Biodiversity of chloramphenicol-resistant mesophilic heterotrophs from Southeast Asian aquaculture environments
In the present study, samples of pond water, sediment and farmed species were collected at 12 fish and shrimp farms in Malaysia, Thailand and Vietnam to determine the biodiversity and environmental distribution of chloramphenicol-resistant (CmR) mesophilic heterotrophs in Southeast Asian aquaculture...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English English |
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Elsevier
2007
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| Online Access: | http://psasir.upm.edu.my/id/eprint/7282/ http://psasir.upm.edu.my/id/eprint/7282/1/Biodiversity%20of%20chloramphenicol.pdf |
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| author | Huys, Geert Bartie, Kerry Cnockaert, Margo Hoang Oanh, Dang Thi Phuong, Nguyen Thanh Somsiri, Temdoung Chinabut, Supranee Md Yusoff, Fatimah Shariff, Mohamed Giacomini, Mauro Teale, Alan Swings, Jean |
| author_facet | Huys, Geert Bartie, Kerry Cnockaert, Margo Hoang Oanh, Dang Thi Phuong, Nguyen Thanh Somsiri, Temdoung Chinabut, Supranee Md Yusoff, Fatimah Shariff, Mohamed Giacomini, Mauro Teale, Alan Swings, Jean |
| author_sort | Huys, Geert |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In the present study, samples of pond water, sediment and farmed species were collected at 12 fish and shrimp farms in Malaysia, Thailand and Vietnam to determine the biodiversity and environmental distribution of chloramphenicol-resistant (CmR) mesophilic heterotrophs in Southeast Asian aquaculture sites. Following isolation on Iso-Sensitest agar supplemented with 35 μg ml−1 Cm and dereplication using (GTG)5-PCR fingerprinting, 557 genotypically unique CmR strains were subjected to polyphasic identification. The 557 mesophilic heterotrophic CmR isolates represented 18 different genera largely dominated by the genera Escherichia (40.2%), Pseudomonas (11.7%), Acinetobacter (11.1%), Klebsiella (7.5%) and Bacillus (5.9%). A total of 439 CmR isolates were further assigned to 31 described species or species groups, mainly including organisms that have been associated with various human opportunistic infections such as Escherichia coli (n = 219), Pseudomonas putida (n = 47), Klebsiella pneumoniae (n = 38) and Acinetobacter baumannii (n = 23). Strains of Escherichia, and most notably, of E. coli, were the only common group of CmR heterotrophs irrespective of country, sample type or farm type. Together with other predominant but less widespread groups such as acinetobacters and pseudomonads, the results of this biodiversity study suggest that E. coli can be regarded as a potential indicator of Cm resistance in Southeast Asian aquaculture environments.
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| first_indexed | 2025-11-15T07:29:09Z |
| format | Article |
| id | upm-7282 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:29:09Z |
| publishDate | 2007 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-72822015-09-17T03:41:27Z http://psasir.upm.edu.my/id/eprint/7282/ Biodiversity of chloramphenicol-resistant mesophilic heterotrophs from Southeast Asian aquaculture environments Huys, Geert Bartie, Kerry Cnockaert, Margo Hoang Oanh, Dang Thi Phuong, Nguyen Thanh Somsiri, Temdoung Chinabut, Supranee Md Yusoff, Fatimah Shariff, Mohamed Giacomini, Mauro Teale, Alan Swings, Jean In the present study, samples of pond water, sediment and farmed species were collected at 12 fish and shrimp farms in Malaysia, Thailand and Vietnam to determine the biodiversity and environmental distribution of chloramphenicol-resistant (CmR) mesophilic heterotrophs in Southeast Asian aquaculture sites. Following isolation on Iso-Sensitest agar supplemented with 35 μg ml−1 Cm and dereplication using (GTG)5-PCR fingerprinting, 557 genotypically unique CmR strains were subjected to polyphasic identification. The 557 mesophilic heterotrophic CmR isolates represented 18 different genera largely dominated by the genera Escherichia (40.2%), Pseudomonas (11.7%), Acinetobacter (11.1%), Klebsiella (7.5%) and Bacillus (5.9%). A total of 439 CmR isolates were further assigned to 31 described species or species groups, mainly including organisms that have been associated with various human opportunistic infections such as Escherichia coli (n = 219), Pseudomonas putida (n = 47), Klebsiella pneumoniae (n = 38) and Acinetobacter baumannii (n = 23). Strains of Escherichia, and most notably, of E. coli, were the only common group of CmR heterotrophs irrespective of country, sample type or farm type. Together with other predominant but less widespread groups such as acinetobacters and pseudomonads, the results of this biodiversity study suggest that E. coli can be regarded as a potential indicator of Cm resistance in Southeast Asian aquaculture environments. Elsevier 2007 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7282/1/Biodiversity%20of%20chloramphenicol.pdf Huys, Geert and Bartie, Kerry and Cnockaert, Margo and Hoang Oanh, Dang Thi and Phuong, Nguyen Thanh and Somsiri, Temdoung and Chinabut, Supranee and Md Yusoff, Fatimah and Shariff, Mohamed and Giacomini, Mauro and Teale, Alan and Swings, Jean (2007) Biodiversity of chloramphenicol-resistant mesophilic heterotrophs from Southeast Asian aquaculture environments. Research in Microbiology, 158 (3). pp. 228-235. ISSN 0923-2508 http://dx.doi.org/10.1016/j.resmic.2006.12.011 10.1016/j.resmic.2006.12.011 English |
| spellingShingle | Huys, Geert Bartie, Kerry Cnockaert, Margo Hoang Oanh, Dang Thi Phuong, Nguyen Thanh Somsiri, Temdoung Chinabut, Supranee Md Yusoff, Fatimah Shariff, Mohamed Giacomini, Mauro Teale, Alan Swings, Jean Biodiversity of chloramphenicol-resistant mesophilic heterotrophs from Southeast Asian aquaculture environments |
| title | Biodiversity of chloramphenicol-resistant mesophilic heterotrophs from Southeast Asian aquaculture environments |
| title_full | Biodiversity of chloramphenicol-resistant mesophilic heterotrophs from Southeast Asian aquaculture environments |
| title_fullStr | Biodiversity of chloramphenicol-resistant mesophilic heterotrophs from Southeast Asian aquaculture environments |
| title_full_unstemmed | Biodiversity of chloramphenicol-resistant mesophilic heterotrophs from Southeast Asian aquaculture environments |
| title_short | Biodiversity of chloramphenicol-resistant mesophilic heterotrophs from Southeast Asian aquaculture environments |
| title_sort | biodiversity of chloramphenicol-resistant mesophilic heterotrophs from southeast asian aquaculture environments |
| url | http://psasir.upm.edu.my/id/eprint/7282/ http://psasir.upm.edu.my/id/eprint/7282/ http://psasir.upm.edu.my/id/eprint/7282/ http://psasir.upm.edu.my/id/eprint/7282/1/Biodiversity%20of%20chloramphenicol.pdf |