetic rats. Administration with EAF ameliorated systemic antioxidant status- and...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/73552/ http://psasir.upm.edu.my/id/eprint/73552/1/Polyphenol-rich%20ethyl%20acetate%20fraction%20of%20Molineria%20latifolia%20rhizome%20restores%20oxidant-antioxidant.pdf |
| _version_ | 1848857300706525184 |
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| author | Ooi, Der Jiun Chan, Kim Wei Ismail, Norsharina Imam, Mustapha Umar Ismail, Maznah |
| author_facet | Ooi, Der Jiun Chan, Kim Wei Ismail, Norsharina Imam, Mustapha Umar Ismail, Maznah |
| author_sort | Ooi, Der Jiun |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The present study examined the potential of ethyl acetate fraction (EAF) isolated from Molineria latifolia rhizome to modulate the oxidant-antioxidant balance in high fat diet and nicotinamide/streptozotocin-induced diabetic rats. Administration with EAF ameliorated systemic antioxidant status- and oxidative stress-related parameters without affecting the renal and liver functions in the diabetic rats. Further analyses on adipose, muscle and liver tissues demonstrated differing ability to scavenge free radicals and protection against lipid peroxidation. Transcriptional changes proposed concerted modulation of both KEAP1-NRF2 and PKC/NF-κB signallings in tissue-specific manner. Qualitative profiling of compounds present in EAF was analysed by non-targeted HPLC-QTOF mass spectrometry. A total of 23 unique mass signals were detected in EAF. Putative identification revealed a mixture of naturally occurring polyphenols ranging from cinnamic acid-, benzoic acid- and flavonoid-derived groups. Overall, the study demonstrated potential application of EAF to reinstate diabetes-induced oxidant-antioxidant imbalance by potentially modulating the NRF2–NF-κB signalling axis. |
| first_indexed | 2025-11-15T11:55:22Z |
| format | Article |
| id | upm-73552 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:55:22Z |
| publishDate | 2018 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-735522020-06-03T06:43:04Z http://psasir.upm.edu.my/id/eprint/73552/ Polyphenol-rich ethyl acetate fraction of Molineria latifolia rhizome restores oxidant-antioxidant balance by possible engagement of KEAP1-NRF2 and PKC/NF-κB signalling pathways Ooi, Der Jiun Chan, Kim Wei Ismail, Norsharina Imam, Mustapha Umar Ismail, Maznah The present study examined the potential of ethyl acetate fraction (EAF) isolated from Molineria latifolia rhizome to modulate the oxidant-antioxidant balance in high fat diet and nicotinamide/streptozotocin-induced diabetic rats. Administration with EAF ameliorated systemic antioxidant status- and oxidative stress-related parameters without affecting the renal and liver functions in the diabetic rats. Further analyses on adipose, muscle and liver tissues demonstrated differing ability to scavenge free radicals and protection against lipid peroxidation. Transcriptional changes proposed concerted modulation of both KEAP1-NRF2 and PKC/NF-κB signallings in tissue-specific manner. Qualitative profiling of compounds present in EAF was analysed by non-targeted HPLC-QTOF mass spectrometry. A total of 23 unique mass signals were detected in EAF. Putative identification revealed a mixture of naturally occurring polyphenols ranging from cinnamic acid-, benzoic acid- and flavonoid-derived groups. Overall, the study demonstrated potential application of EAF to reinstate diabetes-induced oxidant-antioxidant imbalance by potentially modulating the NRF2–NF-κB signalling axis. Elsevier 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73552/1/Polyphenol-rich%20ethyl%20acetate%20fraction%20of%20Molineria%20latifolia%20rhizome%20restores%20oxidant-antioxidant.pdf Ooi, Der Jiun and Chan, Kim Wei and Ismail, Norsharina and Imam, Mustapha Umar and Ismail, Maznah (2018) Polyphenol-rich ethyl acetate fraction of Molineria latifolia rhizome restores oxidant-antioxidant balance by possible engagement of KEAP1-NRF2 and PKC/NF-κB signalling pathways. Journal of Functional Foods, 42. 111 - 121. ISSN 1756-4646; ESSN: 2214-9414 https://www.sciencedirect.com/science/article/pii/S1756464617307168 10.1016/j.jff.2017.11.044 |
| spellingShingle | Ooi, Der Jiun Chan, Kim Wei Ismail, Norsharina Imam, Mustapha Umar Ismail, Maznah Polyphenol-rich ethyl acetate fraction of Molineria latifolia rhizome restores oxidant-antioxidant balance by possible engagement of KEAP1-NRF2 and PKC/NF-κB signalling pathways |
| title | Polyphenol-rich ethyl acetate fraction of Molineria latifolia rhizome restores oxidant-antioxidant balance by possible engagement of KEAP1-NRF2 and PKC/NF-κB signalling pathways |
| title_full | Polyphenol-rich ethyl acetate fraction of Molineria latifolia rhizome restores oxidant-antioxidant balance by possible engagement of KEAP1-NRF2 and PKC/NF-κB signalling pathways |
| title_fullStr | Polyphenol-rich ethyl acetate fraction of Molineria latifolia rhizome restores oxidant-antioxidant balance by possible engagement of KEAP1-NRF2 and PKC/NF-κB signalling pathways |
| title_full_unstemmed | Polyphenol-rich ethyl acetate fraction of Molineria latifolia rhizome restores oxidant-antioxidant balance by possible engagement of KEAP1-NRF2 and PKC/NF-κB signalling pathways |
| title_short | Polyphenol-rich ethyl acetate fraction of Molineria latifolia rhizome restores oxidant-antioxidant balance by possible engagement of KEAP1-NRF2 and PKC/NF-κB signalling pathways |
| title_sort | polyphenol-rich ethyl acetate fraction of molineria latifolia rhizome restores oxidant-antioxidant balance by possible engagement of keap1-nrf2 and pkc/nf-κb signalling pathways |
| url | http://psasir.upm.edu.my/id/eprint/73552/ http://psasir.upm.edu.my/id/eprint/73552/ http://psasir.upm.edu.my/id/eprint/73552/ http://psasir.upm.edu.my/id/eprint/73552/1/Polyphenol-rich%20ethyl%20acetate%20fraction%20of%20Molineria%20latifolia%20rhizome%20restores%20oxidant-antioxidant.pdf |