Fingerprinting of phospholipid molecular species from human milk and infant formula using HILIC-ESI-IT-TOF-MS and discriminatory analysis by principal component analysis
Phospholipid composition in the milk fat globule membrane (MFGM) fluctuates during the entire lactation period in order to suit the growing needs of newborn infants. The present study elucidated and relatively quantified phospholipid molecular species extracted from human milk (HM), mature human mil...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
American Chemical Society
2018
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/72757/ http://psasir.upm.edu.my/id/eprint/72757/1/Fingerprinting%20of%20phospholipid%20molecular%20species%20from%20human%20milk%20and%20infant%20formula%20using%20HILIC-ESI-IT-TOF-MS%20and%20discriminatory%20analysis%20by%20principal%20component%20analysis.pdf |
| _version_ | 1848857195371823104 |
|---|---|
| author | Jiang, Chenyu Ma, Baokai Song, Shuang Lai, Oi Ming Cheong, Ling Zhi |
| author_facet | Jiang, Chenyu Ma, Baokai Song, Shuang Lai, Oi Ming Cheong, Ling Zhi |
| author_sort | Jiang, Chenyu |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Phospholipid composition in the milk fat globule membrane (MFGM) fluctuates during the entire lactation period in order to suit the growing needs of newborn infants. The present study elucidated and relatively quantified phospholipid molecular species extracted from human milk (HM), mature human milk (MHM), and infant formulas (with or without MFGM supplementation) using hydrophilic liquid chromatography-electrospray ionization-ion trap-time of flight-mass spectrometry (HILIC-ESI-IT-TOF-MS) system. Principal component analysis was used to clarify the differences between phospholipid composition in HM, MHM, and infant formulas. HM and MHM contained high concentrations of sphingomyeline (HM: 107.61 μg/mL, MHM: 227.18 μg/mL), phosphatidylcholine (HM: 59.96 μg/mL, MHM: 50.77 μg/mL), and phosphatidylethanolamine (PE) (HM: 25.24 μg/mL, MHM: 31.76 μg/mL). Significant concentrations (<300 ng/mL) of arachidonic, eicosapentanoic, and docosahexanoic acids were found to esterify to PE in HM and MHM. Meanwhile, all infant formulas were found to contain high concentrations of phosphatidic acids indicating the possibility of degradation of the fortified MFGM either during processing or storage of the infant formulas. |
| first_indexed | 2025-11-15T11:53:41Z |
| format | Article |
| id | upm-72757 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:53:41Z |
| publishDate | 2018 |
| publisher | American Chemical Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-727572021-02-01T11:07:23Z http://psasir.upm.edu.my/id/eprint/72757/ Fingerprinting of phospholipid molecular species from human milk and infant formula using HILIC-ESI-IT-TOF-MS and discriminatory analysis by principal component analysis Jiang, Chenyu Ma, Baokai Song, Shuang Lai, Oi Ming Cheong, Ling Zhi Phospholipid composition in the milk fat globule membrane (MFGM) fluctuates during the entire lactation period in order to suit the growing needs of newborn infants. The present study elucidated and relatively quantified phospholipid molecular species extracted from human milk (HM), mature human milk (MHM), and infant formulas (with or without MFGM supplementation) using hydrophilic liquid chromatography-electrospray ionization-ion trap-time of flight-mass spectrometry (HILIC-ESI-IT-TOF-MS) system. Principal component analysis was used to clarify the differences between phospholipid composition in HM, MHM, and infant formulas. HM and MHM contained high concentrations of sphingomyeline (HM: 107.61 μg/mL, MHM: 227.18 μg/mL), phosphatidylcholine (HM: 59.96 μg/mL, MHM: 50.77 μg/mL), and phosphatidylethanolamine (PE) (HM: 25.24 μg/mL, MHM: 31.76 μg/mL). Significant concentrations (<300 ng/mL) of arachidonic, eicosapentanoic, and docosahexanoic acids were found to esterify to PE in HM and MHM. Meanwhile, all infant formulas were found to contain high concentrations of phosphatidic acids indicating the possibility of degradation of the fortified MFGM either during processing or storage of the infant formulas. American Chemical Society 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72757/1/Fingerprinting%20of%20phospholipid%20molecular%20species%20from%20human%20milk%20and%20infant%20formula%20using%20HILIC-ESI-IT-TOF-MS%20and%20discriminatory%20analysis%20by%20principal%20component%20analysis.pdf Jiang, Chenyu and Ma, Baokai and Song, Shuang and Lai, Oi Ming and Cheong, Ling Zhi (2018) Fingerprinting of phospholipid molecular species from human milk and infant formula using HILIC-ESI-IT-TOF-MS and discriminatory analysis by principal component analysis. Journal of Agricultural and Food Chemistry, 66 (27). 7131 - 7138. ISSN 0021-8561; ESSN: 1520-5118 https://pubs.acs.org/doi/10.1021/acs.jafc.8b01393# 10.1021/acs.jafc.8b01393 |
| spellingShingle | Jiang, Chenyu Ma, Baokai Song, Shuang Lai, Oi Ming Cheong, Ling Zhi Fingerprinting of phospholipid molecular species from human milk and infant formula using HILIC-ESI-IT-TOF-MS and discriminatory analysis by principal component analysis |
| title | Fingerprinting of phospholipid molecular species from human milk and infant formula using HILIC-ESI-IT-TOF-MS and discriminatory analysis by principal component analysis |
| title_full | Fingerprinting of phospholipid molecular species from human milk and infant formula using HILIC-ESI-IT-TOF-MS and discriminatory analysis by principal component analysis |
| title_fullStr | Fingerprinting of phospholipid molecular species from human milk and infant formula using HILIC-ESI-IT-TOF-MS and discriminatory analysis by principal component analysis |
| title_full_unstemmed | Fingerprinting of phospholipid molecular species from human milk and infant formula using HILIC-ESI-IT-TOF-MS and discriminatory analysis by principal component analysis |
| title_short | Fingerprinting of phospholipid molecular species from human milk and infant formula using HILIC-ESI-IT-TOF-MS and discriminatory analysis by principal component analysis |
| title_sort | fingerprinting of phospholipid molecular species from human milk and infant formula using hilic-esi-it-tof-ms and discriminatory analysis by principal component analysis |
| url | http://psasir.upm.edu.my/id/eprint/72757/ http://psasir.upm.edu.my/id/eprint/72757/ http://psasir.upm.edu.my/id/eprint/72757/ http://psasir.upm.edu.my/id/eprint/72757/1/Fingerprinting%20of%20phospholipid%20molecular%20species%20from%20human%20milk%20and%20infant%20formula%20using%20HILIC-ESI-IT-TOF-MS%20and%20discriminatory%20analysis%20by%20principal%20component%20analysis.pdf |