Functional characterization of the gene promoter for an Elaeis guineensis phosphate starvation-inducible, high affinity phosphate transporter in both homologous and heterologous model systems
Oil palm is grown in tropical soils with low bioavailability of Pi. A cDNA clone specifically expressed under phosphate-starvation condition in oil palm roots was identified as a high-affinity phosphate transporter (EgPHT1). The deduced amino acid sequence has 6 transmembrane domains each at the N-...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/72805/ http://psasir.upm.edu.my/id/eprint/72805/1/Functional%20characterization%20of%20the%20gene%20promoter%20for%20an%20Elaeis%20guineensis%20.pdf |
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| author | Ahmadi, Farzaneh Abdullah, Siti Nor Akmar Kadkhodaei, Saeid Shamsuddin, Siti Mariyam Hamzah, Luqman Abdul Aziz, Maheran Abdul Rahman, Zaharah Syed Alwee, Sharifah Shahrul Rabiah |
| author_facet | Ahmadi, Farzaneh Abdullah, Siti Nor Akmar Kadkhodaei, Saeid Shamsuddin, Siti Mariyam Hamzah, Luqman Abdul Aziz, Maheran Abdul Rahman, Zaharah Syed Alwee, Sharifah Shahrul Rabiah |
| author_sort | Ahmadi, Farzaneh |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Oil palm is grown in tropical soils with low bioavailability of Pi. A cDNA clone specifically expressed under phosphate-starvation condition in oil palm roots was identified as a high-affinity phosphate transporter (EgPHT1). The deduced amino acid sequence has 6 transmembrane domains each at the N- and C-termini separated by a hydrophilic linker. Comparison of promoter motifs within 1500 bp upstream of ATG of 10 promoters from high- and low-affinity phosphate transporter from both dicots and monocots including EgPHT1 was performed. The EgPHT1 promoter was fused to β-glucuronidase (GUS) reporter gene and its activity was analysed by histochemical and fluorometric GUS assays in transiently transformed oil palm tissues and T3 homozygous transgenic Arabidopsis plants. In response to Pi-starvation, no GUS activity was detected in oil palm leaves, but a strong inducible activity was observed in the roots (1.4 times higher than the CaMV35S promoter). GUS was specifically expressed in transgenic Arabidopsis roots under Pi deficiency and starvation of the other macronutrients (N and K) did not induce GUS activity. Eight motifs including ABRERATCAL (abscisic-acid responsive), RHERPATEXPA7 (root hair-specific), SURECOREATSULTR11 (sulfur-deficiency response), LTRECOREATCOR15 (temperature-stress response), MYB2CONSENSUSAT and ACGTATERD1 (water-stress response) as well as two novel motifs, 3 (TAAAAAAA) and 26 (TTTTATGT) identified through pattern discovery, occur at significantly higher frequency (p < 0.05) in the high-than the low-affinity phosphate transporter promoters. The Pi deficiency-responsive elements in EgPHT1 includes the P1BS, W-box, E-box and the G-box. Thus, EgPHT1 is important for improving Pi uptake in oil palm with potential for engineering efficient Pi acquisition. |
| first_indexed | 2025-11-15T11:53:52Z |
| format | Article |
| id | upm-72805 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:53:52Z |
| publishDate | 2018 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-728052021-02-09T23:13:52Z http://psasir.upm.edu.my/id/eprint/72805/ Functional characterization of the gene promoter for an Elaeis guineensis phosphate starvation-inducible, high affinity phosphate transporter in both homologous and heterologous model systems Ahmadi, Farzaneh Abdullah, Siti Nor Akmar Kadkhodaei, Saeid Shamsuddin, Siti Mariyam Hamzah, Luqman Abdul Aziz, Maheran Abdul Rahman, Zaharah Syed Alwee, Sharifah Shahrul Rabiah Oil palm is grown in tropical soils with low bioavailability of Pi. A cDNA clone specifically expressed under phosphate-starvation condition in oil palm roots was identified as a high-affinity phosphate transporter (EgPHT1). The deduced amino acid sequence has 6 transmembrane domains each at the N- and C-termini separated by a hydrophilic linker. Comparison of promoter motifs within 