Improvement of bioethanol production in Consolidated Bioprocessing (CBP) via consortium of Aspergillus niger B2484 and Trichoderma asperellum B1581

Consolidated bioprocessing (CBP) in bioethanol production involves the combination of four essential biological procedures in a single bioreactor, using a mixture of organisms with favourable cellulolytic ability without the addition of exogenous enzymes. However, the main disadvantage of this proce...

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Main Authors: Mohamed Ghazali, Mona Fatin Syazwanee, Mustafa, Muskhazli, Mohd Zainudin, Nur Ain Izzati, Abd Aziz, Nor Azwady
Format: Article
Language:English
Published: Universiti Putra Malaysia Press 2021
Online Access:http://psasir.upm.edu.my/id/eprint/73290/
http://psasir.upm.edu.my/id/eprint/73290/1/CBP.pdf
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author Mohamed Ghazali, Mona Fatin Syazwanee
Mustafa, Muskhazli
Mohd Zainudin, Nur Ain Izzati
Abd Aziz, Nor Azwady
author_facet Mohamed Ghazali, Mona Fatin Syazwanee
Mustafa, Muskhazli
Mohd Zainudin, Nur Ain Izzati
Abd Aziz, Nor Azwady
author_sort Mohamed Ghazali, Mona Fatin Syazwanee
building UPM Institutional Repository
collection Online Access
description Consolidated bioprocessing (CBP) in bioethanol production involves the combination of four essential biological procedures in a single bioreactor, using a mixture of organisms with favourable cellulolytic ability without the addition of exogenous enzymes. However, the main disadvantage of this process is the complexity to optimise all factors considering both enzymes and microbial activity at the same time. Hence, this study aimed to optimise suitable culture conditions for both organisms to work efficiently. Six single factors that are considered crucial for bioethanol production were tested in one-factor-at-a-time (OFAT) analysis and analysed using Response Surface Methodology (RSM) software for Aspergillus niger B2484 and Trichoderma asperellum B1581 strains. The formulation of a new consortia setting was developed based on the average of two settings generated from RSM testing several combinations of consortia concentrations (5:1, 2:4, 3:3, 4:2, and 1:5). The combination of 5:1 Aspergillus niger B2484 and Trichoderma asperellum B1581 produced the most ethanol with 1.03 g/L, more than A. niger B2484, alone with 0.34 g/L of ethanol, indicating the potential of the combination of A. niger B2484 and T. asperellum B1581 co-culture for bioethanol production in CBP.
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spelling upm-732902021-08-16T10:02:17Z http://psasir.upm.edu.my/id/eprint/73290/ Improvement of bioethanol production in Consolidated Bioprocessing (CBP) via consortium of Aspergillus niger B2484 and Trichoderma asperellum B1581 Mohamed Ghazali, Mona Fatin Syazwanee Mustafa, Muskhazli Mohd Zainudin, Nur Ain Izzati Abd Aziz, Nor Azwady Consolidated bioprocessing (CBP) in bioethanol production involves the combination of four essential biological procedures in a single bioreactor, using a mixture of organisms with favourable cellulolytic ability without the addition of exogenous enzymes. However, the main disadvantage of this process is the complexity to optimise all factors considering both enzymes and microbial activity at the same time. Hence, this study aimed to optimise suitable culture conditions for both organisms to work efficiently. Six single factors that are considered crucial for bioethanol production were tested in one-factor-at-a-time (OFAT) analysis and analysed using Response Surface Methodology (RSM) software for Aspergillus niger B2484 and Trichoderma asperellum B1581 strains. The formulation of a new consortia setting was developed based on the average of two settings generated from RSM testing several combinations of consortia concentrations (5:1, 2:4, 3:3, 4:2, and 1:5). The combination of 5:1 Aspergillus niger B2484 and Trichoderma asperellum B1581 produced the most ethanol with 1.03 g/L, more than A. niger B2484, alone with 0.34 g/L of ethanol, indicating the potential of the combination of A. niger B2484 and T. asperellum B1581 co-culture for bioethanol production in CBP. Universiti Putra Malaysia Press 2021-01 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73290/1/CBP.pdf Mohamed Ghazali, Mona Fatin Syazwanee and Mustafa, Muskhazli and Mohd Zainudin, Nur Ain Izzati and Abd Aziz, Nor Azwady (2021) Improvement of bioethanol production in Consolidated Bioprocessing (CBP) via consortium of Aspergillus niger B2484 and Trichoderma asperellum B1581. Pertanika Journal of Science & Technology, 29 (1). pp. 301-316. ISSN 0128-7680; ESSN: 2231-8526 http://www.pertanika.upm.edu.my/pjst/browse/regular-issue?article=JST-2201-2020 10.47836/pjst.29.1.17
spellingShingle Mohamed Ghazali, Mona Fatin Syazwanee
Mustafa, Muskhazli
Mohd Zainudin, Nur Ain Izzati
Abd Aziz, Nor Azwady
Improvement of bioethanol production in Consolidated Bioprocessing (CBP) via consortium of Aspergillus niger B2484 and Trichoderma asperellum B1581
title Improvement of bioethanol production in Consolidated Bioprocessing (CBP) via consortium of Aspergillus niger B2484 and Trichoderma asperellum B1581
title_full Improvement of bioethanol production in Consolidated Bioprocessing (CBP) via consortium of Aspergillus niger B2484 and Trichoderma asperellum B1581
title_fullStr Improvement of bioethanol production in Consolidated Bioprocessing (CBP) via consortium of Aspergillus niger B2484 and Trichoderma asperellum B1581
title_full_unstemmed Improvement of bioethanol production in Consolidated Bioprocessing (CBP) via consortium of Aspergillus niger B2484 and Trichoderma asperellum B1581
title_short Improvement of bioethanol production in Consolidated Bioprocessing (CBP) via consortium of Aspergillus niger B2484 and Trichoderma asperellum B1581
title_sort improvement of bioethanol production in consolidated bioprocessing (cbp) via consortium of aspergillus niger b2484 and trichoderma asperellum b1581
url http://psasir.upm.edu.my/id/eprint/73290/
http://psasir.upm.edu.my/id/eprint/73290/
http://psasir.upm.edu.my/id/eprint/73290/
http://psasir.upm.edu.my/id/eprint/73290/1/CBP.pdf