Classification of raw stingless bee honeys by bee species origins using the NMR- and LC-MS-based metabolomics approach
The official standard for quality control of honey is currently based on physicochemical properties. However, this method is time-consuming, cost intensive, and does not lead to information on the originality of honey. This study aims to classify raw stingless bee honeys by bee species origins as a...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI Multidisciplinary Digital Publishing Institute
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/73407/ http://psasir.upm.edu.my/id/eprint/73407/1/BEE.pdf |
| _version_ | 1848857270916481024 |
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| author | Ismail, Intan Safinar Razali, Muhammad Taufiq Atsifa Shaari, Khozirah Abas, Faridah Maulidiani, Maulidiani Zainal, Zaim Akmal Zamri, Zulkifli Mohd Idrus, Zainuri Khatib, Alfi Yee, Soon Ling Lim, Leong Rui |
| author_facet | Ismail, Intan Safinar Razali, Muhammad Taufiq Atsifa Shaari, Khozirah Abas, Faridah Maulidiani, Maulidiani Zainal, Zaim Akmal Zamri, Zulkifli Mohd Idrus, Zainuri Khatib, Alfi Yee, Soon Ling Lim, Leong Rui |
| author_sort | Ismail, Intan Safinar |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The official standard for quality control of honey is currently based on physicochemical properties. However, this method is time-consuming, cost intensive, and does not lead to information on the originality of honey. This study aims to classify raw stingless bee honeys by bee species origins as a potential classifier using the NMR-LCMS-based metabolomics approach. Raw stingless bee honeys were analysed and classified by bee species origins using proton nuclear magnetic resonance (¹H-NMR) spectroscopy and an ultra-high performance liquid chromatography-quadrupole time of flight mass spectrometry (UHPLC-QTOF MS) in combination with chemometrics tools. The honey samples were able to be classified into three different groups based on the bee species origins of Heterotrigona itama, Geniotrigona thoracica, and Tetrigona apicalis. d-Fructofuranose (H. itama honey), β-d-Glucose, d-Xylose, α-d-Glucose (G. thoracica honey), and l-Lactic acid, Acetic acid, l-Alanine (T. apicalis honey) ident d-Fructofuranose identified via ¹H-NMR data and the diagnostic ions of UHPLC-QTOF MS were characterized as the discriminant metabolites or putative chemical markers. It could be suggested that the quality of honey in terms of originality and purity can be rapidly determined using the classification technique by bee species origins via the ¹H-NMR- and UHPLC-QTOF MS-based metabolomics approach. |
| first_indexed | 2025-11-15T11:54:53Z |
| format | Article |
| id | upm-73407 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:54:53Z |
| publishDate | 2018 |
| publisher | MDPI Multidisciplinary Digital Publishing Institute |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-734072021-02-12T09:13:07Z http://psasir.upm.edu.my/id/eprint/73407/ Classification of raw stingless bee honeys by bee species origins using the NMR- and LC-MS-based metabolomics approach Ismail, Intan Safinar Razali, Muhammad Taufiq Atsifa Shaari, Khozirah Abas, Faridah Maulidiani, Maulidiani Zainal, Zaim Akmal Zamri, Zulkifli Mohd Idrus, Zainuri Khatib, Alfi Yee, Soon Ling Lim, Leong Rui The official standard for quality control of honey is currently based on physicochemical properties. However, this method is time-consuming, cost intensive, and does not lead to information on the originality of honey. This study aims to classify raw stingless bee honeys by bee species origins as a potential classifier using the NMR-LCMS-based metabolomics approach. Raw stingless bee honeys were analysed and classified by bee species origins using proton nuclear magnetic resonance (¹H-NMR) spectroscopy and an ultra-high performance liquid chromatography-quadrupole time of flight mass spectrometry (UHPLC-QTOF MS) in combination with chemometrics tools. The honey samples were able to be classified into three different groups based on the bee species origins of Heterotrigona itama, Geniotrigona thoracica, and Tetrigona apicalis. d-Fructofuranose (H. itama honey), β-d-Glucose, d-Xylose, α-d-Glucose (G. thoracica honey), and l-Lactic acid, Acetic acid, l-Alanine (T. apicalis honey) ident d-Fructofuranose identified via ¹H-NMR data and the diagnostic ions of UHPLC-QTOF MS were characterized as the discriminant metabolites or putative chemical markers. It could be suggested that the quality of honey in terms of originality and purity can be rapidly determined using the classification technique by bee species origins via the ¹H-NMR- and UHPLC-QTOF MS-based metabolomics approach. MDPI Multidisciplinary Digital Publishing Institute 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73407/1/BEE.pdf Ismail, Intan Safinar and Razali, Muhammad Taufiq Atsifa and Shaari, Khozirah and Abas, Faridah and Maulidiani, Maulidiani and Zainal, Zaim Akmal and Zamri, Zulkifli and Mohd Idrus, Zainuri and Khatib, Alfi and Yee, Soon Ling and Lim, Leong Rui (2018) Classification of raw stingless bee honeys by bee species origins using the NMR- and LC-MS-based metabolomics approach. Molecules, 23 (9). pp. 1-18. ISSN 1420-3049 https://pubmed.ncbi.nlm.nih.gov/30154302/ 10.3390/molecules23092160 |
| spellingShingle | Ismail, Intan Safinar Razali, Muhammad Taufiq Atsifa Shaari, Khozirah Abas, Faridah Maulidiani, Maulidiani Zainal, Zaim Akmal Zamri, Zulkifli Mohd Idrus, Zainuri Khatib, Alfi Yee, Soon Ling Lim, Leong Rui Classification of raw stingless bee honeys by bee species origins using the NMR- and LC-MS-based metabolomics approach |
| title | Classification of raw stingless bee honeys by bee species origins using the NMR- and LC-MS-based metabolomics approach |
| title_full | Classification of raw stingless bee honeys by bee species origins using the NMR- and LC-MS-based metabolomics approach |
| title_fullStr | Classification of raw stingless bee honeys by bee species origins using the NMR- and LC-MS-based metabolomics approach |
| title_full_unstemmed | Classification of raw stingless bee honeys by bee species origins using the NMR- and LC-MS-based metabolomics approach |
| title_short | Classification of raw stingless bee honeys by bee species origins using the NMR- and LC-MS-based metabolomics approach |
| title_sort | classification of raw stingless bee honeys by bee species origins using the nmr- and lc-ms-based metabolomics approach |
| url | http://psasir.upm.edu.my/id/eprint/73407/ http://psasir.upm.edu.my/id/eprint/73407/ http://psasir.upm.edu.my/id/eprint/73407/ http://psasir.upm.edu.my/id/eprint/73407/1/BEE.pdf |