Anticancer potential of Alternanthera sessilis extract on HT-29 human colon cancer cells
Objective: To identify the bioactive extracts from Alternanthera sessilis and investigate its cytotoxicity potential against colon cancer cells, HT-29. Methods: This study examined the effects of three parts (aerial, leaf, stem) of whole plant on HT-29 colon cancer cell lines. Three different extrac...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/73151/ http://psasir.upm.edu.my/id/eprint/73151/1/CANCER.pdf |
| _version_ | 1848857242521042944 |
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| author | Mohd Esa, Norhaizan Muniandy, Katyakyini Sivapragasam, Gothai Subbiah, Suresh Kumar Arulselvan, Palanisamy |
| author_facet | Mohd Esa, Norhaizan Muniandy, Katyakyini Sivapragasam, Gothai Subbiah, Suresh Kumar Arulselvan, Palanisamy |
| author_sort | Mohd Esa, Norhaizan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Objective: To identify the bioactive extracts from Alternanthera sessilis and investigate its cytotoxicity potential against colon cancer cells, HT-29. Methods: This study examined the effects of three parts (aerial, leaf, stem) of whole plant on HT-29 colon cancer cell lines. Three different extracts from the plant parts were prepared by maceration technique using 80% ethanol. The anticancer activities were determined using MTT, clonogenic, cell motility and AOPI assay. The chemical composition profiling was analyzed by GC-MS. Results: Among three plant part extracts, leaf extract greatly suppressed the growth of colon cancer cells in time and dosage-dependent manner, followed by aerial and stem. The cytotoxicity results were rationalized with clonogenic, cell motility and AO/PI assay, where extract showed the most active activity compared to aerial and stem extracts. GC-MS analysis of leaf extract showed there were various recognized anti-cancer, anti-oxidant and anti-inflammatory compounds. Conclusions: Amid the screened extracts, the leaf extract exhibits the credible cytotoxic, anti-proliferative and apoptotic activity and hence, our findings call for additional research to conclude the active compounds and their mechanisms determining the apoptotic activity. |
| first_indexed | 2025-11-15T11:54:26Z |
| format | Article |
| id | upm-73151 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:54:26Z |
| publishDate | 2018 |
| publisher | Wolters Kluwer Medknow Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-731512021-01-26T19:58:10Z http://psasir.upm.edu.my/id/eprint/73151/ Anticancer potential of Alternanthera sessilis extract on HT-29 human colon cancer cells Mohd Esa, Norhaizan Muniandy, Katyakyini Sivapragasam, Gothai Subbiah, Suresh Kumar Arulselvan, Palanisamy Objective: To identify the bioactive extracts from Alternanthera sessilis and investigate its cytotoxicity potential against colon cancer cells, HT-29. Methods: This study examined the effects of three parts (aerial, leaf, stem) of whole plant on HT-29 colon cancer cell lines. Three different extracts from the plant parts were prepared by maceration technique using 80% ethanol. The anticancer activities were determined using MTT, clonogenic, cell motility and AOPI assay. The chemical composition profiling was analyzed by GC-MS. Results: Among three plant part extracts, leaf extract greatly suppressed the growth of colon cancer cells in time and dosage-dependent manner, followed by aerial and stem. The cytotoxicity results were rationalized with clonogenic, cell motility and AO/PI assay, where extract showed the most active activity compared to aerial and stem extracts. GC-MS analysis of leaf extract showed there were various recognized anti-cancer, anti-oxidant and anti-inflammatory compounds. Conclusions: Amid the screened extracts, the leaf extract exhibits the credible cytotoxic, anti-proliferative and apoptotic activity and hence, our findings call for additional research to conclude the active compounds and their mechanisms determining the apoptotic activity. Wolters Kluwer Medknow Publications 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73151/1/CANCER.pdf Mohd Esa, Norhaizan and Muniandy, Katyakyini and Sivapragasam, Gothai and Subbiah, Suresh Kumar and Arulselvan, Palanisamy (2018) Anticancer potential of Alternanthera sessilis extract on HT-29 human colon cancer cells. Asian Pacific Journal of Tropical Biomedicine, 8 (8). 394 - 402. ISSN 2221-1691; ESSN: 2588-9222 https://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=8;spage=394;epage=402;aulast=Gothai 10.4103/2221-1691.239427 |
| spellingShingle | Mohd Esa, Norhaizan Muniandy, Katyakyini Sivapragasam, Gothai Subbiah, Suresh Kumar Arulselvan, Palanisamy Anticancer potential of Alternanthera sessilis extract on HT-29 human colon cancer cells |
| title | Anticancer potential of Alternanthera sessilis extract on HT-29 human colon cancer cells |
| title_full | Anticancer potential of Alternanthera sessilis extract on HT-29 human colon cancer cells |
| title_fullStr | Anticancer potential of Alternanthera sessilis extract on HT-29 human colon cancer cells |
| title_full_unstemmed | Anticancer potential of Alternanthera sessilis extract on HT-29 human colon cancer cells |
| title_short | Anticancer potential of Alternanthera sessilis extract on HT-29 human colon cancer cells |
| title_sort | anticancer potential of alternanthera sessilis extract on ht-29 human colon cancer cells |
| url | http://psasir.upm.edu.my/id/eprint/73151/ http://psasir.upm.edu.my/id/eprint/73151/ http://psasir.upm.edu.my/id/eprint/73151/ http://psasir.upm.edu.my/id/eprint/73151/1/CANCER.pdf |