1500 bp upstream of ATG of 10 promoters from high- and low-affinity phosphate transporter from both dicots and monocots including EgPHT1 was performed. The EgPHT1 promoter was fused to β-glucuronidase (GUS) reporter gene and its activity was analysed by histochemical and fluorometric GUS assays in transiently transformed oil palm tissues and T3 homozygous transgenic Arabidopsis plants. In response to Pi-starvation, no GUS activity was detected in oil palm leaves, but a strong inducible activity was observed in the roots (1.4 times higher than the CaMV35S promoter). GUS was specifically expressed in transgenic Arabidopsis roots under Pi deficiency and starvation of the other macronutrients (N and K) did not induce GUS activity. Eight motifs including ABRERATCAL (abscisic-acid responsive), RHERPATEXPA7 (root hair-specific), SURECOREATSULTR11 (sulfur-deficiency response), LTRECOREATCOR15 (temperature-stress response), MYB2CONSENSUSAT and ACGTATERD1 (water-stress response) as well as two novel motifs, 3 (TAAAAAAA) and 26 (TTTTATGT) identified through pattern discovery, occur at significantly higher frequency (p < 0.05) in the high-than the low-affinity phosphate transporter promoters. The Pi deficiency-responsive elements in EgPHT1 includes the P1BS, W-box, E-box and the G-box. Thus, EgPHT1 is important for improving Pi uptake in oil palm with potential for engineering efficient Pi acquisition. Elsevier 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72805/1/Functional%20characterization%20of%20the%20gene%20promoter%20for%20an%20Elaeis%20guineensis%20.pdf Ahmadi, Farzaneh and Abdullah, Siti Nor Akmar and Kadkhodaei, Saeid and Shamsuddin, Siti Mariyam and Hamzah, Luqman and Abdul Aziz, Maheran and Abdul Rahman, Zaharah and Syed Alwee, Sharifah Shahrul Rabiah (2018) Functional characterization of the gene promoter for an Elaeis guineensis phosphate starvation-inducible, high affinity phosphate transporter in both homologous and heterologous model systems. Plant Physiology and Biochemistry, 127. 320 - 335. ISSN 0981-9428 https://pubmed.ncbi.nlm.nih.gov/29653435/#affiliation-2 10.1016/j.plaphy.2018.04.004 |
| spellingShingle | Ahmadi, Farzaneh Abdullah, Siti Nor Akmar Kadkhodaei, Saeid Shamsuddin, Siti Mariyam Hamzah, Luqman Abdul Aziz, Maheran Abdul Rahman, Zaharah Syed Alwee, Sharifah Shahrul Rabiah Functional characterization of the gene promoter for an Elaeis guineensis phosphate starvation-inducible, high affinity phosphate transporter in both homologous and heterologous model systems |
| title | Functional characterization of the gene promoter for an Elaeis guineensis phosphate starvation-inducible, high affinity phosphate transporter in both homologous and heterologous model systems |
| title_full | Functional characterization of the gene promoter for an Elaeis guineensis phosphate starvation-inducible, high affinity phosphate transporter in both homologous and heterologous model systems |
| title_fullStr | Functional characterization of the gene promoter for an Elaeis guineensis phosphate starvation-inducible, high affinity phosphate transporter in both homologous and heterologous model systems |
| title_full_unstemmed | Functional characterization of the gene promoter for an Elaeis guineensis phosphate starvation-inducible, high affinity phosphate transporter in both homologous and heterologous model systems |
| title_short | Functional characterization of the gene promoter for an Elaeis guineensis phosphate starvation-inducible, high affinity phosphate transporter in both homologous and heterologous model systems |
| title_sort | functional characterization of the gene promoter for an elaeis guineensis phosphate starvation-inducible, high affinity phosphate transporter in both homologous and heterologous model systems |
| url | http://psasir.upm.edu.my/id/eprint/72805/ http://psasir.upm.edu.my/id/eprint/72805/ http://psasir.upm.edu.my/id/eprint/72805/ http://psasir.upm.edu.my/id/eprint/72805/1/Functional%20characterization%20of%20the%20gene%20promoter%20for%20an%20Elaeis%20guineensis%20.pdf |