Phyto-oestrogens affect fertilisation and embryo development in vitro in sheep
Phyto-oestrogens such as isoflavones are natural compounds that can profoundly affect reproductive function. In the present study, we tested whether including isoflavone compounds (genistein, biochanin A, formononetin) in the maturation medium would affect the outcomes for ovine oocytes in vitro. Ea...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
CSIRO Publishing
2018
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/73525/ http://psasir.upm.edu.my/id/eprint/73525/1/Phyto-oestrogens%20affect%20fertilisation%20and%20embryo%20development%20in%20vitro%20in%20sheep.pdf |
| _version_ | 1848857293919092736 |
|---|---|
| author | Amir, Anna Aryani Kelly, Jennifer M. Kleemann, David O. Durmic, Zoey Blache, Dominique Martin, Graeme B. |
| author_facet | Amir, Anna Aryani Kelly, Jennifer M. Kleemann, David O. Durmic, Zoey Blache, Dominique Martin, Graeme B. |
| author_sort | Amir, Anna Aryani |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Phyto-oestrogens such as isoflavones are natural compounds that can profoundly affect reproductive function. In the present study, we tested whether including isoflavone compounds (genistein, biochanin A, formononetin) in the maturation medium would affect the outcomes for ovine oocytes in vitro. Each isoflavone compound was evaluated at five concentrations (0, 2.5, 5, 10, 25µgmL-1) and the entire protocol was repeated four times. Cumulus-oocyte complexes were randomly allocated to the treatments, then fertilised and cultured in vitro. Compared with control (0µgmL-1), the lower concentrations of isoflavone (2.5, 5 and 10µgmL-1) had no detectable effect on the rates of cleavage or embryo development, or on embryo total cell counts (TCC). However, the highest concentration (25µgmL-1) of all three isoflavones exerted a variety of effects (P<0.05): genistein decreased cleavage rate, blastocyst rate and blastocyst efficiency (blastocysts produced per 100 oocytes); biochanin A decreased cleavage rate and blastocyst efficiency; and formononetin decreased blastocyst rate and blastocyst efficiency. Biochanin A (25µgmL-1) reduced embryo TCC specifically at the hatched blastocyst stage (P<0.05). We conclude that the presence of isoflavones at 25µgmL-1 during IVM decreases the cleavage rate and inhibits blastocyst hatching. |
| first_indexed | 2025-11-15T11:55:15Z |
| format | Article |
| id | upm-73525 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:55:15Z |
| publishDate | 2018 |
| publisher | CSIRO Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-735252020-06-07T17:54:20Z http://psasir.upm.edu.my/id/eprint/73525/ Phyto-oestrogens affect fertilisation and embryo development in vitro in sheep Amir, Anna Aryani Kelly, Jennifer M. Kleemann, David O. Durmic, Zoey Blache, Dominique Martin, Graeme B. Phyto-oestrogens such as isoflavones are natural compounds that can profoundly affect reproductive function. In the present study, we tested whether including isoflavone compounds (genistein, biochanin A, formononetin) in the maturation medium would affect the outcomes for ovine oocytes in vitro. Each isoflavone compound was evaluated at five concentrations (0, 2.5, 5, 10, 25µgmL-1) and the entire protocol was repeated four times. Cumulus-oocyte complexes were randomly allocated to the treatments, then fertilised and cultured in vitro. Compared with control (0µgmL-1), the lower concentrations of isoflavone (2.5, 5 and 10µgmL-1) had no detectable effect on the rates of cleavage or embryo development, or on embryo total cell counts (TCC). However, the highest concentration (25µgmL-1) of all three isoflavones exerted a variety of effects (P<0.05): genistein decreased cleavage rate, blastocyst rate and blastocyst efficiency (blastocysts produced per 100 oocytes); biochanin A decreased cleavage rate and blastocyst efficiency; and formononetin decreased blastocyst rate and blastocyst efficiency. Biochanin A (25µgmL-1) reduced embryo TCC specifically at the hatched blastocyst stage (P<0.05). We conclude that the presence of isoflavones at 25µgmL-1 during IVM decreases the cleavage rate and inhibits blastocyst hatching. CSIRO Publishing 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73525/1/Phyto-oestrogens%20affect%20fertilisation%20and%20embryo%20development%20in%20vitro%20in%20sheep.pdf Amir, Anna Aryani and Kelly, Jennifer M. and Kleemann, David O. and Durmic, Zoey and Blache, Dominique and Martin, Graeme B. (2018) Phyto-oestrogens affect fertilisation and embryo development in vitro in sheep. Reproduction, Fertility and Development, 30 (8). 1109 - 1115. ISSN 1031-3613; ESSN: 1448-5990 https://www.publish.csiro.au/RD/RD16481 10.1071/RD16481 |
| spellingShingle | Amir, Anna Aryani Kelly, Jennifer M. Kleemann, David O. Durmic, Zoey Blache, Dominique Martin, Graeme B. Phyto-oestrogens affect fertilisation and embryo development in vitro in sheep |
| title | Phyto-oestrogens affect fertilisation and embryo development in vitro in sheep |
| title_full | Phyto-oestrogens affect fertilisation and embryo development in vitro in sheep |
| title_fullStr | Phyto-oestrogens affect fertilisation and embryo development in vitro in sheep |
| title_full_unstemmed | Phyto-oestrogens affect fertilisation and embryo development in vitro in sheep |
| title_short | Phyto-oestrogens affect fertilisation and embryo development in vitro in sheep |
| title_sort | phyto-oestrogens affect fertilisation and embryo development in vitro in sheep |
| url | http://psasir.upm.edu.my/id/eprint/73525/ http://psasir.upm.edu.my/id/eprint/73525/ http://psasir.upm.edu.my/id/eprint/73525/ http://psasir.upm.edu.my/id/eprint/73525/1/Phyto-oestrogens%20affect%20fertilisation%20and%20embryo%20development%20in%20vitro%20in%20sheep.pdf |