Plasma and urine metabolite profiling reveals the protective effect of Clinacanthus nutans in an ovalbumin-induced anaphylaxis model: ¹H-NMR metabolomics approach
The present study sought to identify the key biomarkers and pathways involved in the induction of allergic sensitization to ovalbumin and to elucidate the potential anti-anaphylaxis property of Clinacanthus nutans (Burm. f.) Lindau water leaf extract, a Southeast Asia herb in an in vivo ovalbumin-in...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/73538/ http://psasir.upm.edu.my/id/eprint/73538/1/Plasma%20and%20urine%20metabolite%20profiling%20reveals%20the%20protective%20effect%20of%20Clinacanthus%20nutans%20in%20an%20ovalbumin-induced%20anaphylaxis%20model.pdf |
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| author | Khoo, Leng Wei Kow, Audrey Siew Foong Maulidiani, Maulidiani Lee, Ming Tatt Tan, Chin Ping Shaari, Khozirah Tham, Chau Ling Abas, Faridah |
| author_facet | Khoo, Leng Wei Kow, Audrey Siew Foong Maulidiani, Maulidiani Lee, Ming Tatt Tan, Chin Ping Shaari, Khozirah Tham, Chau Ling Abas, Faridah |
| author_sort | Khoo, Leng Wei |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The present study sought to identify the key biomarkers and pathways involved in the induction of allergic sensitization to ovalbumin and to elucidate the potential anti-anaphylaxis property of Clinacanthus nutans (Burm. f.) Lindau water leaf extract, a Southeast Asia herb in an in vivo ovalbumin-induced active systemic anaphylaxis model evaluated by 1H-NMR metabolomics. The results revealed that carbohydrate metabolism (glucose, myo-inositol, galactarate) and lipid metabolism (glycerol, choline, sn-glycero-3-phosphocholine) are the key requisites for the induction of anaphylaxis reaction. Sensitized rats treated with 2000 mg/kg bw C. nutans extract before ovalbumin challenge showed a positive correlation with the normal group and was negatively related to the induced group. Further 1H-NMR analysis in complement with Kyoto Encyclopedia of Genes and Genomes (KEGG) reveals the protective effect of C. nutans extract against ovalbumin-induced anaphylaxis through the down-regulation of lipid metabolism (choline, sn-glycero-3-phosphocholine), carbohydrate and signal transduction system (glucose, myo-inositol, galactarate) and up-regulation of citrate cycle intermediates (citrate, 2-oxoglutarate, succinate), propanoate metabolism (1,2-propanediol), amino acid metabolism (betaine, N,N-dimethylglycine, methylguanidine, valine) and nucleotide metabolism (malonate, allantoin). In summary, this study reports for the first time, C. nutans water extract is a potential anti-anaphylactic agent and 1H-NMR metabolomics is a great alternative analytical tool to explicate the mechanism of action of anaphylaxis. |
| first_indexed | 2025-11-15T11:55:18Z |
| format | Article |
| id | upm-73538 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:55:18Z |
| publishDate | 2018 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-735382020-06-08T18:59:33Z http://psasir.upm.edu.my/id/eprint/73538/ Plasma and urine metabolite profiling reveals the protective effect of Clinacanthus nutans in an ovalbumin-induced anaphylaxis model: ¹H-NMR metabolomics approach Khoo, Leng Wei Kow, Audrey Siew Foong Maulidiani, Maulidiani Lee, Ming Tatt Tan, Chin Ping Shaari, Khozirah Tham, Chau Ling Abas, Faridah The present study sought to identify the key biomarkers and pathways involved in the induction of allergic sensitization to ovalbumin and to elucidate the potential anti-anaphylaxis property of Clinacanthus nutans (Burm. f.) Lindau water leaf extract, a Southeast Asia herb in an in vivo ovalbumin-induced active systemic anaphylaxis model evaluated by 1H-NMR metabolomics. The