Autolysis of bovine skin, its endogenous proteases, protease inhibitors and their effects on quality characteristics of extracted gelatin
The autolysis of pretreated bovine skin (PBS) (treated with 0.1 M NaOH and 1% HCl), its endogenous proteases, inhibitors and their effects on quality attributes of gelatin were examined. PBS was subjected to different temperatures (20–90 °C) and pH (2–9) and treated with different protease inhibitor...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/73245/ http://psasir.upm.edu.my/id/eprint/73245/1/BOVINE.pdf |
| _version_ | 1848857252839030784 |
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| author | Sazili, Awis Qurni Dauda, Adeyemi Kazeem Ahmad, Tanbir Leo, Teik Kee Mohamed, Elmutaz Atta Awad Ahmad, Siti Aqlima Ismail, Amin Abdul Khalil, Khalilah |
| author_facet | Sazili, Awis Qurni Dauda, Adeyemi Kazeem Ahmad, Tanbir Leo, Teik Kee Mohamed, Elmutaz Atta Awad Ahmad, Siti Aqlima Ismail, Amin Abdul Khalil, Khalilah |
| author_sort | Sazili, Awis Qurni |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The autolysis of pretreated bovine skin (PBS) (treated with 0.1 M NaOH and 1% HCl), its endogenous proteases, inhibitors and their effects on quality attributes of gelatin were examined. PBS was subjected to different temperatures (20–90 °C) and pH (2–9) and treated with different protease inhibitors. Maximum autolytic activity of PBS was observed at 40 °C and pH 5. Ethylene-bis (oxyethylenenitrilo) tetraacetic acid (EGTA) was the most effective in impeding the degradation of γ-, β- and α- chains of PBS protein indicating that metallocollagenases were the predominant endogenous proteases in bovine skin. Gelatin was extracted in the absence (GAE) and presence (GPE) of EGTA, and EGTA with papain enzyme (GPEP). GPEP had a higher yield and lower gel strength than GEA and GPE. Metallocollagenases partook in the degradation of gelatin thereby affecting its functional properties. Pretreating PBS with or without EGTA, and papain influenced the quality attributes of gelatin. |
| first_indexed | 2025-11-15T11:54:36Z |
| format | Article |
| id | upm-73245 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:54:36Z |
| publishDate | 2018 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-732452020-11-30T07:59:33Z http://psasir.upm.edu.my/id/eprint/73245/ Autolysis of bovine skin, its endogenous proteases, protease inhibitors and their effects on quality characteristics of extracted gelatin Sazili, Awis Qurni Dauda, Adeyemi Kazeem Ahmad, Tanbir Leo, Teik Kee Mohamed, Elmutaz Atta Awad Ahmad, Siti Aqlima Ismail, Amin Abdul Khalil, Khalilah The autolysis of pretreated bovine skin (PBS) (treated with 0.1 M NaOH and 1% HCl), its endogenous proteases, inhibitors and their effects on quality attributes of gelatin were examined. PBS was subjected to different temperatures (20–90 °C) and pH (2–9) and treated with different protease inhibitors. Maximum autolytic activity of PBS was observed at 40 °C and pH 5. Ethylene-bis (oxyethylenenitrilo) tetraacetic acid (EGTA) was the most effective in impeding the degradation of γ-, β- and α- chains of PBS protein indicating that metallocollagenases were the predominant endogenous proteases in bovine skin. Gelatin was extracted in the absence (GAE) and presence (GPE) of EGTA, and EGTA with papain enzyme (GPEP). GPEP had a higher yield and lower gel strength than GEA and GPE. Metallocollagenases partook in the degradation of gelatin thereby affecting its functional properties. Pretreating PBS with or without EGTA, and papain influenced the quality attributes of gelatin. Elsevier 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73245/1/BOVINE.pdf Sazili, Awis Qurni and Dauda, Adeyemi Kazeem and Ahmad, Tanbir and Leo, Teik Kee and Mohamed, Elmutaz Atta Awad and Ahmad, Siti Aqlima and Ismail, Amin and Abdul Khalil, Khalilah (2018) Autolysis of bovine skin, its endogenous proteases, protease inhibitors and their effects on quality characteristics of extracted gelatin. Food Chemistry, 265. pp. 1-8. ISSN 1873-7072; ESSN: 0308-8146 https://www.sciencedirect.com/science/article/abs/pii/S0308814618308410#! 10.1016/j.foodchem.2018.05.046 |
| spellingShingle | Sazili, Awis Qurni Dauda, Adeyemi Kazeem Ahmad, Tanbir Leo, Teik Kee Mohamed, Elmutaz Atta Awad Ahmad, Siti Aqlima Ismail, Amin Abdul Khalil, Khalilah Autolysis of bovine skin, its endogenous proteases, protease inhibitors and their effects on quality characteristics of extracted gelatin |
| title | Autolysis of bovine skin, its endogenous proteases, protease inhibitors and their effects on quality characteristics of extracted gelatin |
| title_full | Autolysis of bovine skin, its endogenous proteases, protease inhibitors and their effects on quality characteristics of extracted gelatin |
| title_fullStr | Autolysis of bovine skin, its endogenous proteases, protease inhibitors and their effects on quality characteristics of extracted gelatin |
| title_full_unstemmed | Autolysis of bovine skin, its endogenous proteases, protease inhibitors and their effects on quality characteristics of extracted gelatin |
| title_short | Autolysis of bovine skin, its endogenous proteases, protease inhibitors and their effects on quality characteristics of extracted gelatin |
| title_sort | autolysis of bovine skin, its endogenous proteases, protease inhibitors and their effects on quality characteristics of extracted gelatin |
| url | http://psasir.upm.edu.my/id/eprint/73245/ http://psasir.upm.edu.my/id/eprint/73245/ http://psasir.upm.edu.my/id/eprint/73245/ http://psasir.upm.edu.my/id/eprint/73245/1/BOVINE.pdf |