Gold nanoparticle-decorated reduced-graphene oxide targeting anti hepatitis B virus core antigen
Hepatitis B virus core antigen (HBcAg) is the major structural protein of hepatitis B virus (HBV). The presence of anti-HBcAg antibody in a blood serum indicates that a person has been exposed to HBV. This study demonstrated that the immobilization of HBcAg onto the gold nanoparticles-decorated redu...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/72850/ http://psasir.upm.edu.my/id/eprint/72850/1/Gold%20nanoparticle-decorated%20.pdf |
| _version_ | 1848857218097610752 |
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| author | Abd Muain, Mohamad Farid Cheo, Kooi Hoong Omar, Muhamad Nadzmi Amir Hamzah, Amir Syahir Lim, Hong Ngee Salleh, Abu Bakar Tan, Wen Siang Ahmad Tajudin, Asilah |
| author_facet | Abd Muain, Mohamad Farid Cheo, Kooi Hoong Omar, Muhamad Nadzmi Amir Hamzah, Amir Syahir Lim, Hong Ngee Salleh, Abu Bakar Tan, Wen Siang Ahmad Tajudin, Asilah |
| author_sort | Abd Muain, Mohamad Farid |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Hepatitis B virus core antigen (HBcAg) is the major structural protein of hepatitis B virus (HBV). The presence of anti-HBcAg antibody in a blood serum indicates that a person has been exposed to HBV. This study demonstrated that the immobilization of HBcAg onto the gold nanoparticles-decorated reduced graphene oxide (rGO-en-AuNPs) nanocomposite could be used as an antigen-functionalized surface to sense the presence of anti-HBcAg. The modified rGO-en-AuNPs/HBcAg was then allowed to undergo impedimetric detection of anti-HBcAg with anti-estradiol antibody and bovine serum albumin as the interferences. Upon successful detection of anti-HBcAg in spiked buffer samples, impedimetric detection of the antibody was then further carried out in spiked human serum samples. The electrochemical response showed a linear relationship between electron transfer resistance and the concentration of anti-HBcAg ranging from 3.91 ng mL−1 to 125.00 ng mL−1 with lowest limit of detection (LOD) of 3.80 ng mL−1 at 3 σ m−1. This established method exhibits potential as a fast and convenient way to detect anti-HBcAg. |
| first_indexed | 2025-11-15T11:54:03Z |
| format | Article |
| id | upm-72850 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:54:03Z |
| publishDate | 2018 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-728502021-03-17T00:54:45Z http://psasir.upm.edu.my/id/eprint/72850/ Gold nanoparticle-decorated reduced-graphene oxide targeting anti hepatitis B virus core antigen Abd Muain, Mohamad Farid Cheo, Kooi Hoong Omar, Muhamad Nadzmi Amir Hamzah, Amir Syahir Lim, Hong Ngee Salleh, Abu Bakar Tan, Wen Siang Ahmad Tajudin, Asilah Hepatitis B virus core antigen (HBcAg) is the major structural protein of hepatitis B virus (HBV). The presence of anti-HBcAg antibody in a blood serum indicates that a person has been exposed to HBV. This study demonstrated that the immobilization of HBcAg onto the gold nanoparticles-decorated reduced graphene oxide (rGO-en-AuNPs) nanocomposite could be used as an antigen-functionalized surface to sense the presence of anti-HBcAg. The modified rGO-en-AuNPs/HBcAg was then allowed to undergo impedimetric detection of anti-HBcAg with anti-estradiol antibody and bovine serum albumin as the interferences. Upon successful detection of anti-HBcAg in spiked buffer samples, impedimetric detection of the antibody was then further carried out in spiked human serum samples. The electrochemical response showed a linear relationship between electron transfer resistance and the concentration of anti-HBcAg ranging from 3.91 ng mL−1 to 125.00 ng mL−1 with lowest limit of detection (LOD) of 3.80 ng mL−1 at 3 σ m−1. This established method exhibits potential as a fast and convenient way to detect anti-HBcAg. Elsevier 2018-08 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72850/1/Gold%20nanoparticle-decorated%20.pdf Abd Muain, Mohamad Farid and Cheo, Kooi Hoong and Omar, Muhamad Nadzmi and Amir Hamzah, Amir Syahir and Lim, Hong Ngee and Salleh, Abu Bakar and Tan, Wen Siang and Ahmad Tajudin, Asilah (2018) Gold nanoparticle-decorated reduced-graphene oxide targeting anti hepatitis B virus core antigen. Bioelectrochemistry, 122. 199 - 205. ISSN 1567-5394; ESSN: 1878-562X https://www.sciencedirect.com/science/article/pii/S1567539418300422 10.1016/j.bioelechem.2018.04.004 |
| spellingShingle | Abd Muain, Mohamad Farid Cheo, Kooi Hoong Omar, Muhamad Nadzmi Amir Hamzah, Amir Syahir Lim, Hong Ngee Salleh, Abu Bakar Tan, Wen Siang Ahmad Tajudin, Asilah Gold nanoparticle-decorated reduced-graphene oxide targeting anti hepatitis B virus core antigen |
| title | Gold nanoparticle-decorated reduced-graphene oxide targeting anti hepatitis B virus core antigen |
| title_full | Gold nanoparticle-decorated reduced-graphene oxide targeting anti hepatitis B virus core antigen |
| title_fullStr | Gold nanoparticle-decorated reduced-graphene oxide targeting anti hepatitis B virus core antigen |
| title_full_unstemmed | Gold nanoparticle-decorated reduced-graphene oxide targeting anti hepatitis B virus core antigen |
| title_short | Gold nanoparticle-decorated reduced-graphene oxide targeting anti hepatitis B virus core antigen |
| title_sort | gold nanoparticle-decorated reduced-graphene oxide targeting anti hepatitis b virus core antigen |
| url | http://psasir.upm.edu.my/id/eprint/72850/ http://psasir.upm.edu.my/id/eprint/72850/ http://psasir.upm.edu.my/id/eprint/72850/ http://psasir.upm.edu.my/id/eprint/72850/1/Gold%20nanoparticle-decorated%20.pdf |