Antioxidant and anticancer activities of enzymatic eel (monopterus sp) protein hydrolysate as influenced by different molecular weight
This study aims to investigate the antioxidant and anticancer properties of fractionated eel protein hydrolysate (EPH) as well as qualitatively determine its free fatty acids. The eel flesh was enzymatically hydrolyzed and fractionated through membrane filter (10 kDa, 5 kDa and 3 kDa). The lipid per...
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| Format: | Article |
| Language: | English |
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Elsevier
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/73166/ http://psasir.upm.edu.my/id/eprint/73166/1/EEL.pdf |
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| author | Azlan, Azrina Mhd Sarbon, Norizah Abdul Halim, Nura Ruhaya Mohd Yusof, Hayati |
| author_facet | Azlan, Azrina Mhd Sarbon, Norizah Abdul Halim, Nura Ruhaya Mohd Yusof, Hayati |
| author_sort | Azlan, Azrina |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study aims to investigate the antioxidant and anticancer properties of fractionated eel protein hydrolysate (EPH) as well as qualitatively determine its free fatty acids. The eel flesh was enzymatically hydrolyzed and fractionated through membrane filter (10 kDa, 5 kDa and 3 kDa). The lipid peroxidation assays and mechanisms of antioxidant activity (reducing power, ferrous ion chelating activity and 1, 1-diphenylpicrylhydrazyl (DPPH) radical scavenging activity) of fractionated EPH were determined. The anticancer activity was determined by 3–4, 5-dimethylthiazol-2-yl-2, 5-diphenyltetrazolium bromide (MTT) assay using MCF-7 cell lines. Free fatty acids in eel flesh and EPH were determined using gas chromatography. The results obtained showed that 3 kDa EPH possessed the highest inhibition of lipid peroxidation, reducing power, DPPH scavenging activity and anticancer activity. Moreover, the changes of unsaturated fatty acids during hydrolysis process resulting in more stable hydrolysate towards oxidation. Based on the mechanisms of antioxidant activity conducted, this study found that the EPH had more ability as primary antioxidant than secondary antioxidant. |
| first_indexed | 2025-11-15T11:54:29Z |
| format | Article |
| id | upm-73166 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:54:29Z |
| publishDate | 2018 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-731662020-11-26T22:59:39Z http://psasir.upm.edu.my/id/eprint/73166/ Antioxidant and anticancer activities of enzymatic eel (monopterus sp) protein hydrolysate as influenced by different molecular weight Azlan, Azrina Mhd Sarbon, Norizah Abdul Halim, Nura Ruhaya Mohd Yusof, Hayati This study aims to investigate the antioxidant and anticancer properties of fractionated eel protein hydrolysate (EPH) as well as qualitatively determine its free fatty acids. The eel flesh was enzymatically hydrolyzed and fractionated through membrane filter (10 kDa, 5 kDa and 3 kDa). The lipid peroxidation assays and mechanisms of antioxidant activity (reducing power, ferrous ion chelating activity and 1, 1-diphenylpicrylhydrazyl (DPPH) radical scavenging activity) of fractionated EPH were determined. The anticancer activity was determined by 3–4, 5-dimethylthiazol-2-yl-2, 5-diphenyltetrazolium bromide (MTT) assay using MCF-7 cell lines. Free fatty acids in eel flesh and EPH were determined using gas chromatography. The results obtained showed that 3 kDa EPH possessed the highest inhibition of lipid peroxidation, reducing power, DPPH scavenging activity and anticancer activity. Moreover, the changes of unsaturated fatty acids during hydrolysis process resulting in more stable hydrolysate towards oxidation. Based on the mechanisms of antioxidant activity conducted, this study found that the EPH had more ability as primary antioxidant than secondary antioxidant. Elsevier 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73166/1/EEL.pdf Azlan, Azrina and Mhd Sarbon, Norizah and Abdul Halim, Nura Ruhaya and Mohd Yusof, Hayati (2018) Antioxidant and anticancer activities of enzymatic eel (monopterus sp) protein hydrolysate as influenced by different molecular weight. Biocatalysis and Agricultural Biotechnology, 16. pp. 10-16. ISSN 1878-8181 https://www.sciencedirect.com/science/article/abs/pii/S1878818118301804 10.1016/j.bcab.2018.06.006 |
| spellingShingle | Azlan, Azrina Mhd Sarbon, Norizah Abdul Halim, Nura Ruhaya Mohd Yusof, Hayati Antioxidant and anticancer activities of enzymatic eel (monopterus sp) protein hydrolysate as influenced by different molecular weight |
| title | Antioxidant and anticancer activities of enzymatic eel (monopterus sp) protein hydrolysate as influenced by different molecular weight |
| title_full | Antioxidant and anticancer activities of enzymatic eel (monopterus sp) protein hydrolysate as influenced by different molecular weight |
| title_fullStr | Antioxidant and anticancer activities of enzymatic eel (monopterus sp) protein hydrolysate as influenced by different molecular weight |
| title_full_unstemmed | Antioxidant and anticancer activities of enzymatic eel (monopterus sp) protein hydrolysate as influenced by different molecular weight |
| title_short | Antioxidant and anticancer activities of enzymatic eel (monopterus sp) protein hydrolysate as influenced by different molecular weight |
| title_sort | antioxidant and anticancer activities of enzymatic eel (monopterus sp) protein hydrolysate as influenced by different molecular weight |
| url | http://psasir.upm.edu.my/id/eprint/73166/ http://psasir.upm.edu.my/id/eprint/73166/ http://psasir.upm.edu.my/id/eprint/73166/ http://psasir.upm.edu.my/id/eprint/73166/1/EEL.pdf |