Fabrication, characterization, and functionalization of single-walled carbon nanotube conjugated with tamoxifen and its anticancer potential against human breast cancer cells
In this experiment, we aimed to fabricate SWCNT conjugated with tamoxifen and evaluated its anticancer potential against human breast cancer cells (MCF-7). The results showed that SWCNT was synthetized successfully using chemical vapor deposition (CVD) method. The results of Raman spectroscopy, SEM,...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/72704/ http://psasir.upm.edu.my/id/eprint/72704/1/Fabrication%2C%20characterization%2C%20and%20functionalization%20of%20singl.pdf |
| _version_ | 1848857183331024896 |
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| author | Oskoueian, Arshin Matori, Khamirul Amin Bayat, Saadi Oskoueian, Ehsan Ostovan, Farhad Toozandehjani, Meysam |
| author_facet | Oskoueian, Arshin Matori, Khamirul Amin Bayat, Saadi Oskoueian, Ehsan Ostovan, Farhad Toozandehjani, Meysam |
| author_sort | Oskoueian, Arshin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In this experiment, we aimed to fabricate SWCNT conjugated with tamoxifen and evaluated its anticancer potential against human breast cancer cells (MCF-7). The results showed that SWCNT was synthetized successfully using chemical vapor deposition (CVD) method. The results of Raman spectroscopy, SEM, and TEM analyses confirmed the synthesis of highly pure SWCNT. The functionalization of SWCNT was performed by oxidizing of SWCNT, attachment of polyethylene glycol (PEG) to oxidized SWCNT, and attachment of azelaic acid to the polyethylene glycol group. As a result, the SWCNT with free functional carboxylic acid and hydroxyl groups (SWCNT-PEG) was developed. The SWCNT-PEG was then conjugated with tamoxifen (SWCNT-PEG-TAM). The FT-IR together with NMR results confirmed the conjugation of tamoxifen to functionalized SWCNT (SWCNT-PEG-TAM). The cytotoxic concentrations (CC50) of SWCNT-PEG, tamoxifen, and SWCNT-PEG-TAM were >100, , and μg/ml, respectively. Linking tamoxifen to functionalized SWCNT enhanced the cytotoxic action of tamoxifen against breast cancer cells up to 2.3 times. The results of the morphological examination and caspase-3 activity confirmed the higher cytotoxic action of SWCNT-PEG-TAM as compared to free tamoxifen. The results obtained in this study indicated that this delivery system enhanced the therapeutic effects and anticancer potential of tamoxifen against human breast cancer cells. |
| first_indexed | 2025-11-15T11:53:30Z |
| format | Article |
| id | upm-72704 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:53:30Z |
| publishDate | 2018 |
| publisher | Hindawi |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-727042021-01-11T09:34:49Z http://psasir.upm.edu.my/id/eprint/72704/ Fabrication, characterization, and functionalization of single-walled carbon nanotube conjugated with tamoxifen and its anticancer potential against human breast cancer cells Oskoueian, Arshin Matori, Khamirul Amin Bayat, Saadi Oskoueian, Ehsan Ostovan, Farhad Toozandehjani, Meysam In this experiment, we aimed to fabricate SWCNT conjugated with tamoxifen and evaluated its anticancer potential against human breast cancer cells (MCF-7). The results showed that SWCNT was synthetized successfully using chemical vapor deposition (CVD) method. The results of Raman spectroscopy, SEM, and TEM analyses confirmed the synthesis of highly pure SWCNT. The functionalization of SWCNT was performed by oxidizing of SWCNT, attachment of polyethylene glycol (PEG) to oxidized SWCNT, and attachment of azelaic acid to the polyethylene glycol group. As a result, the SWCNT with free functional carboxylic acid and hydroxyl groups (SWCNT-PEG) was developed. The SWCNT-PEG was then conjugated with tamoxifen (SWCNT-PEG-TAM). The FT-IR together with NMR results confirmed the conjugation of tamoxifen to functionalized SWCNT (SWCNT-PEG-TAM). The cytotoxic concentrations (CC50) of SWCNT-PEG, tamoxifen, and SWCNT-PEG-TAM were >100, , and μg/ml, respectively. Linking tamoxifen to functionalized SWCNT enhanced the cytotoxic action of tamoxifen against breast cancer cells up to 2.3 times. The results of the morphological examination and caspase-3 activity confirmed the higher cytotoxic action of SWCNT-PEG-TAM as compared to free tamoxifen. The results obtained in this study indicated that this delivery system enhanced the therapeutic effects and anticancer potential of tamoxifen against human breast cancer cells. Hindawi 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72704/1/Fabrication%2C%20characterization%2C%20and%20functionalization%20of%20singl.pdf Oskoueian, Arshin and Matori, Khamirul Amin and Bayat, Saadi and Oskoueian, Ehsan and Ostovan, Farhad and Toozandehjani, Meysam (2018) Fabrication, characterization, and functionalization of single-walled carbon nanotube conjugated with tamoxifen and its anticancer potential against human breast cancer cells. Journal of Nanomaterials, 2018. art. no. 8417016. pp. 1-14. ISSN 1687-4110; ESSN: 1687-4129 https://www.hindawi.com/journals/jnm/2018/8417016/ 10.1155/2018/8417016 |
| spellingShingle | Oskoueian, Arshin Matori, Khamirul Amin Bayat, Saadi Oskoueian, Ehsan Ostovan, Farhad Toozandehjani, Meysam Fabrication, characterization, and functionalization of single-walled carbon nanotube conjugated with tamoxifen and its anticancer potential against human breast cancer cells |
| title | Fabrication, characterization, and functionalization of single-walled carbon nanotube conjugated with tamoxifen and its anticancer potential against human breast cancer cells |
| title_full | Fabrication, characterization, and functionalization of single-walled carbon nanotube conjugated with tamoxifen and its anticancer potential against human breast cancer cells |
| title_fullStr | Fabrication, characterization, and functionalization of single-walled carbon nanotube conjugated with tamoxifen and its anticancer potential against human breast cancer cells |
| title_full_unstemmed | Fabrication, characterization, and functionalization of single-walled carbon nanotube conjugated with tamoxifen and its anticancer potential against human breast cancer cells |
| title_short | Fabrication, characterization, and functionalization of single-walled carbon nanotube conjugated with tamoxifen and its anticancer potential against human breast cancer cells |
| title_sort | fabrication, characterization, and functionalization of single-walled carbon nanotube conjugated with tamoxifen and its anticancer potential against human breast cancer cells |
| url | http://psasir.upm.edu.my/id/eprint/72704/ http://psasir.upm.edu.my/id/eprint/72704/ http://psasir.upm.edu.my/id/eprint/72704/ http://psasir.upm.edu.my/id/eprint/72704/1/Fabrication%2C%20characterization%2C%20and%20functionalization%20of%20singl.pdf |