results revealed that carbohydrate metabolism (glucose, myo-inositol, galactarate) and lipid metabolism (glycerol, choline, sn-glycero-3-phosphocholine) are the key requisites for the induction of anaphylaxis reaction. Sensitized rats treated with 2000 mg/kg bw C. nutans extract before ovalbumin challenge showed a positive correlation with the normal group and was negatively related to the induced group. Further 1H-NMR analysis in complement with Kyoto Encyclopedia of Genes and Genomes (KEGG) reveals the protective effect of C. nutans extract against ovalbumin-induced anaphylaxis through the down-regulation of lipid metabolism (choline, sn-glycero-3-phosphocholine), carbohydrate and signal transduction system (glucose, myo-inositol, galactarate) and up-regulation of citrate cycle intermediates (citrate, 2-oxoglutarate, succinate), propanoate metabolism (1,2-propanediol), amino acid metabolism (betaine, N,N-dimethylglycine, methylguanidine, valine) and nucleotide metabolism (malonate, allantoin). In summary, this study reports for the first time, C. nutans water extract is a potential anti-anaphylactic agent and 1H-NMR metabolomics is a great alternative analytical tool to explicate the mechanism of action of anaphylaxis. Elsevier 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73538/1/Plasma%20and%20urine%20metabolite%20profiling%20reveals%20the%20protective%20effect%20of%20Clinacanthus%20nutans%20in%20an%20ovalbumin-induced%20anaphylaxis%20model.pdf Khoo, Leng Wei and Kow, Audrey Siew Foong and Maulidiani, Maulidiani and Lee, Ming Tatt and Tan, Chin Ping and Shaari, Khozirah and Tham, Chau Ling and Abas, Faridah (2018) Plasma and urine metabolite profiling reveals the protective effect of Clinacanthus nutans in an ovalbumin-induced anaphylaxis model: ¹H-NMR metabolomics approach. Journal of Pharmaceutical and Biomedical Analysis, 158. 438 - 450. ISSN 0731-7085; ESSN: 1873-264X https://www.sciencedirect.com/science/article/abs/pii/S0731708518308987 10.1016/j.jpba.2018.06.038 |
| spellingShingle | Khoo, Leng Wei Kow, Audrey Siew Foong Maulidiani, Maulidiani Lee, Ming Tatt Tan, Chin Ping Shaari, Khozirah Tham, Chau Ling Abas, Faridah Plasma and urine metabolite profiling reveals the protective effect of Clinacanthus nutans in an ovalbumin-induced anaphylaxis model: ¹H-NMR metabolomics approach |
| title | Plasma and urine metabolite profiling reveals the protective effect of Clinacanthus nutans in an ovalbumin-induced anaphylaxis model: ¹H-NMR metabolomics approach |
| title_full | Plasma and urine metabolite profiling reveals the protective effect of Clinacanthus nutans in an ovalbumin-induced anaphylaxis model: ¹H-NMR metabolomics approach |
| title_fullStr | Plasma and urine metabolite profiling reveals the protective effect of Clinacanthus nutans in an ovalbumin-induced anaphylaxis model: ¹H-NMR metabolomics approach |
| title_full_unstemmed | Plasma and urine metabolite profiling reveals the protective effect of Clinacanthus nutans in an ovalbumin-induced anaphylaxis model: ¹H-NMR metabolomics approach |
| title_short | Plasma and urine metabolite profiling reveals the protective effect of Clinacanthus nutans in an ovalbumin-induced anaphylaxis model: ¹H-NMR metabolomics approach |
| title_sort | plasma and urine metabolite profiling reveals the protective effect of clinacanthus nutans in an ovalbumin-induced anaphylaxis model: ¹h-nmr metabolomics approach |
| url | http://psasir.upm.edu.my/id/eprint/73538/ http://psasir.upm.edu.my/id/eprint/73538/ http://psasir.upm.edu.my/id/eprint/73538/ http://psasir.upm.edu.my/id/eprint/73538/1/Plasma%20and%20urine%20metabolite%20profiling%20reveals%20the%20protective%20effect%20of%20Clinacanthus%20nutans%20in%20an%20ovalbumin-induced%20anaphylaxis%20model.pdf |